FT-IR Microspectroscopic Imaging of Human Carcinoma Tissue Thin Sections

1997 ◽  
pp. 441-442
Author(s):  
P. Lasch ◽  
D. Naumann
Keyword(s):  
Human Carcinoma ◽  
Thin Sections ◽  
Carcinoma Tissue ◽  
Ft Ir

High molecular mass type IV collagen-specific metalloprotease from human carcinoma tissue

FEBS Letters ◽  
1988 ◽  
Vol 232 (1) ◽  
pp. 140-144 ◽  
Author(s):  
Michio Tsuda ◽  
Yumi Yamagishi ◽  
Tsunehiko Katsunuma
Keyword(s):  
Molecular Mass ◽  
Type Iv Collagen ◽  
High Molecular Mass ◽  
Human Carcinoma ◽  
Carcinoma Tissue ◽  
Type Iv ◽  
Mass Type

Imaging of human colon carcinoma thin sections by FT-IR microspectrometry

1998 ◽  
Author(s):  
Peter Lasch ◽  
Wolfgang Waesche ◽  
W. J. McCarthy ◽  
Gerhard J. Mueller ◽  
Dieter Naumann
Keyword(s):  
Colon Carcinoma ◽  
Human Colon ◽  
Thin Sections ◽  
Human Colon Carcinoma ◽  
Ft Ir

The Mechanism of the Low-Temperature Oxidation of Coal by O2: Observation and Separation of Simultaneous Reactions Using In Situ FT-IR Difference Spectroscopy

1987 ◽  
Vol 41 (1) ◽  
pp. 50-63 ◽  
Author(s):  
Jon S. Gethner
Keyword(s):  
Low Temperature ◽  
Reactive Species ◽  
Spontaneous Ignition ◽  
Difference Spectroscopy ◽  
Low Temperature Oxidation ◽  
Experimental Conditions ◽  
Temperature Oxidation ◽  
Thin Sections ◽  
Ft Ir

Coal is complex, predominantly organic-containing porous solid which is important both as an energy and a chemical source material. The physical and chemical properties of most coals are extremely sensitive to air oxidation. There is no generally accepted mechanism for the oxidation process, in spite of past interest. Using in situ FT-IR difference spectroscopy of 0.4 μm thin sections of coal, we have examined the mechanism of the low-temperature oxidation of Illinois No. 6 bituminous coal by O2. The overall oxidation with O2 is found to be comprised of three separate chemical reactions. Two of the reactions involve O2 addition to reactive species in the coal. One is predominant at temperatures close to or slightly above room temperature and apparently involves the reversible binding of O2 to a free radical site, followed by reaction. The other oxidation is predominant by 100°C and proceeds by the formation and subsequent decomposition of hydroperoxides. The third reaction is a thermolysis which is important at temperatures between 25°C and 100°C and is competitive with the lower-temperature oxidation. It results in a partial decarbonylation and decarboxylation of the coal. Since three separate reactions contribute to the overall oxidation, the chemical and physical changes resulting from oxidation are dependent upon the oxidation conditions. Control of experimental conditions is critical in order for one to obtain reproducible results. Some of the possible implications of these results on the technologically important process of spontaneous ignition of coal are discussed. Results of previous oxidation studies are discussed in view of the present results. The large variations reported in oxidation studies are likely to be the consequence of ill-defined or poorly controlled experiments. We interpret the correlation between the present study and number of other studies to indicate that oxidation chemistry is the same in most coals, with the principal differences between coals being due to the different relative proportions of the reactive species in the starting coal.

FT-IR microspectroscopic imaging of human melanoma thin sections

1998 ◽  
Author(s):  
P. Lasch ◽  
W. Wäsche ◽  
G. Müller ◽  
D. Naumann
Keyword(s):  
Human Melanoma ◽  
Thin Sections ◽  
Ft Ir

scholarly journals One Pot Synthesis of Schiff Base Complexes of Cu(II), Zn(II), Ni(II), Co(II) and their Cytotoxicity, Molecular Docking and Antibacterial Activity

2020 ◽  
Vol 32 (10) ◽  
pp. 2507-2514
Author(s):  
A. Ashma ◽  
A. Sudha ◽  
S.L. Ashok Kumar ◽  
G. Sasikumar ◽  
S.J. Askar Ali
Keyword(s):  
Schiff Base ◽  
Cell Line ◽  
Atomic Ratio ◽  
Schiff Base Complexes ◽  
One Pot ◽  
Human Carcinoma ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
Cancer Activity

