In vitro micropropagation from nodal segments of Cleistanthus collinus

1996 ◽  
Vol 45 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Afaque Quraishi ◽  
Vijaya Koche ◽  
S. K. Mishra
Keyword(s):  
Nodal Segments ◽  
In Vitro Micropropagation

scholarly journals In Vitro Micropropagation of Stevia rebaudiana Bertoni

2019 ◽  
Vol 29 (2) ◽  
pp. 277-284
Author(s):  
Sabina Yesmin
Keyword(s):  
Morphological Variation ◽  
Stevia Rebaudiana ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Root Induction ◽  
Full Strength ◽  
Stevia Rebaudiana Bertoni ◽  
Regenerated Plants ◽  
In Vitro Micropropagation

Multiple shoots were obtained from both shoot tips and nodal segments cultured on MS fortified with different concentrations of BAP and Kn singly or in combination with low concentration of NAA. Maximum number of shoots (9.28 ± 0.61) were found on MS supplemented with 1.5 mg/l BAP and 05 mg/l NAA. In vitro regenerated shoots were separated and transferred onto half and full-strength MS supplemented with different concentration of IBA, IAA and NAA for root induction. Full strength MS containing 0.2 mg/l IBA was found to be best for root induction where 93.33% shoots were rooted. In vitro regenerated plants grew normally without showing any morphological variation and flowered after 40 days of transplantation.

scholarly journals Artificial induction of tetraploidy in Humulus lupulus L. using oryzalin

2019 ◽  
Vol 72 (2) ◽  
Author(s):  
Michaela Švécarová ◽  
Božena Navrátilová ◽  
Petr Hašler ◽  
Vladan Ondřej
Keyword(s):  
Chromosome Doubling ◽  
Humulus Lupulus ◽  
Nodal Segments ◽  
Root Tips ◽  
Efficient Procedure ◽  
In Vitro Micropropagation ◽  
Humulus Lupulus L ◽  
Artificial Induction ◽  
Internal Standardization

The aim of the research was to establish an efficient procedure for in vitro micropropagation in order to obtain numbers of identical plants for in vitro polyploidization of <em>Humulus lupulus</em> (2<em>n</em> = 20), using antimicrotubule agent oryzalin. For this purpose, the polyploidization was carried out for <em>H. lupulus</em> Osvald’s clones 31, 74, 114, and for ‘Sladek’ cultivar. The two experimental methods – the cultivation of nodal segments on medium with different concentrations of oryzalin (1, 5, and 10 µM) for 2 weeks and the irrigation of nodal segments with oryzalin (10 and 20 µM) for 24 and 48 h were chosen for inducing for polyploid plantlets of <em>H. lupulus</em>. This procedure provided tetraploids, which were identified by flow cytometry using internal standardization method and confirmed using chromosome counting of methaphasic cells from the root tips and morphological observations. The influence of chromosome doubling was also verified with stomata characterization. The polyploid plants were propagated for next evaluation, rooting and transfer to nonsterile conditions and into field. After chromosome doubling, using some different concentration of oryzalin, some plantlets became tetraploids, no mixoploids were detected. The highest efficiency of polyploidization was achieved for clone 72 after 2-week treatment of oryzalin supplemented medium. On the other hand, for method based on the irrigation of nodal segments with oryzalin, the most efficient conditions were treatment with 10 µM and 20 µM oryzalin for 24 and 48 h, respectively.

scholarly journals In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

2018 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Francisca S. Sá ◽  
Jorge M. P. Porto ◽  
Alone L. Brito ◽  
José R. F. Santana ◽  
Rafaeli A. V. Souza ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Indole Acetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Indole Butyric Acid ◽  
In Vitro Plantlets ◽  
In Vitro Micropropagation

This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.

scholarly journals Micropropagation of the Medicinal Plant Eremanthus erythropappus (DC.) MacLeish

HortScience ◽  
2007 ◽  
Vol 42 (6) ◽  
pp. 1420-1424 ◽  
Author(s):  
L.F. Rosal ◽  
J.E.B.P. Pinto ◽  
S.K.V. Bertolucci ◽  
L.C.B. Costa ◽  
R.M. Corrêa
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Shoot Multiplication ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Root Proliferation ◽  
Apical Buds ◽  
In Vitro Micropropagation ◽  
Murashige And Skoog Medium

The aim of the present work was to establish appropriate conditions for the in vitro micropropagation of Eremanthus erythropappus (DC.) MacLeish through shoot multiplication on apical and nodal bud explants. Explants were excised from in vitro-grown seedlings and incubated on Murashige and Skoog medium containing different combinations of 6-benzylaminopurine (BAP) and 1-naphthalene acetic acid (NAA) (for apical buds) and gibberellic acid and NAA (for nodal segments). Proliferation of apical shoots was successfully achieved in the presence of BAP and NAA, each at 1.0 mg L−1, while the elongation of apical shoots could only be attained on medium containing NAA at 1.0 mg L−1. Elongation of nodal shoots was induced in the presence of NAA at 2.0 mg L−1. The most suitable medium for inducing root proliferation on explants of E. erythropappus was NAA at 1.0 mg L−1.

scholarly journals Recent Advances of Biotechnological Tools on Diverse Species of Citrus: Current Applications and Future Prospects

2021 ◽  
pp. 242-265
Author(s):  
Priyanka Sharma ◽  
Bidhan Roy ◽  
Monish Roy
Keyword(s):  
Cost Effective ◽  
Nodal Segments ◽  
Synthetic Seed ◽  
Indigenous Species ◽  
Drought Tolerant ◽  
Diverse Species ◽  
In Vitro Micropropagation ◽  
Cryopreservation Techniques

Based on the long term conservation of several endangered and indigenous species of Citrus, significant impact of biotechnological tools particularly in terms of in-vitro micropropagation methods in addition to synthetic seed production using encapsulation of plant propagules including shoot tips, nodal segments, androgenic embryos, embryogenic callus, etc. in sodium alginate has been highlighted in this manuscript. When seed is not available in enough quantity for raising seedlings for rootstock or have low levels of polyembryony and do not produce adequate quantities of nucellar seedlings, then micropropagation techniques could quickly supply in vitro regenerated rootstock or budwood. Rapid, mass-production and cost-effective biotechnological tools for propagation of citrus rootstocks and budwood would be of great importance in this regard. Reports on another aspect of long term conservation particularly based on storage of cells, tissues and organs of drought tolerant species of Citrus at ultra-low temperature preferably at -196 ºC via applications of cryopreservation techniques using vitrification and encapsulation or dehydration methods has been highlighted in this manuscript. In addition, several research on techniques of in-vitro micrografting using superior scion and rootstocks of two different species of Citrus with an objective of eradication of virus infected citrus stocks for successful production of grafts have been reviewed. Furthermore, effects of explants either through direct and indirect regeneration and conversion into a complete disease free plantlet using suitable synthetic nutrient media along with plant growth regulators at various concentrations and combinations have been highlighted in this manuscript. Hence, the current review is primarily focused on the applications and its effects of superior biotechnological tools for long term conservation of diverse species of citrus for further increasing the potentiality of Citrus industries in addition to genetic improvement and genetic resource conservation.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals Influence of Cytokinins in Combination with GA3on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis(L.) O. Kuntze)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Reza Azadi Gonbad ◽  
Uma Rani Sinniah ◽  
Maheran Abdul Aziz ◽  
Rosfarizan Mohamad
Keyword(s):  
Camellia Sinensis ◽  
Woody Plants ◽  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Liquid Form ◽  
Solid Media

The use ofin vitroculture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis(L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

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