Mechanism of Glucocorticoid Hormone Action and of Regulation of Gene Expression in Cultured Mammalian Cells

1973 ◽  
pp. 206-224 ◽  
Author(s):  
John D. Baxter ◽  
Guy G. Rousseau ◽  
Stephen J. Higgins ◽  
Gordon M. Tomkins
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Regulation Of Gene Expression ◽  
Hormone Action ◽  
Glucocorticoid Hormone

scholarly journals A more efficient RNAi inducible system for tight regulation of gene expression in mammalian cells and xenograft animals

RNA ◽  
2007 ◽  
Vol 13 (8) ◽  
pp. 1375-1383 ◽  
Author(s):  
J. Zhang ◽  
C. Wang ◽  
N. Ke ◽  
J. Bliesath ◽  
J. Chionis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Regulation Of Gene Expression ◽  
Expression In Mammalian Cells

Regulation of gene expression and cellular proliferation by histone H2A.ZThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB’s 51st Annual Meeting – Epigenetics and Chromatin Dynamics, and has undergone the Journal’s usual peer review process.

2009 ◽  
Vol 87 (1) ◽  
pp. 179-188 ◽  
Author(s):  
Amy Svotelis ◽  
Nicolas Gévry ◽  
Luc Gaudreau
Keyword(s):  
Gene Expression ◽  
Transcriptional Activation ◽  
Mammalian Cells ◽  
Cellular Proliferation ◽  
Replicative Senescence ◽  
Peer Review Process ◽  
Regulation Of Gene Expression ◽  
Histone Variants ◽  
Chromatin Dynamics ◽  
Cycle Arrest

The mammalian genome is organized into a structure of DNA and proteins known as chromatin. In general, chromatin presents a barrier to gene expression that is regulated by several pathways, namely by the incorporation of histone variants into the nucleosome. In yeast, H2A.Z is an H2A histone variant that is incorporated into nucleosomes as an H2A.Z/H2B dimer by the Swr1 complex and by the SRCAP and p400/Tip60 complexes in mammalian cells. H2A.Z has been associated with the poising of genes for transcriptional activation in the yeast model system, and is essential for development in higher eukaryotes. Recent studies in our laboratory have demonstrated a p400-dependent deposition of H2A.Z at the promoter of p21WAF1/CIP1, a consequence that prevents the activation of the gene by p53, thereby inhibiting p53-dependent replicative senescence, a form of cell-cycle arrest crucial in the prevention of carcinogenic transformation of cells. Moreover, H2A.Z is overexpressed in several different types of cancers, and its overexpression has been associated functionally with the proliferation state of cells. Therefore, we suggest that H2A.Z is an important regulator of gene expression, and its deregulation may lead to the increased proliferation of mammalian cells.

scholarly journals Selective Translation Complex Profiling Reveals Staged Initiation and Co-translational Assembly of Initiation Factor Complexes

2019 ◽  
Author(s):  
Susan Wagner ◽  
Anna Herrmannová ◽  
Vladislava Hronová ◽  
Neelam Sen ◽  
Ross D. Hannan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Translational Control ◽  
Mammalian Cells ◽  
Regulation Of Gene Expression ◽  
Ribosome Profiling ◽  
Initiation Factor ◽  
Start Codon ◽  
Codon Recognition ◽  
Initiation Phase ◽  
Gene Expression Studies

SUMMARYTranslational control targeting mainly the initiation phase is central to the regulation of gene expression. Understanding all of its aspects requires substantial technological advancements. Here we modified yeast Translational Complex Profile sequencing (TCP-seq), related to ribosome profiling, and adopted it for mammalian cells. Human TCP-seq, capable of capturing footprints of 40S subunits (40Ses) in addition to 80S ribosomes (80Ses), revealed that mammalian and yeast 40Ses distribute similarly across 5’UTRs indicating considerable evolutionary conservation. We further developed a variation called Selective TCP-seq (Sel-TCP-seq) enabling selection for 40Ses and 80Ses associated with an immuno-targeted factor in yeast and human. Sel-TCP-seq demonstrated that eIF2 and eIF3 travel along 5’UTRs with scanning 40Ses to successively dissociate upon start codon recognition. Manifesting the Sel-TCP-seq versatility for gene expression studies, we also identified four initiating 48S conformational intermediates, provided novel insights into ATF4 and GCN4 mRNA translational control, and demonstrated co-translational assembly of initiation factor complexes.

