TWO MACHINES FOR IN VITRO PROPAGATION OF PLANTS IN LIQUID MEDIA

1983 ◽  
Vol 63 (1) ◽  
pp. 311-316 ◽  
Author(s):  
ROBERT E. HARRIS ◽  
EDWIN B. B. MASON
Keyword(s):  
Nicotiana Tabacum ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Method ◽  
The Other ◽  
Liquid Media ◽  
Wide Mouth ◽  
Agar Media ◽  
Two Machines

Two machines are described, one to tilt 400 50-mL or 320 125-mL Erlenmeyer flasks, and the other to rock 120 455-mL or 70 910-mL square wide-mouth Mason jars, or combinations of the different container sizes. In grapes, explants transferred to liquid media on the machines after 28 days on agar produced seven times as many shoots in 90 days as explants maintained on agar media. Similar increases were obtained with Arctostaphylos uva ursi, Fuchsia hybrida ’Swingtime,’ Amelanchier alnifolia, and Nicotiana tabacum ’Xanthi-nc.’ The liquid culture method also reduces cost by using less media and agar.Key words: Machines, in vitro, propagation, liquid-media

scholarly journals Micropropagation of Bacopa monnieri using Cyanobacterial Liquid Medium

1970 ◽  
Vol 20 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Meenakshi Banerjee ◽  
Priyanka Modi
Keyword(s):  
Plant Tissue ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Axillary Node ◽  
Bacopa Monnieri ◽  
Shoot Initiation ◽  
Aulosira Fertilissima

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots.  Rooted plantlets were successfully transferred to the field, after acclimation in the net house.   Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation   D.O.I. 10.3329/ptcb.v20i2.6917   Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)

scholarly journals A Versatile Liquid Culture Method to Control the <i>in Vitro</i> Development of Shoot and Root Apical Meristems of Bamboo Plants

2020 ◽  
Vol 11 (02) ◽  
pp. 262-275
Author(s):  
Most Tanziman Ara ◽  
Taiji Nomura ◽  
Yasuo Kato ◽  
Shinjiro Ogita
Keyword(s):  
Liquid Culture ◽  
Culture Method ◽  
Apical Meristems

scholarly journals Evaluation of the effects of agricultural LED lighting on in vitro propagation of Cordyceps militaris (Link.) Fries

2020 ◽  
Vol 17 (3) ◽  
pp. 473-481
Author(s):  
Do Hong Gam ◽  
Duong Huong Huynh ◽  
Phan Thi Lan Anh ◽  
Nguyen Hoang Duong ◽  
Do Thi Kim Hoa
Keyword(s):  
Growth And Development ◽  
In Vitro Propagation ◽  
Positive Impact ◽  
The Other ◽  
Fluorescent Light ◽  
Led Lighting ◽  
Fresh Biomass ◽  
Red Led ◽  
Led Lights

In this study, the effects of various agricultural LED lights (LED NN), including single red LED (R), single blue LED (B), and four combinations of blue, red, and warm white (W) LED (BR, BRW1, BRW2, BRW3) on the growth and development of C. militaris (Link.) Fries were evaluated in vitro. After 7 days, samples subjected to LED NN showed shorter sporocarp sprouting time and higher sprouting ratio than the control, which was subjected to T5 fluorescent light. After 2 months, LED lights with high red ratio, such as single red LED and LED BR, had suppressing effect on the growth and development of C. militaris (Link.) Fries. On the other hand, combinations of red, blue, and warm white such as LED BRW1, LED BRW2, and LED BRW3 had the positive impact on the growth and development of this fungus. Notably, samples subjected to LED BRW2 reached 5.79 cm in height, fresh biomass of 3.67 g/20 samples. Cordycepin and Adenosine levels were 64.2 and 6.37 mg/100 g fresh mass, respectively. All of studied  indicators were the higher compared to those of the control and other LED lighting schemes. Therefore, it can be conlcuded that LED lighting combination with BRW2 ratio of 1:5:1 and luminous intensity of 45±2 µmol.m-2.s-1 (511,59 Lux) was suitable for the growth and development of C. militaris (Link.) Friesand a potential replacement of fluorescent light for C. militaris (Link.) Friesin vitro propagation.

scholarly journals Static Liquid Culture of Doritaenopsis Seedlings

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 206-210 ◽  
Author(s):  
Wei-Ting Tsai ◽  
Chien-Young Chu
Keyword(s):  
Liquid Medium ◽  
Liquid Culture ◽  
Petri Dish ◽  
Liquid Media ◽  
Solid Culture ◽  
Sealing Material ◽  
Phase Liquid ◽  
Sealing Materials ◽  
Growth Of Seedlings

