Image-Guided Hydrodynamic Gene Delivery to the Liver: Toward Clinical Applications

2016 ◽  
pp. 85-92
Author(s):  
Kenya Kamimura ◽  
Takeshi Suda ◽  
Tsutomu Kanefuji ◽  
Takeshi Yokoo ◽  
Hiroyuki Abe ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Clinical Applications ◽  
Image Guided

scholarly journals Image-Guided Hydrodynamic Gene Delivery: Current Status and Future Directions

Pharmaceutics ◽  
2015 ◽  
Vol 7 (3) ◽  
pp. 213-223 ◽  
Author(s):  
Kenya Kamimura ◽  
Takeshi Yokoo ◽  
Hiroyuki Abe ◽  
Yuji Kobayashi ◽  
Kohei Ogawa ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Current Status ◽  
Future Directions ◽  
Image Guided

Nonviral Vehicles for Gene Delivery

Nano LIFE ◽  
2021 ◽  
Vol 11 (02) ◽  
pp. 2130002
Author(s):  
Eric Warga ◽  
Brian Austin-Carter ◽  
Noelle Comolli ◽  
Jacob Elmer
Keyword(s):  
Gene Delivery ◽  
Lower Cost ◽  
Clinical Applications ◽  
Inorganic Nanoparticles ◽  
Nonviral Gene Delivery ◽  
Viral Gene ◽  
Cationic Polymers ◽  
Recent Developments ◽  
Low Efficiency ◽  
Viral Gene Delivery

Nonviral gene delivery (NVGD) is an appealing alternative to viral gene delivery for clinical applications due to its lower cost and increased safety. A variety of promising nonviral vectors are under development, including cationic polymers, lipids, lipid-polymer hybrids (LPHs) and inorganic nanoparticles. However, some NVGD strategies have disadvantages that have limited their adoption, including high toxicity and low efficiency. This review focuses on the most common NVGD vehicles with an emphasis on recent developments in the field.

scholarly journals 267. Image-Guided, Liver-Targeted Hydrodynamic Gene Delivery in Dogs – Preclinical Assessment of Effectiveness and Safety of the Procedure

2015 ◽  
Vol 23 ◽  
pp. S106-S107
Author(s):  
Kenya Kamimura ◽  
Hiroyuki Abe ◽  
Takeshi Yokoo ◽  
Tsutomu Kanefuji ◽  
Takeshi Suda ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Image Guided

scholarly journals A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain

Gene Therapy ◽  
2011 ◽  
Vol 19 (4) ◽  
pp. 396-403 ◽  
Author(s):  
J Jang ◽  
K Yoon ◽  
D W Hwang ◽  
D S Lee ◽  
S Kim
Keyword(s):  
Gene Delivery ◽  
Mouse Brain ◽  
Ultrasound Image ◽  
Retroviral Vector ◽  
Image Guided

scholarly journals Non-Viral Delivery of RNA Gene Therapy to the Central Nervous System

Pharmaceutics ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 165
Author(s):  
Ellen S. Hauck ◽  
James G. Hecker
Keyword(s):  
Gene Therapy ◽  
Central Nervous System ◽  
Nervous System ◽  
Nucleic Acid ◽  
Gene Delivery ◽  
Dna Delivery ◽  
Clinical Applications ◽  
Cationic Lipids ◽  
Viral Dna ◽  
The Central Nervous System

Appropriate gene delivery systems are essential for successful gene therapy in clinical medicine. Lipid-mediated nucleic acid delivery is an alternative to viral vector-mediated gene delivery and has the following advantages. Lipid-mediated delivery of DNA or mRNA is usually more rapid than viral-mediated delivery, offers a larger payload, and has a nearly zero risk of incorporation. Lipid-mediated delivery of DNA or RNA is therefore preferable to viral DNA delivery in those clinical applications that do not require long-term expression for chronic conditions. Delivery of RNA may be preferable to non-viral DNA delivery in some clinical applications, since transit across the nuclear membrane is not necessary, and onset of expression with RNA is therefore even faster than with DNA, although both are faster than most viral vectors. Delivery of RNA to target organ(s) has previously been challenging due to RNA’s rapid degradation in biological systems, but cationic lipids complexed with RNA, as well as lipid nanoparticles (LNPs), have allowed for delivery and expression of the complexed RNA both in vitro and in vivo. This review will focus on the non-viral lipid-mediated delivery of RNAs, including mRNA, siRNA, shRNA, and microRNA, to the central nervous system (CNS), an organ with at least two unique challenges. The CNS contains a large number of slowly dividing or non-dividing cell types and is protected by the blood brain barrier (BBB). In non-dividing cells, RNA-lipid complexes demonstrated increased transfection efficiency relative to DNA transfection. The efficiency, timing of the onset, and duration of expression after transfection may determine which nucleic acid is best for which proposed therapy. Expression can be seen as soon as 1 h after RNA delivery, but duration of expression has been limited to 5–7 h. In contrast, transfection with a DNA lipoplex demonstrates protein expression within 5 h and lasts as long as several weeks after transfection.

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