Food nanoparticles from rice vinegar: isolation, characterization, and antioxidant activities

Abundant nanostructures have been constantly found in various foods, like vinegar, tea, coffee, and milk. However, these structures largely remain unexplored and even been eliminated for stability reasons in food industry. Here we report the isolation, characterization, and antioxidant activities of food nanoparticles (NPs) carrying polyphenols from Chinese rice vinegar. Using a gel-chromatography-based isolation protocol, the vinegar was separated into three major fractions. They were identified as spherical NPs (P1), lollipop-like NPs (P2) and spherical microparticles (P3) with average hydrodynamic diameter of 210, 245,1643 nm, separately. The former two fractions accounted for the major parts of dry matter in the vinegar. The P1-NPs fraction was composed of proteins, carbohydrates, and a high number of polyphenols (15 wt%), demonstrated potent antioxidant activity as determined by ABTS and ORAC assays. Moreover, they effectively quenched peroxyl free radicals in peritoneal macrophages and promoted cellular growth. The P2 fraction contained majority of organic acids, esters and mineral elements of the vinegar. It demonstrated the NPs are bioactive units of the rice vinegar, inspiring the development of novel functional nanomaterials with nutraceutical and pharmaceutical applications.

Correlations of gestational hemoglobin, placental mineral elements and reproductive performances in pregnant sows

Iron status of sows has a great influence on reproductive performance. Iron deficiency reduces reproductive performance and newborn piglet survival rate of sow. The hemoglobin is a potential predictor for iron status of sows and is convenient for rapid detection in pig farms. However, the relationship between iron status, hemoglobin, placental trace elements and reproductive performance remains unclear. In this study, the hemoglobin and reproductive performance of more than 500 sows with 1 st to 6 th parities at different gestation stages (25, 55, 75, 95, 110 days of gestation) in two large-scale sow farms were collected, and content of placental Fe, Zn, Mn and Cu was analyzed. The results show hemoglobin of sows during pregnancy (d 75, d 95, d 110) decreased significantly (P < 0.001). As the parity increases, the hemoglobin levels of sows at d 25, d 55 of gestation and placental mineral element contents included Fe, Zn, Mn and Cu at delivery decreased (P < 0.05), while the litter size, birth alive and litter weights increased gradually (P < 0.001). Furthermore, hemoglobin during pregnancy had a negative linear correlation with litter weight and average weight (P < 0.05), higher hemoglobin at d 25 of gestation may reduce the number of stillbirths (P = 0.05), but higher hemoglobin at d 110 of gestation was tend to be benefit for the birth (P = 0.01). And there was a significant positive linear correlation between hemoglobin at d 110 of gestation and placental Fe and Mn levels (P = 0.002, P = 0.013). There was also a significant positive linear correlation among Fe, Zn, Mn and Cu in the placenta (P < 0.001). The levels of Fe, Zn, and Mn in placental at delivery were positive related to the average weight of the fetus (P = 0.048, P = 0.027, P = 0.047), and placental Cu was linearly correlated with litter size (P = 0.029). Our research revealed the requirements for iron during gestation were varied in different gestation periods and parities. The feeds should be adjusted according to the gestation periods, parities or iron status to meet the iron requirements of sows and fetal pigs.

Mineral composition of wheat species as influenced by different fertilizer sources and different weed control practices

This research was carried out to determine the effects of agronomic practices on the mineral composition of organically-grown wheat species. In terms of all nutrients evaluated, the mineral content of wheat showed significant differences according to crop years, varieties, weed control methods and fertilizer sources. As the average of all factors, the Cu, Fe, Mn, Se, Zn, Cd, Co, Cr, Ni and Pb contents of the ground wheat grain were; 3.93, 42.8, 79.6, 0.549, 11.34, 0.012, 0.140, 0.194, 3.71 and 0.269 mg / kg, respectively. According to the wheat varieties, the Kırik was superior in terms of Cu, Fe, Se, Zn, Co and Cr, and the Dogu-88 was superior in terms of Mn, Cd, and Ni. The effect of weed control methods on mineral content was variable. According to fertilizer sources, the highest mineral content was obtained from the control plots without fertilizer treatments. The lowest mineral contents were obtained from chemical fertilization, cattle manure and organic fertilizer applications. There was no significant increase in the mineral content of wheat with organic fertilization, however, organic agriculture still preserves its place in terms of healthy food. As a result, it has been determined that the values obtained for all mineral elements were not at a level that pose a risk on the environment, human and animal health according to WHO.

