Assessment of liquid culture procedures for in vitro propagation of Rheum emodi

1993 ◽  
Vol 34 (2) ◽  
pp. 223-226 ◽  
Author(s):  
Nand Lal ◽  
Paramvir Singh Ahuja
1970 ◽  
Vol 20 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Meenakshi Banerjee ◽  
Priyanka Modi

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots.  Rooted plantlets were successfully transferred to the field, after acclimation in the net house.   Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation   D.O.I. 10.3329/ptcb.v20i2.6917   Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)


1983 ◽  
Vol 63 (1) ◽  
pp. 311-316 ◽  
Author(s):  
ROBERT E. HARRIS ◽  
EDWIN B. B. MASON

Two machines are described, one to tilt 400 50-mL or 320 125-mL Erlenmeyer flasks, and the other to rock 120 455-mL or 70 910-mL square wide-mouth Mason jars, or combinations of the different container sizes. In grapes, explants transferred to liquid media on the machines after 28 days on agar produced seven times as many shoots in 90 days as explants maintained on agar media. Similar increases were obtained with Arctostaphylos uva ursi, Fuchsia hybrida ’Swingtime,’ Amelanchier alnifolia, and Nicotiana tabacum ’Xanthi-nc.’ The liquid culture method also reduces cost by using less media and agar.Key words: Machines, in vitro, propagation, liquid-media


Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
M Gangopadhyay ◽  
R Bhattacharya ◽  
D Chakraborty ◽  
S Bhattacharya ◽  
A Mitra ◽  
...  

Green Farming ◽  
2020 ◽  
Vol 11 (2-3) ◽  
pp. 130
Author(s):  
VIJAYAKUMARI N. ◽  
P. GHOSH ◽  
Y.B. LAHANE ◽  
K.P. FISKE

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