Genetic Transformation of Hevea Brasiliensis (Rubber Tree) and Its Applications towards Crop Improvement and Production of Recombinant Proteins of Commercial Value

ABSTRACT Tension wood is an important anatomical structure for its participation in the orientation of the trunk and the architecture of the branches as a function of structural reinforcement. However, its presence in large amounts significantly affects the technological properties of wood, just as in the rubber tree. Nevertheless, there is still demand for information about the origin, distribution and structural features in this species. Thus, this study aims to characterize the cellular structures in tension and opposite wood in Hevea brasiliensis (rubber tree), as well as its radial and longitudinal distribution. Discs at the base and the middle of the commercial logs were collected from three trees in a commercial plantation located in Tabapoã - SP. Tangential diameter of vessels, fiber length (gelatinous and non-gelatinous fibers), microfibril angle and proportionality of cellular elements (vessels, axial parenchyma, ray, gelatinous fibers and non-gelatinous fibers) were measured, and influence of gelatinous fiber presence in vessel diameter was observed. Gelatinous fibers were observed in the two types of wood and in the two trunk heights. Both types of wood were distinguished by gelatinous fiber length and the proportion of axial parenchyma. The tension wood in mid-trunk was the most different, with long gelatinous fibers and less abundant, larger vessel diameter and vessel proportion. Moreover, smaller vessel diameter was observed in the regions with a high proportion of gelatinous fibers, suggesting that the plant invests more support than in liquid transport.

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Screening of endophytes from rubber trees (hevea brasiliensis) for biological control of Corticium salmonicolor

ENGINEERING AND TECHNOLOGY ◽

10.46223/hcmcoujs.tech.en.8.2.345.2018 ◽

2020 ◽

Vol 8 (2) ◽

pp. 38-44

Author(s):

Nguyen Van Minh ◽

Mai Huu Phuc ◽

Duong Nhat Linh ◽

Tran Thi A Ni ◽

Tran Kien Duc ◽

...

Keyword(s):

Biological Control ◽

Hevea Brasiliensis ◽

Endophytic Bacteria ◽

Rubber Tree ◽

Antagonistic Activity ◽

Ho Chi Minh City ◽

Viet Nam ◽

Dual Culture ◽

Living Tissue ◽

Tissue Samples

28 leaves and living-tissue samples of rubber tree (Hevea brasiliensis) were collected from Ho Chi Minh City, Binh Phuoc province and Binh Duong province (Viet Nam). We isolated and screened endophytes that have potential application as agents for biocontrol of Corticium salmonicolor, the agent of Pink Disease in rubber trees. As a result, 21 strains of endophytic bacteria and 14 strains of endophytic fungi were isolated. Antagonistic activity of the endophytes towards C. salmonicolor was checked by using a dual culture. Testing results showed that: T9, T15 and T16 strains have inhibited C. salmonicolor. T9 and T16 strains showed result that 100% of inhibiting C. salmonicolor at the concentration of 1:1. In the test of ability to kill C. salmonicolor, T9 and T16 strains showed that they could kill C. salmonicolor after 3 sprays of bacterial filtrate. T9 and T6 strains, which were identified by biochemical methods, have similar characteristics to Bacillus thuringiensis.

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Efficient Genetic Transformation of Rice for CRISPR/Cas9 Mediated Genome-Editing and Stable Overexpression Studies: A Case Study on Rice Lipase 1 and Galactinol Synthase Encoding Genes

Agronomy ◽

10.3390/agronomy12010179 ◽

2022 ◽

Vol 12 (1) ◽

pp. 179

Author(s):

Tanika Thakur ◽

Kshitija Sinha ◽

Tushpinder Kaur ◽

Ritu Kapoor ◽

Gulshan Kumar ◽

...

Keyword(s):

Genetic Transformation ◽

Crop Improvement ◽

Rice Cultivar ◽

Climatic Conditions ◽

Rice Transformation ◽

Transformation Protocol ◽

Galactinol Synthase ◽

Biotic Stresses ◽

Putative Transformants ◽

Efficient Genetic Transformation

Rice is a staple food crop for almost half of the world’s population, especially in the developing countries of Asia and Africa. It is widely grown in different climatic conditions, depending on the quality of the water, soil, and genetic makeup of the rice cultivar. Many (a)biotic stresses severely curtail rice growth and development, with an eventual reduction in crop yield. However, for molecular functional analysis, the availability of an efficient genetic transformation protocol is essential. To ensure food security and safety for the continuously increasing global population, the development of climate-resilient crops is crucial. Here, in this study, the rice transformation protocol has been effectively optimized for the efficient and rapid generation of rice transgenic plants. We also highlighted the critical steps and precautionary measures to be taken while performing the rice transformation. We further assess the efficacy of this protocol by transforming rice with two different transformation constructs for generating galactinol synthase (GolS) overexpression lines and CRISPR/Cas9-mediated edited lines of lipase (Lip) encoding the OsLip1 gene. The putative transformants were subjected to molecular analysis to confirm gene integration/editing, respectively. Collectively, the easy, efficient, and rapid rice transformation protocol used in this present study can be applied as a potential tool for gene(s) function studies in rice and eventually to the rice crop improvement.

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