Dietary supplements based on modified and enriched with amino acids zeolite in the cultivation of young turkeys

Relevance. Modern turkey crosses have a genetically determined high growth rate. These farm birds are very sensitive to even minor fluctuations in their diet, are susceptible to a deficiency of amino acids, protein, mineral elements and vitamins, which can contribute to a decrease in their productivity, the development of pathological processes and diseases. The use of modern technologies and scientific achievements has made it possible to prepare a new product — modified zeolite. This natural mineral is already free of impurities and microbes, with open pore windows, has a high ability to selectively exchange, and is obtained by various methods of activating zeolite in industrial conditions. At the same time, Ulyanovsk specialists have mastered the technology of enriching modified zeolite with useful substances.Methods. The aim of the work was to study the effect on the body of turkeys and the level of their productivity of additives based on modified zeolite enriched with amino acids. The experiment was organized for 60 days on 250 turkeys of the breed Hybrid Cread-mayker aged 55–60 days in the conditions of the Ulyanovsk region. The control group received the main ration (MR), the experimental group was given once a day in addition to the MR in the morning feeding in a mixture with mixed feed 5% additives of modified zeolite enriched with amino acids.Results. The inclusion of a supplement based on modified zeolite enriched with amino acids in the diet of turkeys does not have a negative effect on the hematological parameters of their blood, all indicators corresponded to the physiological norm. During the experiment in the 2nd group, the live weight of poultry was obtained larger by 8.70%, which amounted to 12,296 kg, respectively, more profit was obtained from the sale of turkey meat, total revenue was 133,210, and additional profit was 27,960 rubles, 2.41 rubles of profit was received for 1 ruble of costs. Тhe data indicate the normalization of mineral homeostasis in the body of turkeys and better absorption of nutrients in the feed diet.

Humik Asit Uygulamalarının Zambak Bitkisinin Gelişimi, Bitkideki Fenolojik ve Pomolojik Gözlemler Üzerine Etkisi

In this study, a pot study was carried out to reveal the effects of direct and foliar applications of humic acid to the growing medium in addition to basic fertilization on the development and plant quality characteristics of lily plants grown in peat + perlite medium. The experiment was carried out in an unheated greenhouse during the summer, in a greenhouse environment with a shade net on it. In the experiment, in addition to the control, the humic acid doses were 3.5 lt/da from soil, 7.5 lt/da from soil, 1.75 lt/da from leaf, 3.75 lt/da from leaf and soil + 3.5 lt/da from leaf + 1, It was applied 3 times as 75 lt/da. In the pot experiment established in three replications according to the randomized plots trial design, 10 lily bulbs were planted in each pot and humic acid applications were made at two-week intervals following plant emergence. In order to see the effect of the applications when the plant is harvested, the length of the flower stem (mm), the thickness of the flower stem (mm), the fresh weight of the branch (mm), the number of buds (pieces), the bud length (cm), the number of leaves (pieces), Full bloom stem (mm) and vase life (days) were examined. As the doses of humic acid increased, the vegetative weight of the lily plant increased, while the effect of the applications on the investigated properties was different. With the mineral elements, hormones and plant growth regulators in humic acid, it has the feature of promoting the increase of biomass in the plant.

Mineral Composition in Delactosed Dairy Products: Quality and Safety Status

Mineral elements are ingested through the diet (Li, Be, B, Na, Mg, Al, K, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Sr, Mo, Ag, Cd, Sb, Ba, Tl, Pb, and Bi). Essential minerals have structural, biochemical, nutritional and catalytic functions; therefore, they are fundamental for human health. In this research, thirty commercial delactosed dairy products from different varieties were supplied by various markets in Sicily (Italy), and their mineral contents were determined by using inductively coupled plasma mass spectrometry (ICP-MS) with the following aims: (1) to highlight the differences among various products; (2) to evaluate if it is possibly related to the analyzed samples of their product group; (3) to evaluate the nutritional quality and safety related to intake of these dairy products. Evident differences were found among the samples depending on the type of product. A good separation between mozzarella—on the one hand—and crescenza and primo sale—on the other—was observed. The mozzarella samples were distinguished by the higher Fe, V and Co contents, and the lower amount of Al. Based on shares of the RDA, the analyzed dairy samples are a good source of Ca (up to 58% of the nutrient reference values), with a relatively high concentration of Na (between 5.5% and 22%). Any safety risk for consumers due to exposures to toxic elements through analyzed samples is excluded. The obtained results give reason to expect further insight concerning the direct comparison between the delactosed and non-delactosed product, in order to evaluate if the manufacturing process can affect the content of some mineral.

