The effect of carbohydrate source and pH on in vitro growth of Vitis vinifera cultivars Black Hamburg and Alvarino

1994 ◽  
pp. 94-97
Author(s):  
Seán Mac An tSaoir ◽  
Vassilis Damvoglou
Keyword(s):  
Vitis Vinifera ◽  
In Vitro Growth ◽  
Carbohydrate Source

scholarly journals Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

2018 ◽  
Vol 48 (7) ◽  
Author(s):  
Renato Fernandes Galdiano Júnior ◽  
Cibele Mantovani ◽  
Eliana Gertrudes de Macedo Lemos
Keyword(s):  
Survival Data ◽  
Culture Medium ◽  
Seedling Survival ◽  
In Vitro Growth ◽  
Growth Data ◽  
Carbohydrate Source ◽  
Carbohydrate Supplementation ◽  
Percentage Survival ◽  
Half Strength

ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.

Éléments du contrôle de la morphogenèse de la vigne in vitro: sympodisation

1990 ◽  
Vol 68 (4) ◽  
pp. 841-851 ◽  
Author(s):  
Jean-Claude Fournioux ◽  
Roger Bessis
Keyword(s):  
Vitis Vinifera ◽  
Shoot Apex ◽  
Apical Meristem ◽  
Axillary Buds ◽  
Vitis Vinifera L ◽  
In Vitro Growth ◽  
Simultaneous Formation ◽  
Restricted Area

In vitro growth of the grapevine, Vitis vinifera L., may result in the usurping development of the axillary buds at certain nodes and the sympodisation of the shoot. Many theories on the sympodial nature of the branches developped in situ have already been proposed. All these theories seem to be speculative. In this study, the usurpation is not hypothetical. All the morphological facts described here prove its reality. Sympodisation is closely related to tendril formation and occurs only if the young shoots grown in vitro bear tendrils. When present, usurpation is always observed in a node with tendril. Sympodisation is demonstrated by the observation of prefoliar characters and phyllotactic planes, which allow the order of the different planes to be determined. Based on these observations, we formulate a hypothesis to explain this phenomenon. The size of the apical meristem in in vitro conditions being much less than the normal size, a restricted area should remain after the simultaneous formation of a leaf and a tendril. However, if this meristematic residue remains relatively voluminous, the shoot apex may be regenerated. If not, the restoration can not take place and sympodisation will result.

Effect of in Vivo Oxidized Cellulose on in Vitro Growth of Human Respiratory Mucosa and Sub-mucosa During Endoscopic Skull Base Approaches

2016 ◽  
Vol 77 (S 01) ◽  
Author(s):  
Ezequiel Goldschmidt ◽  
Jorge Rasmussen ◽  
Joseph Chabot ◽  
Monica Loressi ◽  
Marcelo Ielpi ◽  
...  
Keyword(s):  
Skull Base ◽  
Oxidized Cellulose ◽  
In Vitro Growth ◽  
Respiratory Mucosa

scholarly journals Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 490
Author(s):  
Martin Sák ◽  
Ivana Dokupilová ◽  
Šarlota Kaňuková ◽  
Michaela Mrkvová ◽  
Daniel Mihálik ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vitis Vinifera ◽  
Cell Cultures ◽  
Trichoderma Viride ◽  
Vitis Vinifera L ◽  
Treated Plant ◽  
Abiotic Elicitors ◽  
In Vitro Cell Cultures ◽  
Red Grape

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

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