Negative and positive regulation in trans of gene expression from adeno-associated virus vectors in mammalian cells by a viral rep gene product

1986 ◽  
Vol 6 (8) ◽  
pp. 2884-2894 ◽  
Author(s):  
J D Tratschin ◽  
J Tal ◽  
B J Carter
Keyword(s):  
Gene Expression ◽  
Gene Product ◽  
Mammalian Cells ◽  
Regulation Of Gene Expression ◽  
Negative Regulator ◽  
Positive Regulation ◽  
Aav Vector ◽  
Adeno Associated Virus ◽  
293 Cells ◽  
In Trans

We previously described use of the human parvovirus, adeno-associated virus (AAV), as a vector for transient expression in mammalian cells of the gene for chloramphenicol acetyltransferase (CAT). In the AAV vector, pTS1, the CAT gene is expressed under the control of the major AAV promoter p40. This promoter is embedded within the carboxyl-terminal region of an open reading frame (orf-1) which codes for a protein (rep) required for AAV DNA replication. We show here that the rep product has additional trans-acting properties to regulate gene expression. First, deletion or frame-shift mutations in orf-1, which occurred far upstream of p40, increased expression of CAT in human 293 (adenovirus-transformed) cells. This increased CAT expression was abolished when such mutant AAV vectors were transfected into 293 cells together with a second AAV vector which could supply the wild-type AAV rep product in trans. Thus, an AAV rep gene product was a negative regulator, in trans, of expression of CAT in uninfected 293 cells. In adenovirus-infected 293 cells, the function of the AAV rep product was more complex, but in some cases, it appeared to be a trans activator of the expression from p40. In HeLa cells, only trans activation by rep was seen in the absence or presence of adenovirus. Neither activation nor repression by the rep product required replication per se of the AAV vector DNA. Thus, trans-acting negative or positive regulation of gene expression by the AAV rep gene is modulated by factors in the host cell and by the helper adenovirus.

An engineered PGK promoter and lac operator-repressor system for the regulation of gene expression in mammalian cells

Gene ◽  
1993 ◽  
Vol 130 (2) ◽  
pp. 233-239 ◽  
Author(s):  
Garry N. Hannan ◽  
Sigrid A. Lehnert ◽  
Elizabeth S. MacAvoy ◽  
Philip A. Jennings ◽  
Peter L. Molloy
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Regulation Of Gene Expression ◽  
Lac Operator ◽  
Expression In Mammalian Cells

scholarly journals Expression and gene regulation network of EAF2 in cervical cancer based on data mining

2020 ◽  
Author(s):  
Wei na Kong ◽  
Fan Guo ◽  
Yang chun Feng ◽  
Jie Lv ◽  
Jia hui Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cervical Cancer ◽  
Cancer Progression ◽  
Survival Data ◽  
Mammalian Cells ◽  
Gene Networks ◽  
Patient Survival ◽  
Regulation Of Gene Expression ◽  
Cervical Cancer Patient ◽  
Risk Of Cancer