Methods for static liquid culture are described to improve the growth of Doritaenopsis (commercially known as Phalaenopsis) seedlings in vitro. The results showed that seeds not only germinated, but also grew faster in liquid medium. No hyperhydric seedlings were observed in liquid culture when liquid level was accurately controlled by culture density, medium volume, and sealing materials. Although the germination percent was unaffected by medium phase (liquid or solid), sowing density, medium volume, or sealing material, the growth of seedlings decreased as density increased or medium volume decreased. Seeds of 1.5 mg mixed with 20 mL of liquid medium per 9-cm petri dish sealed with two layers of parafilm prompted optimal results. Shoot growth also was enhanced while 75-day-old seedlings were subcultured in liquid media with or without support. Seedling growth was enhanced by adding 20 mL liquid media to 36 seedlings without support after 45 days of culture. It was expected that by static liquid culture, the period from sowing to ex vitro would be 1.5 months shorter than the traditional solid culture.

scholarly journals Enhancement of Campylobacter hepaticus culturing to facilitate downstream applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Canh Phung ◽  
Timothy B. Wilson ◽  
José A. Quinteros ◽  
Peter C. Scott ◽  
Robert J. Moore ◽  
...  
Keyword(s):  
Liquid Culture ◽  
Vaccine Development ◽  
Genomic Analysis ◽  
Bacterial Biomass ◽  
In Silico Analysis ◽  
Culture Method ◽  
Efficient Production ◽  
Culture Density ◽  
Brucella Broth

AbstractCampylobacter hepaticus causes Spotty Liver Disease (SLD) in chickens. C. hepaticus is fastidious and slow-growing, presenting difficulties when growing this bacterium for the preparation of bacterin vaccines and experimental disease challenge trials. This study applied genomic analysis and in vitro experiments to develop an enhanced C. hepaticus liquid culture method. In silico analysis of the anabolic pathways encoded by C. hepaticus revealed that the bacterium is unable to biosynthesise l-cysteine, l-lysine and l-arginine. It was found that l-cysteine added to Brucella broth, significantly enhanced the growth of C. hepaticus, but l-lysine or l-arginine addition did not enhance growth. Brucella broth supplemented with l-cysteine (0.4 mM), l-glutamine (4 mM), and sodium pyruvate (10 mM) gave high-density growth of C. hepaticus and resulted in an almost tenfold increase in culture density compared to the growth in Brucella broth alone (log10 = 9.3 vs 8.4 CFU/mL). The type of culture flask used also significantly affected C. hepaticus culture density. An SLD challenge trial demonstrated that C. hepaticus grown in the enhanced culture conditions retained full virulence. The enhanced liquid culture method developed in this study enables the efficient production of bacterial biomass and therefore facilitates further studies of SLD biology and vaccine development.

Perbandingan Metode Sterilisasi Untuk Perbanyakan Rubus rosifolius Secara in Vitro

2021 ◽  
Vol 14 (1) ◽  
pp. 127-137
Author(s):  
Muhammad Imam Surya ◽  
Lily Ismaini
Keyword(s):  
Tissue Culture ◽  
Ascorbic Acid ◽  
In Vitro Propagation ◽  
Povidone Iodine ◽  
Tween 80 ◽  
Botanical Garden ◽  
The Other ◽  
Fruit Crops ◽  
Plant Propagation

AbstrakRubus rosifolius adalah salah satu jenis rasberi liar yang memiliki potensi cukup tinggi untuk dikembangkan sebagai tanaman buah. Selain itu, metode perbanyakan tanaman merupakan salah satu faktor yang berpengaruh dalam pembudidayaan. Lebih lanjut, informasi terkait upaya perbanyakan R. rosifolius secara in vitro masih sangat terbatas. Percobaan ini ditujukan untuk mengetahui metode sterilisasi yang tepat pada eksplan R. rosifolius. Sebanyak 17 metode sterilisasi telah diujicobakan di Laboratorium Kultur Jaringan BKT Kebun Raya Cibodas-LIPI. Bahan sterilisasi yang digunakan, yaitu detergen, tween 80, bakterisida, fungisida, clorox/pemutih (NaClO), alkohol 70%, larutan Plant Preservative Mixture (PPM), vitamin C/asam askorbat, dan povidone iodine/antiseptik. Hasil percobaan menunjukkan bahwa metode sembilan merupakan metode sterilisasi yang cukup optimum untuk sterilisasi eksplan R. rosifolius. Metode sembilan mampu menghambat munculnya mikroorganisme endofitik hingga 8 hari dan tidak menyebabkan warna eksplan menjadi cokelat/browning. Tahapan sterilisasi pada metode sembilan meliputi pencucian dengan detergen, perendaman dengan bakterisida + fungisida selama +30 menit, perendaman dengan clorox 10% + tween 80 selama +15 menit, pencucian dengan larutan PPM selama +15 menit.  AbstractRubus rosifolius is one of the species from wild raspberries, which is has high potential to develop as a fruit crops. In the other hand, the technique of plant propagation became an important factor for cultivation. Moreover, the information related to the in vitro propagation of R. rosifolius is very limited. This experiment was aimed to determine the best method to sterilize an explants of R. rosifolius. About 17 methods of sterilization have been tried in the laboratorium of tissue culture at Cibodas Botanical Garden-Indonesian Institute of Sciences. The combination of detergent, tween 80, bactericide, fungicide, sodium hypochlorite (NaClO), alcohol 70%, plant preservative mixture (PPM), ascorbic acid, and povidone iodine were used during the experiment. The results show that the method of sterilization number nine could be inhibit the emergence of endophytic organisms for eight days and keep an explant in green with a little brownish compared by the others methods. The method of sterilization number nine was consist of several steps i.e. wash by detergent, soak in bactericide + fungicide for +30 minutes, soak in sodium hypoclorite 10% + tween 80 for +15 minutes, wash by PPM solution for +15 minutes.

