Survival of Cultured and Transported Bovine Embryos Following Surgical and Non-Surgical Transfers

1978 ◽  
pp. 356-362 ◽  
Author(s):  
J. Hahn ◽  
L. A. Moustafa ◽  
U. Schneider ◽  
R. Hahn ◽  
W. Romanowski ◽  
...  
Keyword(s):  
Bovine Embryos

Early development of bovine embryos depends on the cooperative action between oxidative and endoplasmic reticulum stresses

2014 ◽  
Author(s):  
Seung-Bin Yoon ◽  
Hae-Jun Yang ◽  
Seong-Eun Mun ◽  
Pil-Soo Jeong ◽  
Seon-A Choi ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Early Development ◽  
Bovine Embryos ◽  
Cooperative Action

scholarly journals Evaluation of Bovine Embryo Biopsy Techniques according to Their Ability to Preserve Embryo Viability

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
M. Cenariu ◽  
E. Pall ◽  
C. Cernea ◽  
I. Groza
Keyword(s):  
Pregnancy Rate ◽  
Genetic Diagnosis ◽  
Needle Aspiration ◽  
Embryo Biopsy ◽  
Embryo Cryopreservation ◽  
Bovine Embryo ◽  
Bovine Embryos ◽  
Embryo Viability ◽  
Biopsy Methods ◽  
Biopsy Method

The purpose of this research was to evaluate three embryo biopsy techniques used for preimplantation genetic diagnosis (PGD) in cattle and to recommend the least invasive one for current use, especially when PGD is followed by embryo cryopreservation. Three hundred bovine embryos were biopsied by either one of the needle, aspiration or microblade method, and then checked for viability by freezing/thawing and transplantation to recipient cows. The number of pregnancies obtained after the transfer of biopsied frozen/thawed embryos was assessed 30 days later using ultrasounds. The results were significantly different between the three biopsy methods: the pregnancy rate was of 57% in cows that received embryos biopsied by needle, 43% in cows that received embryos biopsied by aspiration, and 31% in cows that received embryos biopsied by microblade. Choosing an adequate biopsy method is therefore of great importance in embryos that will undergo subsequent cryopreservation, as it significantly influences their viability after thawing.

scholarly journals Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos

2019 ◽  
Vol 54 (10) ◽  
pp. 1357-1365
Author(s):  
Luiz Sergio Almeida Camargo ◽  
Fernanda Queiros Costa ◽  
Michele Munk ◽  
Sabine Wohlres‐Viana ◽  
Raquel Varela Serapião ◽  
...  
Keyword(s):  
Heat Shock ◽  
In Vitro Maturation ◽  
Bovine Embryos

scholarly journals Microinjection of Bovine Embryos with a Foreign Gene and Its Detection at the Blastocyst Stage

1993 ◽  
Vol 76 (11) ◽  
pp. 3392-3399 ◽  
Author(s):  
Esmail Behboodi ◽  
G.B. Anderson ◽  
S. Horvat ◽  
J.F. Medrano ◽  
J.D. Murray ◽  
...  
Keyword(s):  
Blastocyst Stage ◽  
Foreign Gene ◽  
Bovine Embryos

scholarly journals Sex Determination of Bovine Embryos Using H-Y Antibodies

1989 ◽  
Vol 30 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Birthe Avery ◽  
Mette Schmidt
Keyword(s):  
Sex Determination ◽  
Bovine Embryos

scholarly journals Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karina Cañón-Beltrán ◽  
Yulia N. Cajas ◽  
Serafín Peréz-Cerezales ◽  
Claudia L. V. Leal ◽  
Ekaitz Agirregoitia ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Mitochondrial Activity ◽  
Bovine Embryos ◽  
Akt Signaling Pathway ◽  
Cell Stage ◽  
Development In Vitro

AbstractIn vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.

scholarly journals Sex Determination of Bovine Embryos Based on Embryonic Cleavage Rates

1989 ◽  
Vol 30 (2) ◽  
pp. 147-153
Author(s):  
Birthe Avery ◽  
Mette Schmidt ◽  
Torben Greve
Keyword(s):  
Sex Determination ◽  
Bovine Embryos ◽  
Embryonic Cleavage

Development of bovine embryos on Vero/BRL cell monolayers (mixed co-culture)

2000 ◽  
Vol 54 (8) ◽  
pp. 1239-1247 ◽  
Author(s):  
A.M. Duszewska ◽  
Z. Reklewski ◽  
M. Pieńkowski ◽  
J. Karasiewicz ◽  
J.A. Modliński
Keyword(s):  
Bovine Embryos ◽  
Cell Monolayers
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