scholarly journals Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 490
Author(s):  
Martin Sák ◽  
Ivana Dokupilová ◽  
Šarlota Kaňuková ◽  
Michaela Mrkvová ◽  
Daniel Mihálik ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vitis Vinifera ◽  
Cell Cultures ◽  
Trichoderma Viride ◽  
Vitis Vinifera L ◽  
Treated Plant ◽  
Abiotic Elicitors ◽  
In Vitro Cell Cultures ◽  
Red Grape

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

scholarly journals Elicitation Phenolic Compounds in Cell Culture of Vitis vinifera L. by Phaeomoniella chlamydospora

2014 ◽  
Vol 13 (2) ◽  
pp. 162-171 ◽  
Author(s):  
Martin Sák ◽  
Ivana Dokupilová ◽  
Daniel Mihálik ◽  
Jana Lakatošová ◽  
Marcela Gubišová ◽  
...  
Keyword(s):  
Cell Culture ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Optimal Time ◽  
Vitis Vinifera L ◽  
Total Polyphenols ◽  
Phaeomoniella Chlamydospora ◽  
Esca Disease ◽  
In Vitro Cell Cultures

Abstract The in vitro cell cultures of Vitis vinifera L. cv. St. Laurent were treated with two elicitors - synthetic methyl jasmonate and natural, prepared from grapevine plant infected with the Phaeomoniella chlamydospora, the agent causing the Esca disease of grapevine. Efficiency of phenolic compounds production after elicitation of cell culture was analysed immediately after treatment (15 min, 30 min, 60 min) and later (after 24, 48, and 72 hours). The cell growth and content of phenolic compounds (+)-catechin, (-)-epicatechin, p-coumaric acid, syringaldehyde, rutin, vanillic acid, and trans-resveratrol were analysed in cultivated cells as well as in cultivation medium. Pch-treatment increased production of total polyphenols the most significantly 15 min after the elicitation and in optimal time was 2.86 times higher than in nonelicited culture and 1.44 times higher than in MeJa induced cell culture.

Antioxidant Profile and in Vitro Cardiac Radical-Scavenging versus Pro-oxidant Effects of Commercial Red Grape Juices (Vitis vinifera L. cv. Aglianico N.)

2012 ◽  
Vol 60 (38) ◽  
pp. 9680-9687 ◽  
Author(s):  
Gian Carlo Tenore ◽  
Michele Manfra ◽  
Paola Stiuso ◽  
Luigi Coppola ◽  
Mariateresa Russo ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Radical Scavenging ◽  
Vitis Vinifera L ◽  
Antioxidant Profile ◽  
Red Grape ◽  
Grape Juices

scholarly journals Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv. Syrah grape pomace extract

2019 ◽  
Vol 10 (4) ◽  
pp. 1856-1869 ◽  
Author(s):  
Joana R. Costa ◽  
Manuela Amorim ◽  
Ana Vilas-Boas ◽  
Renata V. Tonon ◽  
Lourdes M. C. Cabral ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Human Health ◽  
Grape Pomace ◽  
Vitis Vinifera L ◽  
Gastrointestinal Digestion ◽  
Gastrointestinal Health ◽  
Bioactive Properties

Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including in gastrointestinal health.

scholarly journals Co-operative action by endo- and exo-β-(1→3)-glucanases from parasitic fungi in the degradation of cell-wall glucans of Sclerotinia sclerotiorum (Lib.) de Bary

1974 ◽  
Vol 140 (1) ◽  
pp. 47-55 ◽  
Author(s):  
David Jones ◽  
Alex. H. Gordon ◽  
John S. D. Bacon
Keyword(s):  
Cell Wall ◽  
Sclerotinia Sclerotiorum ◽  
Culture Filtrate ◽  
Cell Walls ◽  
Trichoderma Viride ◽  
Major Constituent ◽  
Coniothyrium Minitans ◽  
Parasitic Fungi ◽  
Complete Breakdown

1. Two fungi, Coniothyrium minitans Campbell and Trichoderma viride Pers. ex Fr., were grown on autoclaved crushed sclerotia of the species Sclerotinia sclerotiorum, which they parasitize. 2. in vitro the crude culture filtrates would lyse walls isolated from hyphal cells or the inner pseudoparenchymatous cells of the sclerotia, in which a branched β-(1→3)-β-(1→6)-glucan, sclerotan, is a major constituent. 3. Chromatographic fractionation of the enzymes in each culture filtrate revealed the presence of several laminarinases, the most active being an exo-β-(1→3)-glucanase, known from previous studies to attack sclerotan. Acting alone this brought about a limited degradation of the glucan, but the addition of fractions containing an endo-β-(1→3)-glucanase led to almost complete breakdown. A similar synergism between the two enzymes was found in their lytic action on cell walls. 4. When acting alone the endo-β-(1→3)-glucanase had a restricted action, the products including a trisaccharide, tentatively identified as 62-β-glucosyl-laminaribiose. 5. These results are discussed in relation to the structure of the cell walls and of their glucan constituents.

scholarly journals Preliminary Characterization of Wild Grapevine Populations (Vitis vinifera ssp. sylvestris) Grown Along the Danube River

2013 ◽  
Vol 41 (2) ◽  
pp. 472 ◽  
Author(s):  
Carmen F. POPESCU ◽  
Liviu C. DEJEU ◽  
Rafael R. OCETE
Keyword(s):  
Vitis Vinifera ◽  
Morphological Analysis ◽  
Danube River ◽  
Vitis Vinifera L ◽  
Meristematic Tissue ◽  
The Danube River ◽  
Wild Grapevine ◽  
The Moment

The individuals belonging to three different groups of wild grapevines populations Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi harvested along, or near the Danube River, were described by means of usual ampelographic methods. The twenty standardized descriptors used for morphological analysis revealed obvious differentiation among analyzed populations. Out of 65 individuals, a half produced flowers with separate sex and a high proportion of them were males (70%). Pollen measurements on light microscope provided information on differences in pollen size among inside wild grapevine populations of V. sylvestris with the polar length varying between 15.3 and 23 μm and the equatorial length between 15.5 and 24.4μm. The in vitro regenerative potential from meristematic tissue tested with each phenotype showed that the moment of differentiation, the aspect of proliferative structures and the rate of multiplication varied inside these wild grapevine populations, without any correlation with the location of harvesting. Our results provided valuable information about these Vitis vinifera ssp. sylvestris populations, possible to be used as starting plant material for research in general and further breeding of cultivars and grapevine rootstocks.

scholarly journals Factors affecting in vitro cultivation of grape (Vitis vinifera L.): a review

2020 ◽  
Vol 10 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Fikadu Kumsa
Keyword(s):  
Vitis Vinifera ◽  
Review Paper ◽  
Vitis Vinifera L ◽  
Growth Media ◽  
In Vitro Cultivation ◽  
Factors Affecting ◽  
Current Review ◽  
Research Gap ◽  
Grape Varieties

Grape (Vitis vinifera L.) is globally cultivated as commercial fruit crop usually used for fruit purpose or industrial product. The objective of the current review is to review and identify the research gap on the effect of different growth media and vitrification on shooting and rooting performance of grape. Factors affecting rooting of grape cuttings can be internal or external factors. Currently, grapevines are very sensitive to disease in the conventional method of propagation. Even if tissue culture is recommended for healthy propagation of the grape varieties, still factors affecting the growth of the plant verifications were reported. This, review paper progressively revised for the existing factors and possible solutions during in vitro propagation of grapevines. Int. J. Agril. Res. Innov. Tech. 10(1): 1-5, June 2020

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