RFLP mapping of a gene in barley conferring resistance to net blotch (Pyrenophora teres)

Euphytica ◽  
1996 ◽  
Vol 91 (2) ◽  
pp. 229-234
Author(s):  
Andreas Graner ◽  
Bärbel Foroughi-Wehr ◽  
Andy Tekauz
Keyword(s):  
Rflp Mapping ◽  
Pyrenophora Teres ◽  
Net Blotch

Breeding barley for durable resistance to net and spot forms of net blotch

2021 ◽  
pp. 567-586
Author(s):  
Jerome D. Franckowiak ◽  
◽  
Gregory J. Platz ◽  
Keyword(s):  
Population Dynamics ◽  
Molecular Markers ◽  
Durable Resistance ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Net Form Net Blotch

This chapter focuses on breeding barley for durable resistance to net and spot forms of net blotch. It starts by reviewing how Pyrenophora teres f. teres can cause net form net blotch. The chapter then goes on to examine the molecular markers that can be identified to provide resistances to net form net blotch. A section on the population dynamics of barley–P. teres f. teres interactions is also provided. The chapter also reviews how breeding crops with specific genes can help to create durable resistance to net form blotch. It moves on to discuss how Pyrenophora teres Drechs. f. maculata can cause spot form net blotch and how identifying specific molecular markers can help provide resistance to this form of net blotch. The chapter concludes by highlighting the importance of combining durable resistance to both forms of net blotch.

Mapping of barley susceptibility/resistance QTL against spot form net blotch caused by Pyrenophora teres f. maculata using RIL populations

2019 ◽  
Vol 132 (7) ◽  
pp. 1953-1963 ◽  
Author(s):  
Prabin Tamang ◽  
Jonathan K. Richards ◽  
Abdullah Alhashal ◽  
Roshan Sharma Poudel ◽  
Richard D. Horsley ◽  
...  
Keyword(s):  
Pyrenophora Teres ◽  
Net Blotch ◽  
Resistance Qtl

Genetic analysis of a worldwide barley collection for resistance to net form of net blotch disease (Pyrenophora teres f. teres)

2019 ◽  
Vol 132 (9) ◽  
pp. 2633-2650 ◽  
Author(s):  
Fluturë Novakazi ◽  
Olga Afanasenko ◽  
Anna Anisimova ◽  
Gregory J. Platz ◽  
Rod Snowdon ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Pyrenophora Teres ◽  
Net Blotch

SOURCES OF RESISTANCE IN BARLEY TO PYRENOPHORA TERES

1965 ◽  
Vol 45 (2) ◽  
pp. 189-193 ◽  
Author(s):  
K. W. Buchannon ◽  
W. C. McDonald
Keyword(s):  
North Dakota ◽  
Quality Characteristics ◽  
Seedling Stage ◽  
Malting Quality ◽  
Western Canada ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Sources Of Resistance ◽  
Highly Pathogenic ◽  
Resistant Varieties

The reaction to infection by Pyrenophora teres Drechs., the incitant of net blotch of barley, was determined for 6,174 varieties in the U.S.D.A. World Barley Collection. Forty varieties, seventeen of them from Ethiopia, were resistant in the seedling stage to a highly pathogenic strain of the fungus prevalent in Western Canada and to composites of isolates from Manitoba, Saskatchewan, Alberta, Ontario, North Dakota, California, and Mexico. They were also resistant in the field at three locations in Western Canada. Agronomic and malting quality characteristics for the resistant varieties were also recorded.

Epidemiology and control of spot form of net blotch (Pyrenophora teres f. maculata) of barley: a review

2009 ◽  
Vol 60 (4) ◽  
pp. 303 ◽  
Author(s):  
Mark S. McLean ◽  
Barbara J. Howlett ◽  
Grant J. Hollaway
Keyword(s):  
Cost Effective ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Stubble Retention ◽  
Resistant Varieties ◽  
Cost Effective Method ◽  
Barley Germplasm ◽  
Grain Yield And Quality ◽  
The 1960S ◽  
And Control

Spot form of net blotch (SFNB), caused by the fungus Pyrenophora teres f. maculata, was first described in Denmark in the 1960s and is now a prevalent foliar disease of barley in many countries. This disease should be controlled as a separate disease-causing organism from the net form of net blotch (NFNB), which is caused by P. teres f. teres. The increase in prevalence of SFNB is primarily due to stubble retention and cultivation of susceptible varieties, which have resulted in increased inoculum. Infected barley stubble is the primary inoculum source for SFNB, producing both asexual spores (conidia) and sexual spores (ascospores) from pseudothecia. Spot form of net blotch causes significant losses in grain yield and quality in situations where inoculum is present, susceptible varieties are cultivated, and where the climate is cool and moist. Cultivation of resistant varieties is the most cost-effective method for control of SFNB and more than 12 different resistance sources have been identified in barley germplasm and wild barley relatives. The resistance loci of 11 of these have been mapped. Control of SFNB can also be achieved with application of foliar fungicides, crop rotation, and stubble destruction.

scholarly journals A nested association mapping population identifies multiple small effect QTL conferring resistance against net blotch (Pyrenophora teres f. teres) in wild barley

