Performance of a tubular microporous membrane filter used for in situ sampling of mammalian cell culture medium

1991 ◽  
Vol 5 (3) ◽  
pp. 183-186 ◽  
Author(s):  
H. Graf ◽  
D. Wentz ◽  
K. Sch�gerl
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Culture Medium ◽  
Membrane Filter ◽  
Mammalian Cell Culture ◽  
Microporous Membrane ◽  
Cell Culture Medium

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium

Development of an Assay for the Measurement of the Surfactant Pluronic F-68 in Mammalian Cell Culture Medium

1998 ◽  
Vol 262 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Hazem Ghebeh ◽  
Anita Handa-Corrigan ◽  
Michael Butler
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Cell Culture Medium

Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127

Biomaterials ◽  
2002 ◽  
Vol 23 (23) ◽  
pp. 4615-4619 ◽  
Author(s):  
Julie E Matthew ◽  
Yesenia L Nazario ◽  
Susan C Roberts ◽  
Surita R Bhatia
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Cell Culture Medium

scholarly journals Combination of nutrients in a mammalian cell culture medium kills cryptococci

2018 ◽  
Vol 57 (3) ◽  
pp. 374-383 ◽  
Author(s):  
Donald L Granger ◽  
Donna M Call
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Cell Culture Medium

Experiments in Nanomechanical Properties of Live Osteoblast Cells and Cell–Biomaterial Interface

2011 ◽  
Vol 2 (4) ◽  
Author(s):  
Rohit Khanna ◽  
Kalpana S. Katti ◽  
Dinesh R. Katti
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Structure ◽  
Living Cells ◽  
Actin Stress Fiber ◽  
Cell Culture Medium ◽  
Nanomechanical Properties ◽  
Cell Substrate ◽  
Significant Difference

Characterizing the mechanical characteristics of living cells and cell–biomaterial composite is an important area of research in bone tissue engineering. In this work, an in situ displacement-controlled nanoindentation technique (using Hysitron Triboscope) is developed to perform nanomechanical characterization of living cells (human osteoblasts) and cell–substrate constructs under physiological conditions (cell culture medium; 37 °C). In situ elastic moduli (E) of adsorbed proteins on tissue culture polystyrene (TCPS) under cell culture media were found to be ∼4 GPa as revealed by modulus mapping experiments. The TCPS substrates soaked in cell culture medium showed significant difference in surface nanomechanical properties (up to depths of ∼12 nm) as compared to properties obtained from deeper indentations. Atomic force microscopy (AFM) revealed the cytoskeleton structures such as actin stress fiber networks on flat cells which are believed to impart the structural integrity to cell structure. Load-deformation response of cell was found to be purely elastic in nature, i.e., cell recovers its shape on unloading as indicated by linear loading and unloading curves obtained at 1000 nm indentation depth. The elastic response of cells is obtained during initial cell adhesion (ECell, 1 h, 1000 nm = 4.4–12.4 MPa), cell division (ECell, 2 days, 1000 nm = 1.3–3.0 MPa), and cell spreading (ECell, 2 days, 1000 nm = 6.9–11.6 MPa). Composite nanomechanical responses of cell–TCPS constructs were obtained by indentation at depths of 2000 nm and 3000 nm on cell-seeded TCPS. Elastic properties of cell–substrate composites were mostly dominated by stiff TCPS (EBulk = 5 GPa) lying underneath the cell.

Properties of a fungicidal product formed from a reaction between L-cystine and pyridoxal

2020 ◽  
Vol 58 (7) ◽  
pp. 919-927
Author(s):  
Elizabeth A Johnston ◽  
Spencer B Lloyd ◽  
Donald L Granger
Keyword(s):  
Cell Culture ◽  
Schiff Base ◽  
Culture Medium ◽  
Fungicidal Activity ◽  
Mammalian Cell Culture ◽  
Wave Length ◽  
Aldehyde Group ◽  
Cell Culture Medium ◽  
Absorbance Maximum ◽  
Three Components

Abstract Previously we found that three components of a commonly used mammalian cell culture medium incorporated into agar killed cryptococci (Granger and Call 2019). The components were L-cystine, iron [Fe(III)], and pyridoxal (CIP). We now report on a buffered solution at neutral pH of the three components, which was highly fungicidal without agar. We showed that CIP fungicidal activity, identical to the findings with cell culture medium, was inactivated by visible light and was unstable with storage in the dark. Congeners replacing either pyridoxal or L-cystine in CIP revealed structural requirements for fungicidal activity. Replacing pyridoxal in CIP with 2-hydroxy-5-nitrobenzaldehyde produced a solution that was equally fungicidal and maintained fungicidal activity upon storage in the dark for up to 50 days. We employed methods for excluding iron from CIP and found that fungicidal activity was not affected. Upon mixing L-cystine and pyridoxal in buffer at pH 7.0, diode array spectroscopy revealed a red-shift of absorbance maximum from 391 nm to 398 nm. Our findings point to Schiff base reaction between the pyridoxal aldehyde group of C1 with the alpha amino group(s) of cystine to yield a fungicidal compound. Light at wave length approximately 400 nm inactivates this complex accompanied by bleaching of the pyridine ring of pyridoxal. Our findings may be useful for design of a class of fungicidal compounds formed through Schiff base reaction of disulfide compounds with aromatic ring-bearing aldehydes.

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