A novel Schiff base ligand (L), N(4)-cyclohexyl-2-(1-(5-chlorothiophen-2-yl)ethylidene)hydrazine carbothioamide was synthesized from isothiocyanatocyclohexane and hydrazine. All the synthesized transition metal(II) complexes were characterized by functional peak using 1H NMR, 13C NMR, FT-IR, UV-vis spectroscopy and metal complex atomic ratio of complexes of thiosemicarbazones were characterized by elemental analysis. Schiff base and its metal(II) complexes were screened in vitro medicinal drug activity against Gram positive and Gram-negative microorganisms and anti-cancer activity against human carcinoma cell line MCF-7 was detected with nickel(II) and cobalt(II) complexes. Further, the cytotoxity of ligand and its metal(II) complexes were tested on a Monkeys normal cell line and found to be non-toxic (< 200 μg/mL). In the present work, the molecular mechanism of anticancer (breast cancer) activity of the compounds were also disscussed.

Freeze-Etching of Intranuclear Adenovirus

1971 ◽  
Vol 29 ◽  
pp. 384-385
Author(s):  
Dennis T. Brown ◽  
Byron T. Burlingham
Keyword(s):  
Osmium Tetroxide ◽  
Adenovirus Type ◽  
Etching Time ◽  
Human Carcinoma ◽  
Thin Sections ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Infected Cells ◽  
Freeze Etching

Adenoviruses are revealed by negative staining to be icosahedral in structure and composed of 252 subunits (insert Fig. 1). The vertices have a fiber-like projection which has been implicated in the adsorbtion of the virus to the host cell. Ultra thin sections of adenovirus-infected cells has revealed that virus morphogenesis occurs in the nucleus.Human carcinoma (KB) tissue culture cells were productively infected with adenovirus type 2 at a multiplicity of 10 and incubated for 40 hours at 37°C. The infected monolayers were fixed in place with 1%, gluteraldehyde buffered in Millonigs phosphate buffer. The fixed cells were suspended in 30%, glycerol and processed according to the procedure of Moor in a Balzers 360 M freeze etch unit with an etching time of 70 seconds at -100°C. A second portion of the fixed cells in glycerol was post-fixed with 1% osmium tetroxide and embeded in epon 812 for ultrathin sectioning.

Characterization of Keratin Pearls in Oral Squamous Cell Carcinoma Using FT-IR Microspectroscopy

1997 ◽  
Vol 3 (S2) ◽  
pp. 833-834
Author(s):  
C.P. Schultz ◽  
K.-Z. Liu ◽  
P.D. Kerr ◽  
H.H. Mantsch
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Thin Sections ◽  
Thin Sectioning ◽  
Infrared Measurements ◽  
Ft Ir ◽  
Ir Microspectroscopy ◽  
Well Differentiated

Oral squamous cell carcinoma (SCC) positive biopsies from patients who underwent excision procedures by micrographical surgery were analyzed by FT-IR microspectroscopy in order to examine a specific fine structure of abnormal epithelial growth - the so-called keratin pearls. Taken from the oral cavity of 15 SCC positive patients, all biopsies indicated poorly or well-differentiated cancer containing smaller and larger pearls. In some cases only a few pearls were present; in others many of various sizes.After surgery the biopsies were rapidly frozen in liquid nitrogen and stored at -80°C before thin sectioning the tissue for infrared measurements. The frozen tissue was then sliced into 8 μm thin sections and dried on an infrared-transparent Bal2window. For comparison one slice of tissue was always used for hematoxylin and eosin staining by drying it on a standard microscope slide. This procedure creates a series of thin sections on BaF2 and glass following an alternate pattern of one 8 μm section for IR microspectroscopy and the next 8 μm one for microscopy.

FT-IR spectroscopic imaging of tissue thin sections

2001 ◽  
Author(s):  
Peter Lasch ◽  
Matthias Boese ◽  
Max Diem
Keyword(s):  
Spectroscopic Imaging ◽  
Thin Sections ◽  
Ft Ir ◽  
Ir Spectroscopic
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