Adhesion of Escherichia Coli Pathogenic in Pigs, Calves and Lambs to Intestinal Epithelial Cell Brush-Borders

1981 ◽  
pp. 163-170
Author(s):  
R. Sellwood ◽  
D. Lees
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Brush Borders

Enterotoxigenic Escherichia coli infection induces intestinal epithelial cell autophagy

2014 ◽  
Vol 171 (1-2) ◽  
pp. 160-164 ◽  
Author(s):  
Yulong Tang ◽  
Fengna Li ◽  
Bie Tan ◽  
Gang Liu ◽  
Xiangfeng Kong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Epithelial ◽  
Escherichia Coli Infection

scholarly journals Reactivation of intestinal epithelial cell brush border motility: ATP-dependent contraction via a terminal web contractile ring.

1982 ◽  
Vol 95 (3) ◽  
pp. 853-863 ◽  
Author(s):  
D R Burgess
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cell ◽  
Brush Border ◽  
Intestinal Epithelial Cell ◽  
Intercellular Junctions ◽  
Intestinal Epithelial ◽  
Differential Interference Contrast Microscopy ◽  
Contractile Ring ◽  
Terminal Web ◽  
Brush Borders

Various models have been put forward suggesting ways in which brush borders from intestinal epithelial cells may be motile. Experiments documenting putative brush border motility have been performed on isolated brush borders and have generated models suggesting microvillar retraction or microvillar rootlet interactions. The reported Ca++ ATP-induced retraction of microvilli has been shown, instead, to be microvillar dissolution in response to Ca++ and not active brush border motility. I report here studies on the reactivation of motility in intact sheets of isolated intestinal epithelium. Whole epithelial sheets were glycerinated, which leaves the brush border and intercellular junctions intact, and then treated with ATP, PPi, ITP, ADP, GTP, or delta S-ATP. Analysis by video enhanced differential interference-contrast microscopy and thin-section transmission electron microscopy reveals contractions in the terminal web region causing microvilli to be fanned apart in response to ATP and delta S-ATP but not in response to ADP, PPi, ITP, or GTP. Electron microscopy reveals that the contractions occur at the level of the intermediate junction in a circumferential constriction which can pull cells completely apart. This constriction occurs in a location occupied by an actin-containing circumferential band of filaments, as demonstrated by S-1 binding, which completely encircles the terminal web at the level of the intermediate junction. Upon contraction, this band becomes denser and thicker. Since myosin, alpha-actinin and tropomyosin, in addition to actin, have been localized to this region of the terminal web, it is proposed that the intestinal epithelial cell can be motile via a circumferential terminal web contractile ring analogous to the contractile ring of dividing cells.

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