Attempts for Biological Control of Ralstonia solanacearum by Using Beneficial Microorganisms

Author(s):  
Pramod Kumar Sahu ◽  
Amrita Gupta ◽  
Kedarnath ◽  
Poonam Kumari ◽  
G. Lavanya ◽  
...  
2019 ◽  
Vol 2 (3) ◽  
pp. 89-96 ◽  
Author(s):  
Rachmad Saputra ◽  
Triwidodo Arwiyanto ◽  
Arif Wibowo

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.


2017 ◽  
Vol 12 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Silva Oliveira Wagner ◽  
Lima Coelho Iwanne ◽  
Rafaella Sousa Oliveira Jessica ◽  
Camila Barros Silva Leite Maria ◽  
Montenegro Stamford Arnaud Thatiana ◽  
...  

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Kamal A. M. Abo-Elyousr ◽  
Sabry A. Hassan

Abstract Background Bacterial wilt of tomato (BWP) caused by Ralstonia solanacearum (Smith) is a very important disease. Biological control of this disease is a very important tool to protect the plant and environment from pollution of chemical control. Results Twenty isolates of genus, Pantoea were isolated from healthy tomato root. Out of 20 isolates, 2 strains, PHYTPO1 and PHYTPO2, showed highly antagonistic property to control the growth of R. solanacearum in vitro conditions. They were identified as P. agglomerans by using 16S rRNA nucleotide sequence analysis. The 2 isolates were selected to study their effect (as cell suspension or culture filtrate) on the bacterial wilt under greenhouse conditions. PHYTPO1 inhibited maximum growth reduction of R. solanacearum and formed 2.5 cm2 of inhibition zone, followed by 1.2 cm2 in PHYTOPO2 under in vitro conditions. Treating with both isolates of P. agglomerans was significantly reduced disease severity of tomato wilt disease. The disease severity was reduced to 74.1 when treated as cell suspension, while when treated as culture filtrate, it reduced the disease severity up to 69.4 than infected control. Conclusion The strains of Pantoea can be used as an ecofriendly method to control of the most economic pathogen of tomato under greenhouse conditions. Further study is needed to find an appropriate formulation and approving application of these bacteria under field conditions.


1998 ◽  
pp. 337-342 ◽  
Author(s):  
J. J. Smith ◽  
L. C. Offord ◽  
G. N. Kibata ◽  
Z. K. Murimi ◽  
A. Trigalet ◽  
...  

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