Absorbed substrate fermentation for pectinase production with Aspergillus niger

1994 ◽  
Vol 8 (11) ◽  
pp. 837-842 ◽  
Author(s):  
S. Huerta ◽  
E. Favela ◽  
R. L�pez-Ulibarri ◽  
A. Fonseca ◽  
G. Viniegra-Gonz�lez ◽  
...  
2013 ◽  
Vol 48 (1) ◽  
pp. 25-32 ◽  
Author(s):  
S Islam ◽  
B Feroza ◽  
AKMR Alam ◽  
S Begum

Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity. Moisture content did not affect the activity significantly. Media composition was varied to optimize the enzyme production in solid state fermentation. It was observed that the highest pectinase activity of 831.0 U/g was found to produce in presence of yeast extract as a nitrogen source in combination with ammonium sulfate in assay media. Aeration showed positive significant effects on pectinase production 755 U/g at 1000 ml flasks. The highest pectinase production was found at 2 g pectin (521 U/g) used as a substrate. Pectinolytic activity was found to have undergone catabolite repression with higher pectin concentration (205 U/g at 5 g pectin). The incubation period to achieve maximum pectinase activity by the isolated strain Aspergillus niger IM09 was 3 days, which is suitable from the commercial point of view. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15410 Bangladesh J. Sci. Ind. Res. 48(1), 25-32, 2013


2006 ◽  
Vol 64 (2) ◽  
pp. 244-250 ◽  
Author(s):  
Jing Debing ◽  
Li Peijun ◽  
Frank Stagnitti ◽  
Xiong Xianzhe ◽  
Ling Li

1999 ◽  
Vol 25 (1-2) ◽  
pp. 103-108 ◽  
Author(s):  
Octavio Loera ◽  
Jesús Aguirre ◽  
Gustavo Viniegra-González

2018 ◽  
Author(s):  
J. C. ROCHA ◽  
J. S. ARAÚJO ◽  
W. K. V. PAIVA ◽  
C. R. R. FERREIRA ◽  
M. G. F. SILVA ◽  
...  

Author(s):  
SB Oyeleke ◽  
OA Oyewole ◽  
EC Egwim ◽  
BEN Dauda ◽  
EN Ibeh

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Hesham A. El Enshasy ◽  
Elsayed Ahmed Elsayed ◽  
Noorhamizah Suhaimi ◽  
Roslinda Abd Malek ◽  
Mona Esawy

2020 ◽  
Vol 4 (2) ◽  
pp. 244-249
Author(s):  
Abubakar Madika ◽  
Ulem Asagha Eugene ◽  
Musa Bishir ◽  
Mamunu Abdulkadir Sulaiman ◽  
Ibrahim Mohammed Hussaini

This study was conducted to screen for pectinase production by Aspergillus niger isolated from soil samples collected from three different locations within Ahmadu Bello University, Zaria, namely; botanical garden, refuse dump and sheep pen sites. A total of fifteen (15) soil samples were collected from different locations and used for isolation by a cultural method. Isolates suspected to be Aspergillus niger were further identified by microscopic examination of the lactophenol cotton blue stained-preparation and slide culture technique. The isolates were then screened in a pectin-containing medium for their pectinase activity. The isolates were further subjected to pectinase production using citrus pectin as the substrate under submerged fermentation conditions. Seven (7) isolates were confirmed to be Aspergillus niger with percentage occurrence of 60% each from sheep pen (SP) and refuse dumpsites (RD), and 20% from the botanical garden (BG). Aspergillus niger RD3 produced the highest zone of pectin hydrolysis (53 ± 1.1 mm) while isolate RD5 produced the lowest (35 ± 3.1 mm). Under submerged fermentation conditions, Aspergillus niger SP5 had the highest pectinase activity of 2.92 U/mL while isolate RD4 had the lowest pectinase activity of 1.29 U/mL. Aspergillus niger can be readily isolated from various soil types with the highest frequency in soils from sheep pen and refuse dumpsites. All the Aspergillus niger isolates demonstrated the potential for pectinase production. The study reveals the potential of various Aspergillus niger isolates from different soil in the production of pectinase.


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