Structure, Biological Activity and Environmental Fate of Insecticidal Bt (Bacillus thuringiensis) Cry Proteins of Bacterial and Genetically Modified Plant Origin

2012 ◽  
pp. 49-77 ◽  
Author(s):  
Nordine Helassa ◽  
Hervé Quiquampoix ◽  
Siobhán Staunton
Keyword(s):  
Biological Activity ◽  
Bacillus Thuringiensis ◽  
Environmental Fate ◽  
Genetically Modified ◽  
Plant Origin ◽  
Cry Proteins ◽  
Genetically Modified Plant

Consequences for Protaphorura armata (Collembola: Onychiuridae) following exposure to genetically modified Bacillus thuringiensis (Bt) maize and non-Bt maize

2006 ◽  
Vol 142 (2) ◽  
pp. 212-216 ◽  
Author(s):  
Lars-Henrik Heckmann ◽  
Bryan S. Griffiths ◽  
Sandra Caul ◽  
Jacqueline Thompson ◽  
Marianne Pusztai-Carey ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Genetically Modified ◽  
Bt Maize ◽  
Protaphorura Armata

scholarly journals New Strategy for Identification of Novel cry-Type Genes from Bacillus thuringiensis Strains

2005 ◽  
Vol 71 (2) ◽  
pp. 761-765 ◽  
Author(s):  
Corina M. Berón ◽  
Leonardo Curatti ◽  
Graciela L. Salerno
Keyword(s):  
Bacillus Thuringiensis ◽  
Phylogenetic Distance ◽  
Cloning And Sequencing ◽  
Degenerate Primers ◽  
Dna Templates ◽  
Cry Proteins ◽  
New Strategy ◽  
Genes Encoding ◽  
Encoding Protein

ABSTRACT We designed five degenerate primers for detection of novel cry genes from Bacillus thuringiensis strains. An efficient strategy was developed based on a two-step PCR approach with these primers in five pair combinations. In the first step, only one of the primer pairs is used in the PCR, which allows amplification of DNA fragments encoding protein regions that include consensus domains of representative proteins belonging to different Cry groups. A second PCR is performed by using the first-step amplification products as DNA templates and the set of five primer combinations. Cloning and sequencing of the last-step amplicons allow both the identification of known cry genes encoding Cry proteins covering a wide phylogenetic distance and the detection and characterization of cry-related sequences from novel B. thuringiensis isolates.

scholarly journals Guidance for the risk assessment of the presence at low level of genetically modified plant material in imported food and feed under Regulation (EC) No 1829/2003

EFSA Journal ◽  
2017 ◽  
Vol 15 (11) ◽  
Author(s):  
◽  
Hanspeter Naegeli ◽  
Andrew Nicholas Birch ◽  
Josep Casacuberta ◽  
Adinda De Schrijver ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Plant Material ◽  
Genetically Modified ◽  
Low Level ◽  
Genetically Modified Plant ◽  
Food And Feed ◽  
Imported Food

scholarly journals Selection and Characterization of Bacillus thuringiensis Strains with Biological Activity against Lycoriella ingenua

2018 ◽  
Vol 63 (2) ◽  
pp. 293-302
Author(s):  
You Kyoung LEE ◽  
Na Young JIN ◽  
Hee Ji KIM ◽  
Yong Man YOUN ◽  
Chisa YASUNAGA–AOKI ◽  
...  
Keyword(s):  
Biological Activity ◽  
Bacillus Thuringiensis

Biological Activity of Bacillus thuringiensis (Bacillales: Bacillaceae) in Anastrepha fraterculus (Diptera: Tephritidae)

2018 ◽  
Vol 111 (3) ◽  
pp. 1486-1489 ◽  
Author(s):  
Liliane Nachtigall Martins ◽  
Ana Paula de Souza Stori de Lara ◽  
Márcio Soares Ferreira ◽  
Adrise Medeiros Nunes ◽  
Daniel Bernardi ◽  
...  
Keyword(s):  
Biological Activity ◽  
Bacillus Thuringiensis ◽  
Anastrepha Fraterculus

Characterization of cry Genes in a Mexican Bacillus thuringiensis Strain Collection

1998 ◽  
Vol 64 (12) ◽  
pp. 4965-4972 ◽  
Author(s):  
Alejandra Bravo ◽  
Sergio Sarabia ◽  
Lorena Lopez ◽  
Hernesto Ontiveros ◽  
Carolina Abarca ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Specific Primers ◽  
Cry Proteins ◽  
Pcr Product ◽  
Strain Collection ◽  
Lepidopteran Insects ◽  
Ecological Patterns ◽  
Biogeographical Regions ◽  
Selection Of

ABSTRACT Mexico is located in a transition zone between the Nearctic and Neotropical biogeographical regions and contains a rich and unique biodiversity. A total of 496 Bacillus thuringiensis strains were isolated from 503 soil samples collected from the five macroregions of the country. The characterization of the strain collection provided useful information on the ecological patterns of distribution of B. thuringiensis and opportunities for the selection of strains to develop novel bioinsecticidal products. The analysis of the strains was based on multiplex PCR with novel general and specific primers that could detect the cry1,cry3, cry5, cry7, cry8,cry9, cry11, cry12,cry13, cry14, cry21, andcyt genes. The proteins belonging to the Cry1 and Cry9 groups are toxic for lepidopteran insects. The Cry3, Cry7, and Cry8 proteins are active against coleopteran insects. The Cry5, Cry12, Cry13, and Cry14 proteins are nematocidal. The Cry11, Cry21, and Cyt proteins are toxic for dipteran insects. Six pairs of general primers are used in this method. Strains for which unique PCR product profiles were obtained with the general primers were further characterized by additional PCRs with specific primers. Strains containingcry1 genes were the most abundant in our collection (49.5%). Thirty-three different cry1-type profiles were identified. B. thuringiensis strains harboringcry3 genes represented 21.5% of the strains, and 7.9% of the strains contained cry11 and cyt genes.cry7, cry8, and cry9 genes were found in 0.6, 2.4, and 2.6% of the strains, respectively. No strains carrying cry5, cry12, cry13,cry14, or cry21 genes were found. Finally, 14% of the strains did not give any PCR product and did not react with any polyclonal antisera. Our results indicate the presence of strains that may harbor potentially novel Cry proteins as well as strains with combinations of less frequently observed cry genes.

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