Electroporation-Mediated Gene Transfer to Protoplasts and Regeneration of Transgenic Plants

The introduction of foreign DNA into the plant genome by Agrobacterium tumefaciens is a promising technique of targeted gene transfer which depends on good working regeneration system. The aim of the work was to elaborate the system for efficient adventitious organogenesis and transgenic plant regeneration in Rubus fruticosus L. using explants from mature plants. Regeneration of putative transgenic shoots took place from flag explants cultivated vertically on MS medium with 1 mg l-1 TDZ and 0.02 mg l-1 IBA followed by transfer on MS medium with 1 mg l-1 BAP, 0.02 mg l-1 IBA and 0.1 mg l-1 GA3 supplemented with 10-15 mg l-1 hygromycin after transformation by A. tumefaciens strain LBA 4404 carrying plasmid pCambia 1304. Four putative transgenic plants of cv. 'Cacanska Bestrna' were rooted and acclimatized.

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Impacts of transgenic plants and microorganisms on soil biota

New Zealand Plant Protection ◽

10.30843/nzpp.2001.54.3725 ◽

2001 ◽

Vol 54 ◽

pp. 105-110 ◽

Cited By ~ 5

Author(s):

M. O'Callaghan ◽

T.R. Glare

Keyword(s):

Gene Transfer ◽

Horizontal Gene Transfer ◽

Transgenic Plants ◽

Recent Work ◽

Soil Bacteria ◽

Genetically Modified ◽

Soil Invertebrates ◽

Soil Biota ◽

Soil Ecosystem ◽

Natural Systems

Little is known about the impacts of transgenic plants and microorganisms on soil biota and processes despite the large number of field releases of transgenic crops Recent work has shown that transgenic plants can cause changes in the soil biota (both invertebrates and microorganisms) associated with these plants Often the changes are transient but their impact on the soil ecosystem remains unknown Horizontal gene transfer from genetically modified (GM) bacteria to indigenous soil bacteria has been demonstrated but movement of genes from transgenic plants if it occurs at all has not yet been detected in natural systems Soil invertebrates appear to play an important role in horizontal gene transfer between bacteria in soil

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Fertile transgenic plants from direct gene transfer to protoplasts of Lolium perenne L. and Lolium multiflorum Lam.

Journal of Plant Physiology ◽

10.1016/s0176-1617(97)80041-7 ◽

1997 ◽

Vol 151 (1) ◽

pp. 83-90 ◽

Cited By ~ 27

Author(s):

G.R. Wang ◽

H. Binding ◽

U.K. Posselt

Keyword(s):

Gene Transfer ◽

Transgenic Plants ◽

Lolium Perenne ◽

Lolium Multiflorum ◽

Direct Gene Transfer ◽

Lolium Perenne L ◽

Direct Gene

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Comparative Review of Plant Transformation Techniques

Journal of Advances in Biology & Biotechnology ◽

10.9734/jabb/2021/v24i1030243 ◽

2021 ◽

pp. 1-18

Author(s):

A. O. Okpe ◽

F. A. Nkaa

Keyword(s):

Gene Transfer ◽

Transgenic Plants ◽

Plant Species ◽

Plant Transformation ◽

Comparative Review ◽

Practical Tool ◽

Foreign Genes ◽

Diverse Plant Species ◽

Transformation Systems ◽

Direct And Indirect Methods

Plant transformation is now an important biotechnological tool in plant biology and a practical tool for transgenic plant development. There are many verified methods for stable introduction of novel genes into the nuclear genomes of diverse plant species. As a result, gene transfer and regeneration of transgenic plants are no longer the factors limiting the development and application of practical transformation systems for many plant species. However, the desire for higher transformation efficiency has stimulated work on not only improving various existing methods but also in inventing novel methods. Different methods of transferring the gene into plant cells have been developed and continuous efforts have been made to increase its efficiency. Both direct and indirect methods of gene transfer have their own merits and demerits. Efforts have been made continuously to eliminate drawbacks and to develop an easy and eco-friendly method to transfer foreign genes. Many methods of genetic transformation have been proposed and tried in the laboratories, but most of them result to transient expressions. However, transformation work based on particle bombardment with DNA coated micro projectiles and Agrobacterium mediated transformation have proved to be promising in producing stable transgenic plants from a range of plant species.

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