Matrix Effects in the Quantitative Elemental Analysis of Plastic-Embedded and Ashed Biological Tissue by SIMS

1986 ◽  
pp. 124-126 ◽  
Author(s):  
J. T. Brenna ◽  
G. H. Morrison
Keyword(s):  
Elemental Analysis ◽  
Biological Tissue ◽  
Matrix Effects ◽  
Quantitative Elemental Analysis

A Non-Dispersive X-ray Fluorescence Unit for the Analysis of Biological Tissue Sections

1957 ◽  
Vol 1 ◽  
pp. 297-313
Author(s):  
Theodore Hall
Keyword(s):  
Proportional Counter ◽  
Biological Tissue ◽  
Matrix Effects ◽  
Pulse Height ◽  
Fluorescence Analysis ◽  
Mineral Elements ◽  
X Ray ◽  
Tissue Sections ◽  
Characteristic Lines ◽  
Secondary Radiator

AbstractAn X-ray fluorescence analysis unit has been designed and built especially for the measurement of certain mineral elements in individual biological tissue sections. Such a section may contain in the neighborhood of 10-10 grams of an element of interest, in a concentration in the range of 1-100 p.p.m.The unit consists of a special high-power X-ray tube with a builtin interchangeable secondary radiator, which irradiates the speciman with the characteristic lines of the radiator element) and a proportional counter and pulse-height analyzer system, which provides analysis of the X-ray spectrum emitted by the specimen. Because the emitted spectrum is greatly simplified by the use of an appropriate radiator element, a diffracting crystal can be omitted, permitting a great increase in absolute sensitivity.The system is feasible only because of two peculiarities of our biological specimens: they are so thin that matrix effects are negligible, and they consist essentially of a few mineral elements in a light matrix.Design considerations, calibration procedures, procedures for the analysis of the observed proportional counter pulse-height spectra and results to date will be discussed.

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