Evaluation of Muscle Chemical Composition, Amino Acids Profile and Antioxidative Capacity of the Shaziling Pig and its Crossbreeds

Background: Meat quality in pigs is an extremely important economic trait. The Shaziling pig is representative of good meat quality but has been scarcely utilized because of the unpleasant growth rate and lean percentage. Methods: The differences of muscle chemical composition, amino acids profile and antioxidative capacity were evaluated among [(Berkshire × Shaziling) × (Berkshire × Shaziling) (BS × (B × S)], (Berkshire × Shaziling) × Shaziling [(B × S) × S], Shaziling × (Berkshire × Shaziling) [S × (B × S)] and Shaziling (S × S) pigs. Result: Four groups had plentiful contents of mineral elements (Ca, Zn, Na, K, Cu, Mg, Mn) and abundant amino acids content and no obvious differences in diameters of myofibers and adipocytes were found among four groups. In addition, (B × S) × S pigs had the highest crude protein content, possessed comprehensive nutrient in amino acids and showed preferable antioxidative capacity, suggesting that the meat of (B × S) × S pigs has optimal shelf life.

Composition of Zingiber officinale Roscoe (Ginger), Soil Properties and Soil Enzyme Activities Grown in Different Concentration of Mineral Fertilizers

Ginger is rich in different chemical compounds such as phenolic compounds, terpenes, polysaccharides, lipids, organic acids, minerals, and vitamins. The present study investigated the effect of mineral fertilizers on the content of mineral elements in the rhizomes of Zingiber officinale Roscoe, soil enzymes activity, and soil properties in Surkhandarya Region, Uzbekistan. To the best of our knowledge, the present study is the first in Uzbekistan to investigate the mineral elements of ginger rhizome inhabiting Termez district, Surkhandarya region. A Field experiment was conducted at the Surkhandarya experimental station research Institute. Four treatments have been studied (Control with no fertilizers (T-1), N75P50K50 kg/ha (T-2), N125P100K100 kg/ha (T-3) and N100P75K75 + B3Zn6Fe6 kg/ha (T-4)). Results showed that T-4 treatment significantly increased ginger rhizome K, Ca, P, Mg, Fe, Na, Mn, Zn, Si, Li, and V content as compared to all other treatments and control. T-3 treatment significantly increased Mo, Ga, and Ag content in comparison to other treatments. Soil enzymes showed a significant increase for all treatments against control, while T-4 treatment has recorded the highest enzyme activity in comparison to all other treatments in urease, invertase, and catalase content. Soil chemical properties have significantly changed for all treatments against the non-cultivated soil and the zero fertilizers plantation with variation among different treatments. Results showed that ginger root is rich in minerals and can be used as a great potential for nutritional supplements and soil enrichment. This study suggest that combination of macro-microelements have the potential to increase the content of mineral elements in the rhizomes of ginger in field conditions.

Nutraceutical Properties in Flowers

Flowers are associated with human life since time immemorial. Beside aesthetic beauty, several flowers are used for edible purpose viz., rose, marigold, calendula, hibiscus, hollyhock, carnation, chrysanthemum, nasturtium, lotus, cosmos and pansies etc. These flowers have medicinal as well as nutritional values and are rich source of vitamins, antioxidants and mineral elements. The flowers are enriched with various pigments viz., chlorophyll, flavonoids, xanthophylls, carotenoids, betalains and anthocyanin that are generally used in coloring food, beverage, and textile and paper industries. Edible flowers have anticancer, anti-inflammatory, antitumor, antimicrobial and antimutagenic properties too. The flowers are used as vegetables and other purpose since time immemorial, however, flowers for supplementary health products is comparatively a new concept and therefore needs further research to utilize flowers as nutraceuticals.