Symbiotic performance of some modified Rhizobium etli strains in assays with Phaseolus vulgaris beans that have a high capacity to fix N2

1998 ◽  
pp. 89-94 ◽  
Author(s):  
E. Martínez-Romero ◽  
I. Hernández-Lucas ◽  
J. J. Peña-Cabriales ◽  
J. Z. Castellanos
Keyword(s):  
Phaseolus Vulgaris ◽  
High Capacity ◽  
Rhizobium Etli

Nodulation ofPhaseolus vulgarisbyRhizobium etliis enhanced by the presence ofBacillus

1997 ◽  
Vol 43 (1) ◽  
pp. 1-8 ◽  
Author(s):  
M. Srinivasan ◽  
F. B. Holl ◽  
D. J. Petersen
Keyword(s):  
Phaseolus Vulgaris ◽  
Lateral Root ◽  
Root Formation ◽  
Bacterial Species ◽  
Plant Roots ◽  
Rhizobium Etli ◽  
Phaseolus Acutifolius ◽  
Plant Interaction ◽  
Split Root ◽  
Bacillus Spp

The ability of Bacillus spp. to alter the nodulation of Phaseolus vulgaris by Rhizobium etli was assessed. The simultaneous presence of both Rhizobium etli TAL 182 and Bacillus megaterium S49 on plant roots during the early stages of plant growth was necessary for enhanced nodulation of Phaseolus vulgaris by the Rhizobium microsymbiont. Coinoculation with both bacterial species also facilitated heterologous nodulation of Rhizobium TAL 182 on Phaseolus acutifolius. These results are consistent with earlier reports of increased root hair proliferation and lateral root formation in response to coinoculation. Split-root experiments revealed that coinoculation partially suppressed host-controlled regulation of nodulation, implicating a plant interaction with the two bacterial species. Changes to the nodulation potential of R. etli due to coinoculation with Bacillus spp. demonstrate the potential for root-associated organisms other than rhizobia to alter the dynamics of the legume–Rhizobium symbiosis.Key words: Bacillus, nodulation enhancement, heterologous nodulation.

Characterization of Rhizobium etli and other Rhizobium spp. that nodulate Phaseolus vulgaris L. in an Austrian soil

1997 ◽  
Vol 6 (7) ◽  
pp. 601-608 ◽  
Author(s):  
A. Sessitsch ◽  
G. Hardarson ◽  
A. D. L. Akkermans ◽  
W. M. De Vos
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizobium Etli ◽  
Phaseolus Vulgaris L ◽  
Rhizobium Spp

Phaseolus vulgaris is nodulated in northern Spain by Rhizobium leguminosarum strains harboring two nodC alleles present in American Rhizobium etli strains: biogeographical and evolutionary implications

2010 ◽  
Vol 56 (8) ◽  
pp. 657-666 ◽  
Author(s):  
Paula García-Fraile ◽  
Daniel Mulas-García ◽  
Alvaro Peix ◽  
Raúl Rivas ◽  
Fernando González-Andrés ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizobium Leguminosarum ◽  
Intergenic Spacer ◽  
Climatic Conditions ◽  
Phylogenetic Group ◽  
Rhizobium Etli ◽  
Symbiotic Genes ◽  
Northern Spain ◽  
Major Producer ◽  
Core Genes

In this study a collection of rhizobial strains were isolated from effective nodules of Phaseolus vulgaris in a wide region of northern Spain, which is the major producer region of this legume in Spain. The analysis of their core genes, rrs, atpD, and recA, and the 16S–23S intergenic spacer showed that all isolates belong to the phylogenetic group of Rhizobium leguminosarum and some of them were identical to those of strains nodulating Vicia or Trifolium . None of the isolates was identified as Rhizobium etli ; however, all of them carry the nodC alleles α and γ harboured by American strains of this species. These alleles were also found in strains nodulating P. vulgaris in southern Spain identified as R. etli. These results suggest that R. etli was carried from America to Spain with common bean seeds, but that they could have found difficulties persisting in the soils of northern Spain, probably because of the climatic conditions. The symbiotic genes of this species could have been transferred, after the arrival of P. vulgaris, to strains of R. leguminosarum already present in northern Spanish soils.

