Seed Health Testing and Seed Certification

Abstract: Cotton Ramulosis (Gossypium hirsutum) is an important disease affecting cotton plantations in Brazil, and its causal agent, Colletotrichum gossypiivar.cephalosporioides(Cgc), according to the Brazilian phytosanitary authority, was considered a regulated non quarantine pest. It makes this microorganism subject to standardization in seed certification programs. The current seed health testing for detecting that pathogen in seed samples does not provide reliable results for routine analysis. On this paper, attempts were made to design specific primers for detection of Cgc associated with cotton seed. Two primer sets were selected based on the analysis of a multiple alignment of gene’s sequence encoding the glyceraldehyde 3-phosphate dehydrogenase from Cgc, C. gossypii and reference strains of the C. gloeosporioides species complex. The conserved sites unique to Cgc strains were used to design specific fragment of 140 bp. The primer specificity was confirmed by using other fungi. The primers produced a detectable band of target DNA of Cgc in all inoculum potentials of the pathogen artificially inoculated by the water restriction technique. The developed primer pair represents, therefore, a reliable and rapid mean to diagnose the Ramulosis agent in cotton seed.

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The role of seed testing

NZGA: Research and Practice Series ◽

10.33584/rps.1.1979.3277 ◽

1980 ◽

Vol 1 ◽

pp. 103-109

Author(s):

D.J. Scott

Keyword(s):

New Zealand ◽

Key Words ◽

Seed Quality ◽

Seed Vigour ◽

Seed Certification ◽

Seed Testing ◽

Seed Health ◽

Quality Testing ◽

The Government

An account is given of the place of seed testing in New Zealand agriculture. The functions of the Government Seed Testing Station are outlined, including sections on the role of testing in the Government seed certification scheme, testing seed for export, seed importation. noxious plants, Rhizobium testing, surveys and research. Also discussed are the procedures and aspects of seed quality testing relevant to New Zealand pasture seeds, including sampling, purity, germination, seed health. seed vigour, cultivar and tolerances. Key words: Seed testing, New Zealand, noxious plants, Rhizobium.

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Size-Selective Sieving for Detecting Teliospores of Tilletia indica in Wheat Seed Samples

Plant Disease ◽

10.1094/pdis.2000.84.9.999 ◽

2000 ◽

Vol 84 (9) ◽

pp. 999-1007 ◽

Cited By ~ 20

Author(s):

G. L. Peterson ◽

M. R. Bonde ◽

J. G. Phillips

Keyword(s):

Tilletia Indica ◽

Seed Certification ◽

Teliospore Germination ◽

Detection And Identification ◽

Direct Microscopic Examination ◽

Seed Health ◽

Sample Extraction ◽

Polymerase Chain ◽

Phytosanitary Regulation ◽

Time Required

A method was developed to isolate teliospores of Tilletia indica from infested grain. The technique was evaluated to determine its sensitivity for detection and quantification of teliospores, the time required to conduct an individual test, and its utility for the detection and identification of the pathogen for phytosanitary regulation and seed certification. A seed wash of a 50-g grain sample was washed through 53-μm and 20-μm pore size nylon screens to remove unwanted debris and to concentrate and isolate teliospores. The material retained in the 20-μm screen was suspended for direct microscopic examination or plated on water agar for teliospore germination and identification by polymerase chain reaction (PCR) utilizing two pairs of T. indica-specific primers. The reliability of detection for both light microscopy and PCR are 100% at an infestation of five teliospores per 50-g sample. The proportion of teliospores recovered from grain samples artificially infested with T. indica was 0, 82, 88, 81, and 82%, respectively, at infestation levels of 0, 1, 2, 5, and 10 teliospores per 50-g wheat sample. Extraction efficiency was comparable to the centrifuge seed-wash method currently used by most seed health laboratories. Sample analysis using size-selective sieving was more than 83% faster than the standard centrifuge seed wash.