Role of Sporormiellasimilis as a potential bioprotectant of Populustremuloides wood against the blue-stain fungus Ophiostomapiliferum

1994 ◽  
Vol 24 (11) ◽  
pp. 2235-2239 ◽  
Author(s):  
P. Chakravarty ◽  
L. Trifonov ◽  
L.J. Hutchison ◽  
Y Hiratsuka ◽  
W.A. Ayer
Keyword(s):  
Culture Filtrate ◽  
Liquid Culture ◽  
Biological Control Agent ◽  
Control Agent ◽  
Dual Culture ◽  
Blue Stain ◽  
Agar Media ◽  
Fungitoxic Effect

Interactions between Sporormiellasimilis Khan & Cain and the blue-stain fungus Ophiostomapiliferum (Fr.) H. & P. Sydow, both isolated from Populustremuloides Michx. wood, were investigated. Sporormiellasimilis significantly reduced the growth of O. piliferum in vitro when grown in dual culture, in addition to inhibiting the growth of O. piliferum on agar media and in liquid culture when treated with a culture filtrate of S. similis. Ophiostomapiliferum failed to colonize P. tremuloides wood chips when they were preinoculated with S. similis. Ten known compounds were isolated and identified from the culture filtrate of S. similis. These compounds showed varied fungitoxic effect against O. piliferum at concentrations of 1 to 1000 μg/mL. The potential for S. similis as a biological control agent against O. piliferum on P. tremuloides is discussed.

scholarly journals In vitro propagation of 'Echo' cultivars of Eustoma grandiflorum (Raf.) Shinn.

2005 ◽  
Vol 11 (2) ◽  
Author(s):  
M. Ördögh ◽  
E. Jámbor-Benczúr ◽  
A. Tilly Mándy
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Basal Medium ◽  
The Other ◽  
Eustoma Grandiflorum ◽  
After Effect ◽  
Number Of Shoots

Eustoma grandiflorum (Raf.) Shinn. 'Echo' Fl cultivars ('Echo White', 'Echo Rose', 'Echo Blue', 'Echo Blue Picotee') were used and multiplication of shoots was evaluated on Murashige and Skoog (1962) basal medium with 11 g/1 agar-agar and 20 g/1 sucrose. To test the effect of BA different concentrations were added: 0.10, 0.25 mg/1 and a culture medium without BA. Differentiation of roots was examined on Jámbor-Benczúr and Marta (1990) basal medium with the same concentration of agar-agar and sucrose. To examine the effect on rooting, various concentrations of NAA were used: 0.5, 1.0, 2.0, 3.0 mg/l. The pH was adjusted to 5.6 in every case using KOH. We studied the after-effect of different concentrations of BA during the acclimatisation. During the multiplication, the cultivar 'Echo White' formed the most shoots and the smallest leaves on the medium with 0.10 mg/1 BA. Fortunately, in the case of this cultivar, the number of shoots was reduced and the length of leaves was increased succesfully on the medium without BA. The other three cultivars developed the longest leaves on the medium containing 0.10 mg/1 BA. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. Examining the rooting, the highest percent of roots was found on the medium with 1.0 mg/1 NAA, and the cultivar 'Echo Rose' formed the most roots on this medium. Higher concentration (2.0 and 3.0 mg/1) of NAA already reduced the number of roots in all of the cultivars. During the acclimatisation, the percentage of survival was 76.3% and the tallest plants with the longest leaves were found on the multiplication medium with 0.25 mg/1 BA. 'Echo Blue Picotee' gave the best results with the tallest pieces and longest leaves on this medium.

Sign in / Sign up

Export Citation Format

Share Document