Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.)

1988 ◽  
Vol 12 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Sang-Gu Kim ◽  
Joeng-Rahn Chang ◽  
Hyeon Cheol Cha ◽  
Kwang-Woong Lee
Keyword(s):  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Callus Growth ◽  
Cucumis Sativus L

Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L.

2003 ◽  
Vol 98 (3) ◽  
pp. 213-222 ◽  
Author(s):  
H.G. Ashok Kumar ◽  
H.N. Murthy ◽  
K.Y. Paek
Keyword(s):  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Cucumis Sativus L ◽  
Anther Cultures

scholarly journals Transcriptome analysis of ovary culture-induced embryogenesis in cucumber (Cucumis sativus L.)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12145
Author(s):  
Ying Deng ◽  
Wenyuan Fu ◽  
Bing Tang ◽  
Lian Tao ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Transcriptome Sequencing ◽  
Shoot Formation ◽  
Early Embryo ◽  
Ovary Culture ◽  
Cucumis Sativus L ◽  
Microtubule Binding ◽  
Time Points

Background. Ovary culture is a useful technique used to generate double haploid (DH) cucumber (Cucumis sativus L.) plants. However, cucumber ovary culture have a low rate of embryo induction and plant regeneration. Moreover, the cucumber embryogenesis mechanism remains unclear. In this study, we explored the molecular basis of cucumber embryogenesis in order to establish a foundation for a more efficient ovary culture method. Using transcriptome sequencing, we also investigated the differential expression of genes during the embryogenesis process. Methods. Cytological and morphological observations have divided cucumber ovary culture into three stages: early embryo development (T0), embryo morphogenesis (T1, T2, T3 and T4), and shoot formation (T5). We selected six key time points for transcriptome sequencing and analysis: T0 (the ovules were cultured for 0 d), T1 (the ovules were cultured for 2 d), T2 (the embryos were cultured for 10 d), T3 (the embryos were cultured for 20 d), T4 (the embryos were cultured for 30 d), and T5 (the shoots after 60 d culture). Results. We used cytology and morphology to observe the characteristics of the cucumber’s developmental transformation during embryogenesis and plant regeneration. The differentially expressed genes(DEGs) at developmental transition points were analyzed using transcriptome sequencing. In the early embryogenesis stage, the cells expanded, which was the signal for gametophytes to switch to the sporophyte development pathway. RNA-seq revealed that when compared to the fresh unpollinated ovaries, there were 3,468 up-regulated genes in the embryos, including hormone signal transduction genes, hormone response genes, and stress-induced genes. The reported embryogenesis-related genes BBM, HSP90 and AGL were also actively expressed during this stage. In the embryo morphogenesis stage (from cell division to cotyledon-embryo formation), 480 genes that functioned in protein complex binding, microtubule binding, tetrapyrrole binding, tubulin binding and other microtubule activities were continuously up-regulated during the T1, T2, T3 and T4 time points. This indicated that the cytoskeleton structure was continuously being built and maintained by the action of microtubule-binding proteins and enzyme modification. In the shoot formation stage, 1,383 genes were up-regulated that were mainly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and starch and sucrose metabolism. These up-regualted genes included six transcription factors that contained a B3 domain, nine genes in the AP2/ERF family, and two genes encoding WUS homologous domain proteins. Conclusions. Evaluation of molecular gynogenesis events may contribute to a better understanding of the molecular mechanism of cucumber ovarian culture.

scholarly journals GUS Gene expression and plant regeneration via somatic embryogenesis in cucumber (Cucumis sativus L.)

2008 ◽  
Vol 35 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Hyun-A Kim ◽  
Boo-Youn Lee ◽  
Jin-Jung Jeon ◽  
Dong-Woog Choi ◽  
Pil-Son Choi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Gus Gene ◽  
Cucumis Sativus L ◽  
Gus Gene Expression

Efficient Parthenogenesis Induction and In Vitro Haploid Plant Regeneration in Cucumber (Cucumis sativus L.) Using Putrescine, Spermidine, and Cycocel

2018 ◽  
Vol 37 (4) ◽  
pp. 1127-1134 ◽  
Author(s):  
Hamed Ebrahimzadeh ◽  
Mehran E. Shariatpanahi ◽  
Behzad Ahmadi ◽  
Hassan Soltanloo ◽  
Mahmoud Lotfi ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Haploid Plant ◽  
Cucumis Sativus L

High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of cucumber (Cucumis sativus L.) seedlings

2000 ◽  
Vol 157 (1) ◽  
pp. 136-139 ◽  
Author(s):  
Jae-Whune Kim ◽  
Su-Kyung Han ◽  
Suk-Yoon Kwon ◽  
Haeng-Soon Lee ◽  
Yong Pyo Lim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cucumis Sativus ◽  
High Frequency ◽  
Shoot Induction ◽  
Cucumis Sativus L ◽  
Hypocotyl Explants
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