The effect of alternating temperatures on the self-incompatibility of some clones of Brussels sprouts (Brassica oleracea L. var. Gemmifera (DC.) Schulz)

Euphytica ◽  
1977 ◽  
Vol 26 (2) ◽  
pp. 273-277 ◽  
Author(s):  
D. L. Visser
Keyword(s):  
Brassica Oleracea ◽  
The Self ◽  
Self Incompatibility ◽  
Brussels Sprouts ◽  
Brassica Oleracea L

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

Variability of the self-incompatibility reaction in Brassica oleracea L. with S 15 haplotype

2009 ◽  
Vol 23 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Houria Hadj-Arab ◽  
Anne-Marie Chèvre ◽  
Thierry Gaude ◽  
Véronique Chable
Keyword(s):  
Brassica Oleracea ◽  
The Self ◽  
Self Incompatibility ◽  
Incompatibility Reaction ◽  
Brassica Oleracea L

The effect of aerated hydration on DNA synthesis in embryos of Brassica oleracea L.

1993 ◽  
Vol 3 (3) ◽  
pp. 195-199 ◽  
Author(s):  
J. M. Thornton ◽  
A. R. S. Collins ◽  
A. A. Powell
Keyword(s):  
Dna Damage ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Brassica Oleracea ◽  
Thymidine Incorporation ◽  
Recovery Process ◽  
Time Difference ◽  
Brussels Sprouts ◽  
Brassica Oleracea L

AbstractAgeing causes a delay in the onset of DNA replication in seeds. Aerated hydration for 8 h, a treatment to invigorate seeds, resulted in a reduction of about 12 h in the time difference in the onset of DNA synthesis between unaged and aged embryos of brassica seed. This effect is consistent with a recovery process occurring during aerated hydration of the seed involving the repair of accumulated DNA damage. The occurrence of hydroxyurea-resistant 3H-thymidine incorporation in aged Brussels sprouts embryos during the period 16–32 h from the start of hydration supports this interpretation.

Test of Interaction Between SRK and THL1 from Brassica oleracea L. in Self-Incompatibility Signaling Process

2008 ◽  
Vol 34 (6) ◽  
pp. 934-943
Author(s):  
Qi-Guo GAO ◽  
Ming SONG ◽  
Yi NIU ◽  
Kun YANG ◽  
Li-Quan ZHU ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Self Incompatibility ◽  
Signaling Process ◽  
Brassica Oleracea L

scholarly journals Immunogenetics of self-incompatibility in Brassica oleracea L.

Heredity ◽  
1967 ◽  
Vol 22 (4) ◽  
pp. 519-527 ◽  
Author(s):  
M E Nasrallah ◽  
D H Wallace
Keyword(s):  
Brassica Oleracea ◽  
Self Incompatibility ◽  
Brassica Oleracea L
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