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Post harvest preservation in different containers, pathogenesis and management of chickpea (Cicer arietinumL.) seed health

Biodiversity International Journal ◽

10.15406/bij.2018.02.00113 ◽

2018 ◽

Vol 2 (6) ◽

pp. 562-565

Author(s):

Ashok Sadhu Kandhare

Keyword(s):

Post Harvest ◽

Seed Health

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Improved seed health tests for Xanthomonas axonopodis pv. phaseoli in common bean

10.31274/etd-180810-279 ◽

2010 ◽

Author(s):

Yiqing He

Keyword(s):

Common Bean ◽

Xanthomonas Axonopodis ◽

Seed Health

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Development of Material Combination Model Considering Economics and Construction Efficiency for G-SEED Certification

Sustainability ◽

10.3390/su13063535 ◽

2021 ◽

Vol 13 (6) ◽

pp. 3535

Author(s):

Byung-Ju Jeon ◽

Byung-Soo Kim

Keyword(s):

Structural Design ◽

Material Selection ◽

Selection Model ◽

Construction Costs ◽

Seed Certification ◽

Evaluation Scores ◽

The Cost ◽

Material Information ◽

Business As Usual ◽

Construction Efficiency

The Korean government proposed a goal to reduce its greenhouse gas emissions by 37% compared to business-as-usual levels by 2030 and launched the Green Standard for Energy and Environmental Design (G-SEED) certification system. The certification requires meeting the required score and material selection with a secured economy and construction efficiency. However, most buildings only focus on obtaining the certification scores instead of choosing economical materials with high construction efficiency. This research focused on developing a material selection model that considers both the construction efficiency and economy of the materials and the acquisition of material and resource evaluation scores from the G-SEED certification. This research, therefore, analyzed actual data to automate the material selection and compare alternatives to using a genetic algorithm to obtain optimized alternatives. This model proposes an alternative to constructability and economy when the required score and material information is entered. When the model was applied to actual cases, the result revealed a reduction in construction costs of about 37% compared to the cost with the traditional methods. The material selection model from this research can benefit construction project owners in terms of cost reduction, designers in terms of structural design time, and constructors in terms of construction efficiency

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A novel approach for Jatropha curcas seed health analysis based on multispectral and resonance imaging techniques

Industrial Crops and Products ◽

10.1016/j.indcrop.2020.113186 ◽

2021 ◽

Vol 161 ◽

pp. 113186

Author(s):

Clíssia Barboza da Silva ◽

Vitor de Jesus Martins Bianchini ◽

André Dantas de Medeiros ◽

Maria Heloisa Duarte de Moraes ◽

Agide Gimenez Marassi ◽

...

Keyword(s):

Jatropha Curcas ◽

Imaging Techniques ◽

Resonance Imaging ◽

Novel Approach ◽

Seed Health

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Development of Molecular Markers for Detection of Acidovorax citrulli Strains Causing Bacterial Fruit Blotch Disease in Melon

International Journal of Molecular Sciences ◽

10.3390/ijms20112715 ◽

2019 ◽

Vol 20 (11) ◽

pp. 2715 ◽

Cited By ~ 1

Author(s):

Md. Rafiqul Islam ◽

Mohammad Rashed Hossain ◽

Hoy-Taek Kim ◽

Denison Michael Immanuel Jesse ◽

Md. Abuyusuf ◽

...

Keyword(s):

Infected Leaf ◽

Bacterial Fruit Blotch ◽

Early Disease Detection ◽

Acidovorax Citrulli ◽

Seed Health ◽

Field Samples ◽

Whole Genomes ◽

Pathogenesis Related ◽

Primer Sets ◽

Inoculation Assay

Acidovorax citrulli (A. citrulli) strains cause bacterial fruit blotch (BFB) in cucurbit crops and affect melon significantly. Numerous strains of the bacterium have been isolated from melon hosts globally. Strains that are aggressively virulent towards melon and diagnostic markers for detecting such strains are yet to be identified. Using a cross-inoculation assay, we demonstrated that two Korean strains of A. citrulli, NIHHS15-280 and KACC18782, are highly virulent towards melon but avirulent/mildly virulent to the other cucurbit crops. The whole genomes of three A. citrulli strains isolated from melon and three from watermelon were aligned, allowing the design of three primer sets (AcM13, AcM380, and AcM797) that are specific to melon host strains, from three pathogenesis-related genes. These primers successfully detected the target strain NIHHS15-280 in polymerase chain reaction (PCR) assays from a very low concentration of bacterial gDNA. They were also effective in detecting the target strains from artificially infected leaf, fruit, and seed washing suspensions, without requiring the extraction of bacterial DNA. This is the first report of PCR-based markers that offer reliable, sensitive, and rapid detection of strains of A. citrulli causing BFB in melon. These markers may also be useful in early disease detection in the field samples, in seed health tests, and for international quarantine purposes.

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