The epidemiology of high density lipoproteins: The Lipid Research Clinics Program Prevalence Study

1982 ◽  
pp. 61-75
Author(s):  
B. M. Rifkind ◽  
G. Heiss ◽  
H. A. Tyroler ◽  
O. D. Williams
Keyword(s):  
High Density ◽  
Prevalence Study ◽  
High Density Lipoproteins ◽  
Lipid Research

Enzymatic determinations of cholesterol in high-density-lipoprotein fractions prepared by a precipitation technique.

1976 ◽  
Vol 22 (1) ◽  
pp. 98-101 ◽  
Author(s):  
B W Steele ◽  
D F Koehler ◽  
M M Azar ◽  
T P Blaszkowski ◽  
K Kuba ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Total Cholesterol ◽  
Convenient Method ◽  
Density Lipoprotein ◽  
High Density ◽  
High Density Lipoproteins ◽  
Lipid Research ◽  
Enzymatic Method ◽  
Fine Precipitate

Abstract An enzymatic method for cholesterol in serum [Clin. Chem. 20, 470 (1974)] was initially found to be unsatisfactory for measuring cholesterol in high-density-lipoprotein fractions prepared by precipitation with Mn2+. A fine precipitate formed in the cuvette and cholesterol values were falsely increased. We describe a simple, convenient method for circumventing these problems. An ethylenediaminetetraacetate solution is used to reconstitute the enzymatic reagent. Cholesterol values by this procedure correlated with those obtained by the Lipid Research Clinic's procedure for the same lipoprotein fraction preparations (regression slope, .998; Y-intercept, 8.9 mg/liter; correlation coefficient, .984; standard error of the estimate, 16.8 mg/liter). Precision of the assay, including the precipitation step, was calculated. The SDwithin day was 9.7 mg/liter and SDoverall was 23.7 mg/liter. Results for total cholesterol with the modified reagent were linearly related to concentrations exceeding 4 g/liter, thereby permitting determination of high-density-lipoproteins and total cholesterol in a single run.

scholarly journals Importance of Apolipoprotein A-I and A-II Composition in HDL and Its Potential for Studying COVID-19 and SARS-CoV-2

Medicines ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 38
Author(s):  
Kyung-Hyun Cho
Keyword(s):  
Antiviral Activity ◽  
High Density ◽  
High Density Lipoproteins ◽  
Potent Antiviral Activity ◽  
Apolipoprotein A ◽  
Putative Role

The composition and properties of apolipoprotein (apo) A-I and apoA-II in high-density lipoproteins (HDL) might be critical to SARS-CoV-2 infection via SR-BI and antiviral activity against COVID-19. HDL containing native apoA-I showed potent antiviral activity, while HDL containing glycated apoA-I or other apolipoproteins did not. However, there has been no report to elucidate the putative role of apoA-II in the antiviral activity of HDL.

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