LEGITIMACY OF PROGENIES USING SSR MARKERS AS CONTROLLING SYSTEM AND EARLIER SELECTION IN THE OIL PALM NURSERY (Elaeis guineensis JACQ.)

2018 ◽  
Vol 25 (1) ◽  
pp. 21-30
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Abdul Razak Purba
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Oil Palm ◽  
Dna Markers ◽  
Elaeis Guineensis ◽  
Gene Marker ◽  
Controlling System ◽  
Crossing Probability ◽  
Simple Sequence

Information of legitimacy of oil palm progenies is important to guaranty the quality and to control commercial seeds procedures. A true and legitimate cross will produce progeny which has a combination of their parent's allele. The information could be obtained early in the nursery stage through DNA fingerprinting analysis. Simple Sequence Repeats (SSR) is one of DNA markers used for DNA fingerprinting, since the marker system has advantages to acquire information of allele per individual in population and efficiency diverse allele of progeny and their parents. The aim of the research is to obtain legitimacy of 12 progenies analyzing in the oil palm nursery stage. Thirteen SSR markers were used to analyze 12 crossings number of oil palm. The genotypes data by alleles of SSR inferred and quantified using Gene Marker® Software version 2.4.0 Soft Genetics® LLC and analyzed based on Mendel's Law of Segregation. The result showed based on heredity pattern of progeny and their parent's allele that progenies H were indicated genetically derived from their known parents while progenies from A and G indicated as illegitimate crossing. Probability value for legitimacy of progenies of 9 other crosses has 0.031 and 0.5. Legitimacy analysis of progeny using SSR markers could be used to control the quality of crossing material and earlier selection in the oil palm nursery.

scholarly journals LEGITIMACY OF PROGENIES USING SSR MARKERS AS CONTROLLING SYSTEM AND EARLIER SELECTION IN THE OIL PALM NURSERY (Elaeis guineensis JACQ.)

2017 ◽  
Vol 25 (1) ◽  
pp. 21-30
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Abdul Razak Purba
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Oil Palm ◽  
Dna Markers ◽  
Elaeis Guineensis ◽  
Gene Marker ◽  
Controlling System ◽  
Crossing Probability ◽  
Simple Sequence

Information of legitimacy of oil palm progenies is important to guaranty the quality and to control commercial seeds procedures. A true and legitimate cross will produce progeny which has a combination of their parent's allele. The information could be obtained early in the nursery stage through DNA fingerprinting analysis. Simple Sequence Repeats (SSR) is one of DNA markers used for DNA fingerprinting, since the marker system has advantages to acquire information of allele per individual in population and efficiency diverse allele of progeny and their parents. The aim of the research is to obtain legitimacy of 12 progenies analyzing in the oil palm nursery stage. Thirteen SSR markers were used to analyze 12 crossings number of oil palm. The genotypes data by alleles of SSR inferred and quantified using Gene Marker® Software version 2.4.0 Soft Genetics® LLC and analyzed based on Mendel's Law of Segregation. The result showed based on heredity pattern of progeny and their parent's allele that progenies H were indicated genetically derived from their known parents while progenies from A and G indicated as illegitimate crossing. Probability value for legitimacy of progenies of 9 other crosses has 0.031 and 0.5. Legitimacy analysis of progeny using SSR markers could be used to control the quality of crossing material and earlier selection in the oil palm nursery.

scholarly journals GENETIC TRACEABILITY OF OIL PALM PROGENIES (Elaeis guineensis JACQ.) USING SIMPLE SEQUENCE REPEAT (SSR)

2016 ◽  
Vol 24 (3) ◽  
pp. 103-114
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Yurna Yenni ◽  
Sujadi Sujadi
Keyword(s):  
Ssr Markers ◽  
Oil Palm ◽  
Simple Sequence Repeat ◽  
Elaeis Guineensis ◽  
Gene Marker ◽  
Sequence Repeat ◽  
Fragment Analysis ◽  
Traceability Analysis ◽  
Simple Sequence ◽  
Important Activity

Progenies traceability status becomes an important activity in the oil palm breeding program, especially to obtain propriety of individual and progenies population with their parents. This study is concerned to analyze genetic traceability based on pattern and allele size of their parent using Simple Sequence Repeat (SSR) markers. There were 90 palms from 7 progenies populations in the AD02S, AD03S Adolina and MA22S Marihat Estate PT. Perkebunan Nusantara IV used in this research. Leaf and pollen samples were used to get genomic DNA. Then, DNA genomic was amplified using multiplexing method of 8 SSR markers and fluorescence labels of 6-FAM, HEX, and NED. Fragment analysis and extracted genotype data was obtained using Gene Marker® versi 2.4.0 Soft Genetics® LLC program. Genetic traceability analysis was based on alelle segregation pattern of Mendelian Law. The results described inappropriate alleles 4 individuals from 3 progenies (N, P, and S). Those individuals were palmrow of 22-30 in the AD02S; 29-27 in the MA22S; 9-22 and 9-28 in the AD02S. Other 4 progenies showed an appropriate segregation of genotype with their parents, which are crosses number of M, Q, R, and O.