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0186803 ◽  
Author(s):  
Thomas Vatter ◽  
Andreas Maurer ◽  
Doris Kopahnke ◽  
Dragan Perovic ◽  
Frank Ordon ◽  
...  
Keyword(s):  
Association Mapping ◽  
Wild Barley ◽  
Mapping Population ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Nested Association Mapping ◽  
Nested Association Mapping Population ◽  
Association Mapping Population

Impact of seedbed utilization and fungicide application on severity of net blotch [Pyrenophora teres] and production of barley

2004 ◽  
Vol 26 (4) ◽  
pp. 533-547 ◽  
Author(s):  
T.K. Turkington ◽  
G.W. Clayton ◽  
K.N. Harker ◽  
H.R. Kutcher ◽  
J.T. O'Donovan ◽  
...  
Keyword(s):  
Pyrenophora Teres ◽  
Net Blotch ◽  
Fungicide Application

scholarly journals Rare Pyrenophora teres Hybridization Events Revealed by Development of Sequence-Specific PCR Markers

2017 ◽  
Vol 107 (7) ◽  
pp. 878-884 ◽  
Author(s):  
Barsha Poudel ◽  
Simon R. Ellwood ◽  
Alison C. Testa ◽  
Mark McLean ◽  
Mark W. Sutherland ◽  
...  
Keyword(s):  
Host Plants ◽  
Fragment Length Polymorphism ◽  
Putative Hybrid ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Pcr Markers ◽  
Chain Reaction ◽  
Specific Pcr ◽  
Polymerase Chain ◽  
Pathogenic Variability

Pyrenophora teres f. teres and P. teres f. maculata cause net form and spot form, respectively, of net blotch on barley (Hordeum vulgare). The two forms reproduce sexually, producing hybrids with genetic and pathogenic variability. Phenotypic identification of hybrids is challenging because lesions induced by hybrids on host plants resemble lesions induced by either P. teres f. teres or P. teres f. maculata. In this study, 12 sequence-specific polymerase chain reaction markers were developed based on expressed regions spread across the genome. The primers were validated using 210 P. teres isolates, 2 putative field hybrids (WAC10721 and SNB172), 50 laboratory-produced hybrids, and 7 isolates collected from barley grass (H. leporinum). The sequence-specific markers confirmed isolate WAC10721 as a hybrid. Only four P. teres f. teres markers amplified on DNA of barley grass isolates. Amplified fragment length polymorphism markers suggested that P. teres barley grass isolates are genetically different from P. teres barley isolates and that the second putative hybrid (SNB172) is a barley grass isolate. We developed a suite of markers which clearly distinguish the two forms of P. teres and enable unambiguous identification of hybrids.

Proteomic analysis reveals the potential involvement of xylanase from Pyrenophora teres f. teres in net form net blotch disease of barley

2014 ◽  
Vol 43 (6) ◽  
pp. 715-726 ◽  
Author(s):  
I. A. Ismail ◽  
D. Godfrey ◽  
A. J. Able
Keyword(s):  
Proteomic Analysis ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Net Form Net Blotch

scholarly journals Mating Type Locus-Specific Polymerase Chain Reaction Markers for Differentiation of Pyrenophora teres f. teres and P. teres f. maculata, the Causal Agents of Barley Net Blotch

2010 ◽  
Vol 100 (12) ◽  
pp. 1298-1306 ◽  
Author(s):  
Shunwen Lu ◽  
Gregory J. Platz ◽  
Michael C. Edwards ◽  
Timothy L. Friesen
Keyword(s):  
Polymerase Chain Reaction ◽  
Mating Type ◽  
Pathogen Detection ◽  
Epidemiological Studies ◽  
Pyrenophora Teres ◽  
Nucleotide Polymorphisms ◽  
Net Blotch ◽  
Chain Reaction ◽  
Type Locus ◽  
Polymerase Chain

Fourteen single nucleotide polymorphisms (SNPs) were identified at the mating type (MAT) loci of Pyrenophora teres f. teres (Ptt), which causes net form (NF) net blotch, and P. teres f. maculata (Ptm), which causes spot form (SF) net blotch of barley. MAT-specific SNP primers were developed for polymerase chain reaction (PCR) and the two forms were differentiated by distinct PCR products: PttMAT1-1 (1,143 bp) and PttMAT1-2 (1,421 bp) for NF MAT1-1 and MAT1-2 isolates; PtmMAT1-1 (194 bp) and PtmMAT1-2 (939 bp) for SF MAT1-1 and MAT1-2 isolates, respectively. Specificity was validated using 37 NF and 17 SF isolates collected from different geographic regions. Both MAT1-1 and MAT1-2 SNP primers retained respective specificity when used in duplex PCR. No cross-reactions were observed with DNA from P. graminea, P. tritici-repentis, or other ascomycetes, or barley. Single or mixed infections of the two different forms were also differentiated. This study provides the first evidence that the limited SNPs at the MAT locus are sufficient for distinguishing closely related heterothallic ascomycetes at subspecies levels, thus allowing pathogenicity and mating type characteristics of the fungus to be determined simultaneously. Methods presented will facilitate pathogen detection, disease management, and epidemiological studies.

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