Microfluidic and Non-microfluidic Methods of Sperm Sorting and Sperm Analysis

2021 ◽  
pp. 35-50
Author(s):  
Vinod Kumar Yata
Keyword(s):  
Sperm Analysis ◽  
Sperm Sorting

Сравнительная оценка спермопродукции чистопородных и гибридных хряков-производителей

2017 ◽  
pp. 2-6
Author(s):  
Байлар Садраддинович Иолчиев ◽  
Анна Валиевна Таджиева ◽  
Павел Михайлович Кленовицкий ◽  
Вугар Алиевич Багиров ◽  
Александр Алексеевич Никишов ◽  
...  
Keyword(s):  
Computer Assisted ◽  
Sperm Analysis ◽  
Computer Assisted Sperm Analysis

Практической частью репродуктивной технологии является прогноз и оценка фертильности самцов. Существующие методы оценки семени не дают достаточно корректного прогноза результатов осеменения и, следовательно, репродуктивного потенциала того или иного производителя. В работе приведены данные комплексной оценки биологической полноценности семени чистопородных и гибридных хряков-производителей с использованием компьютерной технологии CASA (computer-assisted sperm analysis). Оценивали активность сперматозоидов в образцах классическим способом (микроскопирование) и с помощью компьютерной технологии, программа «Зоосперм 1.0». Исследования проводили в 2014 – 2015 гг. в Лаборатории репродуктивной криобиологии животных (Россия). Объектом исследования служила сперма чистопородных и гибридных хряков-производителей, разводимых в разных регионах Российской Федерации: крупная белая (n = 24), дюрок (n = 28), ландрас (n = 18), пьетрен (n = 8), гибридные (n = 6). Установлено, что высокими репродуктивными качествами отличаются хряки-производители породы ландрас и гибридов, от них в среднем за эякулят получено 290,2 и 290,6 мл спермы соответственно; наименьшими — хряки-производители породы пьетрен (270 мл). Частота встречаемости сперматозоидов с аномальной морфологией высокая у хряков-производителей породы дюрок (12 %) и пьетрен (15 %). Частота встречаемости аномалии в разных сегментах сперматозоидов зависит от индивидуальной особенности хряков-производителей. У одних производителей наиболее часто встречаются морфологические отклонения головки, например, у хряка породы ландрас оно составило 58,60 %, у других часто встречались отклонения от нормы в строении жгутика (12,1 – 29,3 %). Использование компьютерной технологии при оценке биологической полноценности сперматозоидов хряков-производителей является наиболее точным и информативным.

scholarly journals Post-Thaw Sperm Quality and Functionality in the Autochthonous Pig Breed Gochu Asturcelta

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1885
Author(s):  
José Néstor Caamaño ◽  
Carolina Tamargo ◽  
Inmaculada Parrilla ◽  
Felipe Martínez-Pastor ◽  
Lorena Padilla ◽  
...  
Keyword(s):  
Genetic Resource ◽  
Sperm Quality ◽  
Genetic Material ◽  
Mitochondrial Activity ◽  
Computer Assisted ◽  
Kinematic Parameters ◽  
Linear Mixed Effects ◽  
Sperm Analysis ◽  
Computer Assisted Sperm Analysis ◽  
Analysis System

Genetic resource banks (GRB) preserve the genetic material of endangered, valuable individuals or genetically relevant breeds. Semen cryopreservation is a crucial technique to reach these goals. Thus, we aimed to assess the sperm parameters of semen doses from the native pig breed Gochu Asturcelta stored at the GRB of Principado de Asturias (GRB-PA, Gijón, Spain), focusing on intrinsic and extrinsic (boar, season) factors. Two straws per boar (n = 18, 8–71 months of age) were thawed, pooled, and assessed after 30 and 150 min at 37 °C by CASA (computer-assisted sperm analysis system; motility and kinematic parameters) and flow cytometry (viability, acrosomal status, mitochondrial activity, apoptosis, reactive oxygen species, and chromatin status). The effects of age, incubation, and season on post-thawing quality were determined using linear mixed-effects models. Parameters were on the range for commercial boar breeds, with chromatin status (SCSA: fragmentation and immaturity) being excellent. Incubation decreased sperm quality and functionality. The boar age did not have a significant effect (p > 0.05), but the between-boar variability was significant (p < 0.001). The season significantly affected many parameters (motility, kinematics, viability, acrosomal status, mitochondrial activity), especially after 150 min of incubation. In general, samples collected in spring and summer showed higher quality post-thawing, the lowest in winter. In conclusion, the sperm doses from the Gochu Asturcelta breed stored at the GRB-PA showed excellent chromatin status and acceptable characteristics after thawing. Therefore, boar and seasonal variability in this autochthonous breed could be relevant for cryobank management.