Phosphoproteomic Analysis in Phaseolus vulgaris Roots Treated with Rhizobium etli Nodulation Factors

2016 ◽  
Vol 34 (5) ◽  
pp. 961-969 ◽  
Author(s):  
David Jáuregui-Zúñiga ◽  
Yolanda Ortega-Ortega ◽  
Martha Pedraza-Escalona ◽  
Juan Pablo Reyes-Grajeda ◽  
María Isabel Ruiz ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizobium Etli ◽  
Nodulation Factors

Analysis of the Proteome of Common Bean (Phaseolus vulgaris L.) Roots after Inoculation with Rhizobium etli

2012 ◽  
Vol 19 (8) ◽  
pp. 880-889 ◽  
Author(s):  
Afshin Salavati ◽  
Alireza Taleei ◽  
Ali Akbar Shahnejat Bushehri ◽  
Setsuko Komatsu
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rhizobium Etli ◽  
Phaseolus Vulgaris L

scholarly journals The Rhizobium etli argC Gene Is Essential for Arginine Biosynthesis and Nodulation of Phaseolus vulgaris

2001 ◽  
Vol 14 (2) ◽  
pp. 250-254 ◽  
Author(s):  
Simona Ferraioli ◽  
Rosarita Taté ◽  
Emilia Caputo ◽  
Alessandro Lamberti ◽  
Anna Riccio ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Mutant Strain ◽  
Wild Type Strain ◽  
Reductase Activity ◽  
Homologous Gene ◽  
Rhizobium Etli ◽  
Wild Type ◽  
Arginine Biosynthesis ◽  
Nodulation Factors ◽  
Wild Type Parent

A Tn5-induced mutant strain (CTNUX5) of Rhizobium etli unable to grow with ammonium as the sole nitrogen source was isolated and characterized. Sequence analysis showed that Tn5 is inserted into an argC-homologous gene. Unlike its wild-type parent (strain CE3), the mutant strain CTNUX5 had an absolute dependency on arginine to grow. The argC gene was cloned from the wild-type strain CE3, and the resulting plasmid, pAR207, after transformation was shown to relieve the arginine auxotrophy of strain CTNUX5. Unlike strain CE3 or CTNUX5-pAR207, strain CTNUX5 showed undetectable levels of N-acetyl-γ-glutamylphosphate reductase activity. Unless ar-ginine was added to the growth medium, strain CTNUX5 was unable to produce flavonoid-inducible lipo-chitin oli-gosaccharides (nodulation factors) and to induce nodules or nodulelike structures on the roots of Phaseolus vulgaris.

scholarly journals Inhibition of Rhizobium etli Polysaccharide Mutants by Phaseolus vulgaris Root Compounds

1994 ◽  
Vol 60 (9) ◽  
pp. 3315-3322 ◽  
Author(s):  
Linda Eisenschenk ◽  
Ronald Diebold ◽  
Jeanett Perez-Lesher ◽  
Andrew C. Peterson ◽  
N. Kent Peters ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Rhizobium Etli

scholarly journals luxI- and luxR-Homologous Genes of Rhizobium etli CNPAF512 Contribute to Synthesis of Autoinducer Molecules and Nodulation of Phaseolus vulgaris

1998 ◽  
Vol 180 (4) ◽  
pp. 815-821 ◽  
Author(s):  
Viola Rosemeyer ◽  
Jan Michiels ◽  
Christel Verreth ◽  
Jos Vanderleyden
Keyword(s):  
Phaseolus Vulgaris ◽  
Homoserine Lactone ◽  
Inhibitory Effect ◽  
Homologous Gene ◽  
Gram Negative Bacteria ◽  
Nitrogen Fixing ◽  
Rhizobium Etli ◽  
Homologous Genes ◽  
Growth Inhibitory ◽  
Enhanced Expression

ABSTRACT Autoinduction plays an important role in intercellular communication among symbiotic and pathogenic gram-negative bacteria. We report here that a nitrogen-fixing symbiont of Phaseolus vulgaris, Rhizobium etli CNPAF512, produces at least seven different autoinducer molecules. One of them exhibits a growth-inhibitory effect like that of the bacteriocin small[N-(3R-hydroxy-7-cis-tetradecanoyl)-l-homoserine lactone]. At least two of the other autoinducers are synthesized by a LuxI-homologous autoinducer synthase. The correspondingluxI homologous gene (raiI) and aluxR homolog (raiR) have been identified and characterized. Enhanced expression of raiI is dependent on cell density and on the presence of one or more autoinducer molecules synthesized by R. etli CNPAF512. A raiI mutant was shown to release only three different autoinducer molecules; araiR mutant releases four different autoinducer molecules. Examination of different mutants for nodulation of beans showed thatraiI is involved in the restriction of nodule number, whereas nitrogen-fixing activity in terms of acetylene reduction per nodule was not affected.

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