GENETIC TRACEABILITY OF OIL PALM PROGENIES (Elaeis guineensis JACQ.) USING SIMPLE SEQUENCE REPEAT (SSR)

2018 ◽  
Vol 24 (3) ◽  
pp. 103-114
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Yurna Yenni ◽  
Sujadi Sujadi
Keyword(s):  
Ssr Markers ◽  
Oil Palm ◽  
Simple Sequence Repeat ◽  
Elaeis Guineensis ◽  
Gene Marker ◽  
Sequence Repeat ◽  
Fragment Analysis ◽  
Traceability Analysis ◽  
Simple Sequence ◽  
Important Activity

Progenies traceability status becomes an important activity in the oil palm breeding program, especially to obtain propriety of individual and progenies population with their parents. This study is concerned to analyze genetic traceability based on pattern and allele size of their parent using Simple Sequence Repeat (SSR) markers. There were 90 palms from 7 progenies populations in the AD02S, AD03S Adolina and MA22S Marihat Estate PT. Perkebunan Nusantara IV used in this research. Leaf and pollen samples were used to get genomic DNA. Then, DNA genomic was amplified using multiplexing method of 8 SSR markers and fluorescence labels of 6-FAM, HEX, and NED. Fragment analysis and extracted genotype data was obtained using Gene Marker® versi 2.4.0 Soft Genetics® LLC program. Genetic traceability analysis was based on alelle segregation pattern of Mendelian Law. The results described inappropriate alleles 4 individuals from 3 progenies (N, P, and S). Those individuals were palmrow of 22-30 in the AD02S; 29-27 in the MA22S; 9-22 and 9-28 in the AD02S. Other 4 progenies showed an appropriate segregation of genotype with their parents, which are crosses number of M, Q, R, and O.

scholarly journals IDENTIFIKASI KERAGAMAN MOLEKULER MATERIALGENETIK KELAPA SAWIT (Elaeis guineensis Jacq.) BERDASARKAN MARKA SSR (Simple Sequence Repeats)

2017 ◽  
Vol 4 (1) ◽  
pp. 57-64
Author(s):  
Indra Syahputra
Keyword(s):  
Plant Breeding ◽  
Ssr Markers ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Genetic Material ◽  
Breeding Programs ◽  
Molecular Variants ◽  
One Step ◽  
The One ◽  
Simple Sequence

Oil Palm very important for Indonesia. The plant breeding programs were done to found thebest varieties. The identification of molecular variants is the one step of plant breeding programs.The purpose of this research was to analysis molecular variants of the genetic material of oil palm-based on 4 SSR markers, there were mCnCIR0038, mEgCIR0894, mEgCIR3292 and mEgCIR3663. DARwin 6.0 was used to analysis of the data. The results of this research showedthat genetic molecular variants based on SSR markers were 41.68%.

scholarly journals DNA Fingerprinting and Genotyping of Cotton Varieties Using SSR Markers

2012 ◽  
Vol 40 (2) ◽  
pp. 261 ◽  
Author(s):  
Mohammad Nazrul ISLAM ◽  
Md. Rezwan MOLLA ◽  
Md. Motiar ROHMAN ◽  
Mirza HASANUZZAMAN ◽  
Shah Mohammad Naimul ISLAM ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Selective Breeding ◽  
Plant Genetic Resources ◽  
Microsatellite Dna Markers ◽  
Simple Sequence

DNA fingerprinting and genetic diversity analysis helps direct selective breeding and conservation of plant species. Since simple sequence repeats (SSR) markers are co-dominant, they can predict level of genetic diversity and thereby protect plant genetic resources of a region. Keeping the aforesaid rationale in mind, we worked on molecular characterization of eight cotton varieties in Bangladesh using simple sequence repeat (SSR) or microsatellite DNA markers. All the three microsatellite DNA markers were found to be polymorphic, extracting a total of eight alleles with an average of 2.67 alleles per locus in the present study. Allele sizes were as 149-155 bp, 178-198 bp and 140-202 bp for the loci BNL1551, BNL1721 and BNL2960, respectively. Polymorphism Information Content (PIC) values were ranged from 0.469 to 0.531. UPGMA dendrogram separated 8 varieties of cotton into two clusters. One cluster contained six varieties CB-1, CB-2, CB-3, CB-7, CB-9 and CB-10 while other two varieties CB-5 and HC 1 formed another cluster. The findings of this study would provide a useful guide for selecting specific germplasm with distinct genetic background for diversifying cotton breeding program in Bangladesh.

Identification of QTLs in oil palm (Elaeis guineensis Jacq.) using SSR markers through association mapping

2020 ◽  
Vol 99 (1) ◽  
Author(s):  
H. P. Bhagya ◽  
B. Kalyana Babu ◽  
P. M. Gangadharappa ◽  
Mahantesha B. N. Naika ◽  
D. Satish ◽  
...  
Keyword(s):  
Association Mapping ◽  
Ssr Markers ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

scholarly journals PENGARUH KONSENTRASI GIBERELIN (GA3) DENGAN WAKTU APLIKASI SEBELUM PANEN TERHADAP MUTU FISIK TANDAN BUAH SEGAR KELAPA SAWIT (Elaeis guineensis Jacq.)