scholarly journals Author Correction: Advances in sperm analysis: techniques, discoveries and applications

2021 ◽  
Author(s):  
Changsheng Dai ◽  
Zhuoran Zhang ◽  
Guanqiao Shan ◽  
Lap-Tak Chu ◽  
Zongjie Huang ◽  
...  
Keyword(s):  
Sperm Analysis ◽  
Analysis Techniques

scholarly journals Thawing boar semen in the presence of seminal plasma improves motility, modifies subpopulation patterns and reduces chromatin alterations

2017 ◽  
Vol 29 (8) ◽  
pp. 1576 ◽  
Author(s):  
Rocío Fernández-Gago ◽  
Manuel Álvarez-Rodríguez ◽  
Marta E. Alonso ◽  
J. Ramiro González ◽  
Beatriz Alegre ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Chromatin Compaction ◽  
Sperm Analysis ◽  
Positive Effects ◽  
Fragmentation Index ◽  
Non Linear ◽  
Boar Semen ◽  
Dna Fragmentation Index

Seminal plasma could have positive effects on boar semen after thawing. In the present study we investigated changes in the motility and chromatin structure in spermatozoa over 4 h incubation (37°C) of boar semen thawed in the presence of 0%, 10% or 50% seminal plasma from good-fertility boars. Cryopreserved doses were used from seven males, three of which were identified as susceptible to post-thawing chromatin alterations. Motility was analysed by computer-aided sperm analysis every hour, and data were used in a two-step clustering, yielding three subpopulations of spermatozoa (slow non-linear, fast non-linear, fast linear). Chromatin structure was analysed using a sperm chromatin structure assay and flow cytometry to determine the DNA fragmentation index (%DFI) as a percentage, the standard deviation of the DFI (SD-DFI) and the percentage of high DNA stainability (%HDS), indicating chromatin compaction. Thawing without seminal plasma resulted in a rapid loss of motility, whereas seminal plasma helped maintain motility throughout the incubation period and preserved the subpopulation comprising fast and linear spermatozoa. The incidence of chromatin alterations was very low in samples from non-susceptible males, whereas samples from males susceptible to post-thawing chromatin alterations exhibited marked alterations in %DFI and %HDS. Seminal plasma partly prevented these alterations in samples from susceptible males. Overall, 50% seminal plasma was the most efficient concentration to protect motility and chromatin. Some changes were concomitant with physiological events reported previously (e.g., semen thawed with 50% seminal plasma increased the production of reactive oxygen species and yielded higher fertility after AI). Thawing in the presence of seminal plasma could be particularly useful in the case of samples susceptible to post-thawing chromatin damage.

A Substance Isolated from Cornus officinalis Enhances the Motility of Human Sperm

1997 ◽  
Vol 25 (03n04) ◽  
pp. 301-306 ◽  
Author(s):  
Hellen Jeng ◽  
Chao Mei Wu ◽  
Shuen-Jiing Su ◽  
Wen-Chang Chang
Keyword(s):  
Sperm Motility ◽  
Crude Extract ◽  
High Performance ◽  
Chinese Herb ◽  
Human Sperm ◽  
Final Concentration ◽  
Dried Fruits ◽  
Sperm Analysis ◽  
Cornus Officinalis ◽  
Phosphate Buffered Saline

The effects of a Chinese herb, Cornus officinalis, on the motility of human sperm was studied. An aqueous extract was prepared from the dried fruits of the herb and used in this study. The crude extract at a final concentration of 0.5 μg/μl in phosphate buffered saline (pH 7.4) increased sperm motility from 25.8 ± 7.7% to 42.8 ± 10.3% (i.e. 68% increase, n = 7), as determined by the computer-aided-sperm-analysis (CASA) method. The crude extract was fractionated by high-performance liquid chromatography (HPLC) into four fractions: Cl , C2, C3 and C4. Their effects on sperm motility were further studied by CASA. Only the C4 fraction showed substantial stimulatory effects on sperm motility. At a concentration of 5 ng/μl, C4 increased the sperm motility from 15.7 ± 3.8% to 34.5 ± 6.4% (i.e. 120% increase, n = 6) by CASA and from 14.9 ± 4.3 to 28.5 ± 8.1 (i.e. 91% increase, n = 8) by transmembrane migration ratio (TMMR) method. This result suggests that C4 is the active component in Cornus officinalis that enhances sperm motility.

Automatic annular laser trapping: a system for high-throughput sperm analysis and sorting

2009 ◽  
Vol 2 (3) ◽  
pp. 167-177 ◽  
Author(s):  
Linda Shi ◽  
Bing Shao ◽  
Timothy Chen ◽  
Michael Berns
Keyword(s):  
High Throughput ◽  
Laser Trapping ◽  
Sperm Analysis ◽  
Annular Laser
Sign in / Sign up

Export Citation Format

Share Document