Abstract Background: ELL-associated factor 2 (EAF2) plays an important role in transcription elongation and the regulation of gene expression in both mammalian cells as well as in lower eukaryotes concurrent . EAF2’s depletion has been demonstrated to enhance cell proliferation and greatly increase the risk of cancer. However, little is known about the expression and function of EAF2 in cervical cancer (CC) progression. Here, we comprehensively analyzed the expression of EAF2 and its clinical outcome in CC using publicly available cancer gene expression and patient survival data through various databases.Methods: We examined the differences of EAF2 expression between cancers and their normal tissues using the Oncomine, Gene expression Profiling Interactive Analysis 2 (GEPIA2), the Gene Expression across Normal and Tumor tissue 2 (GENT2) database and UALCAN databases. EAF2 expression was investigated from immunohistochemistry images using the Human Protein Atlas database. Copy number alterations (CNAs) and mutations of EAF2 were analyzed using cBioPortal. Kaplan–Meier analysis was used to predict the survival of EAF2 in CC. Analysis of the co-expression profile of EAF2 and the enrichment pathway of co-expression with EAF2 were revealed using LinkedOmics to explore the predicted signaling pathways. GeneMANIA visualize the gene networks and predict function of genes that GSEA identified as being enriched in CC: kinase LYN, mi-RNA133A, 133B and transcription factor OCT1. Results: We found that the expression of EAF2 decreased with the development of CC and significant upregulation of EAF2 is positively correlated with the overall survival (OS) of CC patients. The decrease of EAF2 gene expression may be partly due to promoter methylation and CNAs with the development of CC. Besides, EAF2 expression might be strongly positively correlated with the expression of IQCB1, ILDR1 and ASTE1, and may contribute to a signaling pathway in CC. Conclusion: Decreased EAF2 expression has negative clinical significance in the development of CC through the regulation of methylation, CNAs and related pathways. This suggests that EAF2 has potential as a therapeutic target for CC. Keywords: EAF2; cervical cancer; patient survival; clinical outcomes; cancer progression; multiomics analysis

scholarly journals Precise tuning of gene expression output levels in mammalian cells

2018 ◽  
Author(s):  
Yale S. Michaels ◽  
Mike B. Barnkob ◽  
Hector Barbosa ◽  
Toni A. Baeumler ◽  
Mary K. Thompson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
High Throughput Sequencing ◽  
Regulation Of Gene Expression ◽  
Antigen Expression ◽  
Control Of Gene Expression ◽  
Precise Control ◽  
Mouse Melanoma Model

ABSTRACTPrecise, analogue regulation of gene expression is critical for development, homeostasis and regeneration in mammals. In contrast, widely employed experimental and therapeutic approaches such as knock-in/out strategies are more suitable for binary control of gene activity, while RNA interference (RNAi) can lead to pervasive off-target effects and unpredictable levels of repression. Here we report on a method for the precise control of gene expression levels in mammalian cells based on engineered, synthetic microRNA response elements (MREs). To develop this system, we established a high-throughput sequencing approach for measuring the efficacy of thousands of miR-17 MRE variants. This allowed us to create a library of microRNA silencing-mediated fine-tuners (miSFITs) of varying strength that can be employed to control the expression of user specified genes. To demonstrate the value of this technology, we used a panel of miSFITs to tune the expression of a peptide antigen in a mouse melanoma model. This analysis revealed that antigen expression level is a key determinant of the anti-tumour immune response in vitro and in vivo. miSFITs are a powerful tool for modulating gene expression output levels with applications in research and cellular engineering.

scholarly journals Cohesin residency determines chromatin loop patterns

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Lorenzo Costantino ◽  
Tsung-Han S Hsieh ◽  
Rebecca Lamothe ◽  
Xavier Darzacq ◽  
Douglas Koshland
Keyword(s):  
Gene Expression ◽  
Chromosome Segregation ◽  
Mammalian Cells ◽  
Regulation Of Gene Expression ◽  
S Phase ◽  
Chromatin Loop ◽  
Wild Type ◽  
Genome Wide ◽  
Mutant Cells ◽  
Order Structures

The organization of chromatin into higher order structures is essential for chromosome segregation, the repair of DNA-damage, and the regulation of gene expression. Using Micro-C XL to detect chromosomal interactions, we observed the pervasive presence of cohesin-dependent loops with defined positions throughout the genome of budding yeast, as seen in mammalian cells. In early S phase, cohesin stably binds to cohesin associated regions (CARs) genome-wide. Subsequently, positioned loops accumulate with CARs at the bases of the loops. Cohesin regulators Wpl1 and Pds5 alter the levels and distribution of cohesin at CARs, changing the pattern of positioned loops. From these observations, we propose that cohesin with loop extrusion activity is stopped by preexisting CAR-bound cohesins, generating positioned loops. The patterns of loops observed in a population of wild-type and mutant cells can be explained by this mechanism, coupled with a heterogeneous residency of cohesin at CARs in individual cells.

Sign in / Sign up

Export Citation Format

Share Document