Antisense suppression of skeletal muscle myosin light chain-1 biosynthesis impairs myofibrillogenesis in cultured myotubes

1995 ◽  
Vol 16 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Ralph Nawrotzki ◽  
Donald A. Fischman ◽  
Takashi Mikawa
Keyword(s):  
Skeletal Muscle ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Skeletal Muscle Myosin ◽  
Antisense Suppression ◽  
Cultured Myotubes ◽  
Muscle Myosin

Shape and substructure of skeletal muscle myosin light chain kinase

Biochemistry ◽  
1983 ◽  
Vol 22 (18) ◽  
pp. 4316-4326 ◽  
Author(s):  
Georg W. Mayr ◽  
Ludwig M. G. Heilmeyer
Keyword(s):  
Skeletal Muscle ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

Molecular characterization of rat skeletal muscle myosin light chain kinase

1989 ◽  
Vol 256 (2) ◽  
pp. C399-C404 ◽  
Author(s):  
B. P. Herring ◽  
M. H. Nunnally ◽  
P. J. Gallagher ◽  
J. T. Stull
Keyword(s):  
Skeletal Muscle ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Skeletal Muscles ◽  
Myosin Light Chain ◽  
Cardiac Tissue ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Myosin ◽  
Slow Twitch ◽  
Muscle Myosin

A 1.85-kilobase (kb) cDNA has been isolated that encodes the catalytic and calmodulin binding domains of rat skeletal muscle myosin light chain kinase. The cDNA hybridized to a 3.3-kb RNA present in fast- and slow-twitch skeletal muscles. The reported enzymatic activity (3-fold greater in fast- than slow-twitch skeletal muscles) reflects the relative abundance of this RNA in the two types of skeletal muscle. No hybridization of the cDNA was detected to RNA isolated from smooth or nonmuscle tissues. The clone cross hybridized to a 2.2-kb RNA present in cardiac tissue. Ribonuclease protection analysis of skeletal and cardiac muscle RNA revealed major differences in the two hybridizing RNAs. Thus rat skeletal muscle contains a single myosin light chain kinase isoform, which is distinct from the cardiac, smooth, and nonmuscle forms.

Sign in / Sign up

Export Citation Format

Share Document