A novel design of solid state fermenter and its evaluation for cellulase production by Trichoderma viride SL-1

In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. Copyright © 2018 BCREC Group. All rights reservedReceived: 14th December 2017; Revised:29th July 2018; Accepted: 3rd August 2018How to Cite: Wonoputri, V., Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R. (2018). Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 553-559 (doi:10.9767/bcrec.13.3.1964.553-559)Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.1964.553-559

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Bioprocessing of Areca husk in solid state fermentation for cellulase production using Trichoderma viride

Indian Journal Of Applied Research ◽

10.15373/2249555x/dec2011/6 ◽

2011 ◽

Vol 1 (3) ◽

pp. 15-17

Author(s):

Bhagyamma CV Bhagyamma CV ◽

◽

Sharad S Sharad S ◽

Shriganesh G Gudigar ◽

Reshma S.V Reshma S.V

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Trichoderma Viride ◽

Cellulase Production

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Production and Optimization of Cellulase Enzyme Using Aspergillus niger USM AI 1 and Comparison with Trichoderma reesei via Solid State Fermentation System

Biotechnology Research International ◽

10.4061/2011/658493 ◽

2011 ◽

Vol 2011 ◽

pp. 1-6 ◽

Cited By ~ 26

Author(s):

C. K. Lee ◽

I. Darah ◽

C. O. Ibrahim

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Trichoderma Reesei ◽

Palm Kernel ◽

Cellulase Production ◽

Palm Kernel Cake ◽

Fermentation System ◽

Production Studies ◽

Novel Design ◽

Filter Paper Activity

Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30∘C; aeration at 4 L/h·g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 U/g of Filter paper activity (FPase) was obtained. At the same time, comparative studies of the enzymes production under the same SSF conditions indicated that FPase produced by A. niger USM AI 1 was about 35.3% higher compared to Trichoderma reesei. This shows that the performance of this newly designed SSF bioreactor is acceptable and potentially used as prototype for larger-scale bioreactor design.

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Optimization of Cellulase Production Using Agricultural Wastes by Artificial Neural Network and Genetic Algorithm

Chemical Product and Process Modeling ◽

10.2202/1934-2659.1553 ◽

2011 ◽

Vol 6 (1) ◽

Cited By ~ 4

Author(s):

Chun Chang ◽

Guizhuan Xu ◽

Junfang Yang ◽

Duo Wang

Keyword(s):

Neural Network ◽

Genetic Algorithm ◽

Artificial Neural Network ◽

Solid State ◽

Aspergillus Niger ◽

Moisture Content ◽

Trichoderma Viride ◽

Cellulase Activity ◽

Cellulase Production ◽

Fermentation Time

The cellulase production by Trichoderma viride was optimized using artificial intelligence-based techniques under solid state fermentation. In this study, a back propagation network was designed with Levenberg-Marquardt training algorithm, and the tangent sigmoid and pure linear functions were used as the transfer functions in the hidden and output layers of the ANN, respectively. An artificial neural network coupling genetic algorithms was used to optimize the process parameters, which include the mass ratio of wheat straw to wheat bran, moisture content and fermentation time. The ultimate process parameters of optimization were mass ration of wheat straw to wheat bran 2.9, moisture content 69.6 percent, and fermentation time 123.3h. Further test experiment showed that the final cellulase activity can reach to 11.62 U/g, which was the highest value among all the experimental results. This result indicates that the genetic algorithm based on a neural network model is a better optimization method for cellulase production in solid state fermentation. To improve the cellulase production, a mixed culture system of Trichoderma viride and Aspergillus niger was also developed. The cellulase activity increased by 7.40 percent with the addition of Aspergillus niger at 72h.

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Research on Conditions of Producing Cellulose by Mixed Fermentation for Bioethanol

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.383-390.3986 ◽

2011 ◽

Vol 383-390 ◽

pp. 3986-3991

Author(s):

Xiang Ping Su ◽

Da Chun Gong ◽

Xiao Li ◽

Guo Hua Chen

Keyword(s):

Enzyme Activity ◽

Solid State ◽

Trichoderma Viride ◽

Cellulase Production ◽

Cultivation Condition ◽

Mass Ratio ◽

Single Factor ◽

Mixed Fermentation ◽

Fermentation Time ◽

Orthogonal Experiments

The conditions for cellulase production were studied with the mixed germs of Aspergillus niger (A.niger) and Trichoderma viride (R.viride) using straw stalk powder as carbon recourse by solid-state fermentation (SSF). The conditions of the mixed strains SSF were optimized by single factor experiments and orthogonal experiments in four aspects: the inoculation ratio, the bran-straw mass ratio, fermentation time and the filled volume. The optimal SSF conditions of the mixed strains were as follows: inoculation ratio of R.viride to A.niger was 1, the bran-straw mass ratio was 0.3, the time of fermentation was four days and the filled volume is 100 mL /1000 mL. The enzyme activity of FPA, CMC and β-glucosidase are 5.29 IU/mL, 9.33 IU/mL and 49.91 IU/mL in the optimal cultivation condition, which were 2.28~2.47 times, 2.39~2.45 times, 1.38~2.09 times as high as single-germ fermentation, respectively. The enzyme activity of cellulose from the mixed fermentation was higher than that from the fermentation of single bacteria.

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Nutritional composition of maize husk silage generated from solid state fermentation by Trichoderma viride UP01

Pakistan Journal of Botany ◽

10.30848/pjb2019-6(28) ◽

2019 ◽

Vol 51 (6) ◽

Author(s):

Thana Somchart ◽

Anorach Tosawat ◽

Sorachakula Choke ◽

Danmek Khanchai

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Trichoderma Viride ◽

Nutritional Composition

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PRODUCTION OF LIGNOCELLULOSES FROM ASPERGILLUS NIGER USING ARECA NUT SPADE AS BIOMASS..

10.36106/ijsr/1515973 ◽

2019 ◽

pp. 1-3

Author(s):

Madhuri B ◽

Narasimha G ◽

Balaji M*

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Cellulase Production ◽

Areca Nut ◽

Physical Treatment ◽

Cellulose Production ◽

Lactobionic Acid ◽

Cellulase Enzyme

Areca palm (ChrysalidoCarpus lutescenes) a widely used plant having feathery arching brands with 100 leaflets. All these plants produce much of waste in additions to greeny and nuts. This waste of spade is used for the production of various molecules that are used in industry and pharma sector. Fermentation techniques are used to generate economically important enzymes for industrial and pharmaceutical purposes. Cellulase enzyme degrades the cellulose in between β-1, 4 glucosidic link found in lignocellulosic complex which under physical treatment is slower to degrade. The present study of Aspergillus niger for cellulose production was carried in solid state (SS) and submerged (SM) fermentations for production of cellulase enzyme. Cellulase production in SSF after 72 h of fermentation was 8.02 and in SMF activity was 2.98 per ml of cultured broth at H 6 and temperature at 30°C. Both SMF and SSF were supplemented with lactose and lactobionic acid, which acted as cellulase P production inducers. The aim of the present work was to study the effect of Areca palm spade as substrate for Aspergillus niger and its cellulase production under SMF and SSF.

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