2019 ◽  
Vol 1 (2) ◽  
pp. 62-70
Author(s):  
Sri Murti Tarigan ◽  
Eka Bobby Febrianto ◽  
Lufy Andria Cik
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Design Method ◽  
Block Design ◽  
Fruit Weight ◽  
Fresh Fruit ◽  
Physical Damage ◽  
Physical Quality ◽  
Fresh Fruit Bunches

Physical damage to fruit on oil palm fresh fruit bunches due to harvest and postharvest activities include injured fruit mesocarp, fruit weight loss, and loss in fruit bunches. One of the postharvest handlings that can maintain the physical quality of the fruit is by giving gibberellins (GA3). This research aims to determine the effect of the concentration of gibberellins (GA3) whit application time before harvest on the physical quality of postharvest oil palm fresh fruit bunches. The research was conducted at Afdeling VI Garden Cot Girek PT. Perkebunan Nusantara I and in the Cot Girek Palm Oil Factory laboratory. The time of the research was conducted in June until July 2019. The study used a non factorial randomized block design method with 4 treatment levels (0 mg/l, 50 mg/l, 100 mg/l, and 150 mg/l) with 3 replications. The administration of gibberellins has a significant effect on fruit loss and fruit weight reduction with the best concentration of gibberellins is 50 ppm but does not have a significant effect on pH, moisture content, and fruit free fatty acids.

scholarly journals Map Construction Of Simple Sequence Repeat Developed For Introgression Philippine Downy Mildew Resistance Gene In Maize

Zuriat ◽  
2015 ◽  
Vol 12 (2) ◽  
Author(s):  
D. Ruswandi ◽  
D. M. Hautea ◽  
A. M. Salazar ◽  
R. M. Lantican ◽  
A. L. Carpena ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Ssr Markers ◽  
Dna Markers ◽  
Simple Sequence Repeat ◽  
Average Distance ◽  
Downy Mildew Resistance ◽  
Plant Genomes ◽  
Map Construction ◽  
Molecular Map ◽  
Simple Sequence

Simple sequence repeats (SSRs) is an important DNA markers for mapping plant genomes particularly in mapping quantitative trait loci (QTL). To identify and map SSR markers for introgression resistance gene against Peronosclerospora philippinensis in maize, the parental materials (P 345 and Pi 23) together with 142 genotypes of BC1F1 of Pi 23 ×P 345 population were used. A total of 50 SSR markers were genotyped. However, only thirty-three markers were integrated into a molecular map together with RFLP and RGA markers. A molecular map of SSR was successfully constructed together with RFLP and RGA into 10 chromosomes. It covered a total map length of 1822 cM with an average distance of 18.22 cM between markers.

scholarly journals Genetic variation and DNA fingerprinting of durian types in Malaysia using simple sequence repeat (SSR) markers

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4266 ◽  
Author(s):  
Ging Yang Siew ◽  
Wei Lun Ng ◽  
Sheau Wei Tan ◽  
Noorjahan Banu Alitheen ◽  
Soon Guan Tan ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Resources ◽  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Dna Sequences ◽  
Simple Sequence Repeat ◽  
Germplasm Collection ◽  
Direct Result ◽  
Sequence Repeat ◽  
Simple Sequence

Durian (Durio zibethinus) is one of the most popular tropical fruits in Asia. To date, 126 durian types have been registered with the Department of Agriculture in Malaysia based on phenotypic characteristics. Classification based on morphology is convenient, easy, and fast but it suffers from phenotypic plasticity as a direct result of environmental factors and age. To overcome the limitation of morphological classification, there is a need to carry out genetic characterization of the various durian types. Such data is important for the evaluation and management of durian genetic resources in producing countries. In this study, simple sequence repeat (SSR) markers were used to study the genetic variation in 27 durian types from the germplasm collection of Universiti Putra Malaysia. Based on DNA sequences deposited in Genbank, seven pairs of primers were successfully designed to amplify SSR regions in the durian DNA samples. High levels of variation among the 27 durian types were observed (expected heterozygosity, HE = 0.35). The DNA fingerprinting power of SSR markers revealed by the combined probability of identity (PI) of all loci was 2.3×10−3. Unique DNA fingerprints were generated for 21 out of 27 durian types using five polymorphic SSR markers (the other two SSR markers were monomorphic). We further tested the utility of these markers by evaluating the clonal status of shared durian types from different germplasm collection sites, and found that some were not clones. The findings in this preliminary study not only shows the feasibility of using SSR markers for DNA fingerprinting of durian types, but also challenges the current classification of durian types, e.g., on whether the different types should be called “clones”, “varieties”, or “cultivars”. Such matters have a direct impact on the regulation and management of durian genetic resources in the region.

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