Research on Conditions of Producing Cellulose by Mixed Fermentation for Bioethanol

2011 ◽  
Vol 383-390 ◽  
pp. 3986-3991
Author(s):  
Xiang Ping Su ◽  
Da Chun Gong ◽  
Xiao Li ◽  
Guo Hua Chen
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Trichoderma Viride ◽  
Cellulase Production ◽  
Cultivation Condition ◽  
Mass Ratio ◽  
Single Factor ◽  
Mixed Fermentation ◽  
Fermentation Time ◽  
Orthogonal Experiments

The conditions for cellulase production were studied with the mixed germs of Aspergillus niger (A.niger) and Trichoderma viride (R.viride) using straw stalk powder as carbon recourse by solid-state fermentation (SSF). The conditions of the mixed strains SSF were optimized by single factor experiments and orthogonal experiments in four aspects: the inoculation ratio, the bran-straw mass ratio, fermentation time and the filled volume. The optimal SSF conditions of the mixed strains were as follows: inoculation ratio of R.viride to A.niger was 1, the bran-straw mass ratio was 0.3, the time of fermentation was four days and the filled volume is 100 mL /1000 mL. The enzyme activity of FPA, CMC and β-glucosidase are 5.29 IU/mL, 9.33 IU/mL and 49.91 IU/mL in the optimal cultivation condition, which were 2.28~2.47 times, 2.39~2.45 times, 1.38~2.09 times as high as single-germ fermentation, respectively. The enzyme activity of cellulose from the mixed fermentation was higher than that from the fermentation of single bacteria.

Optimization of Cellulase Production Using Agricultural Wastes by Artificial Neural Network and Genetic Algorithm

2011 ◽  
Vol 6 (1) ◽  
Author(s):  
Chun Chang ◽  
Guizhuan Xu ◽  
Junfang Yang ◽  
Duo Wang
Keyword(s):  
Neural Network ◽  
Genetic Algorithm ◽  
Artificial Neural Network ◽  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Trichoderma Viride ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Fermentation Time

The cellulase production by Trichoderma viride was optimized using artificial intelligence-based techniques under solid state fermentation. In this study, a back propagation network was designed with Levenberg-Marquardt training algorithm, and the tangent sigmoid and pure linear functions were used as the transfer functions in the hidden and output layers of the ANN, respectively. An artificial neural network coupling genetic algorithms was used to optimize the process parameters, which include the mass ratio of wheat straw to wheat bran, moisture content and fermentation time. The ultimate process parameters of optimization were mass ration of wheat straw to wheat bran 2.9, moisture content 69.6 percent, and fermentation time 123.3h. Further test experiment showed that the final cellulase activity can reach to 11.62 U/g, which was the highest value among all the experimental results. This result indicates that the genetic algorithm based on a neural network model is a better optimization method for cellulase production in solid state fermentation. To improve the cellulase production, a mixed culture system of Trichoderma viride and Aspergillus niger was also developed. The cellulase activity increased by 7.40 percent with the addition of Aspergillus niger at 72h.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

scholarly journals Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch

2018 ◽  
Vol 13 (3) ◽  
pp. 553
Author(s):  
Vita Wonoputri ◽  
Subiantoro Subiantoro ◽  
Made Tri Ari Penia Kresnowati ◽  
Ronny Purwadi
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Trichoderma Viride ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Contact Method ◽  
Empty Fruit Bunch ◽  
Liquid Ratio ◽  
Fermentation Parameters

In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. Copyright © 2018 BCREC Group. All rights reservedReceived: 14th December 2017; Revised:29th July 2018; Accepted: 3rd August 2018How to Cite: Wonoputri, V., Subiantoro, S., Kresnowati, M.T.A.P., Purwadi, R. (2018). Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch. Bulletin of Chemical Reaction Engineering & Catalysis, 13 (3): 553-559 (doi:10.9767/bcrec.13.3.1964.553-559)Permalink/DOI: https://doi.org/10.9767/bcrec.13.3.1964.553-559 

Bioprocessing of Areca husk in solid state fermentation for cellulase production using Trichoderma viride

2011 ◽  
Vol 1 (3) ◽  
pp. 15-17
Author(s):  
Bhagyamma CV Bhagyamma CV ◽  
◽  
Sharad S Sharad S ◽  
Shriganesh G Gudigar ◽  
Reshma S.V Reshma S.V
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Trichoderma Viride ◽  
Cellulase Production

Study on Calcination Process of Serpentine Mixed with Ammonium Sulfate

2013 ◽  
Vol 320 ◽  
pp. 296-302 ◽  
Author(s):  
Bei Song ◽  
Yu Ran Zhang ◽  
Yong Hao Di ◽  
Rui Dai ◽  
Shui Lin Zheng
Keyword(s):  
Ammonium Sulfate ◽  
Sem Analysis ◽  
Mass Ratio ◽  
Single Factor ◽  
Water Leaching ◽  
Filtration Process ◽  
Calcination Process ◽  
Orthogonal Experiments ◽  
Leaching Residue ◽  
Calcination Duration

The research involves the calcination of QingHaiQiLian serpentine with ammonium sulfate in order to extract magnesium, and evaluates calcination results through the mass of water leaching residue that comes from the calcination, dissolution and filtration process. Optimal calcination parameters of serpentine and ammonium sulfate determined by orthogonal experiments, single-factor experiments, elemental analysis and SEM analysis are: calcination duration of 1h, calcination temperature of 700°C, ammonium sulfate dosage of 90g (or mass ratio of ammonium sulfate to serpentine sample (m1/m2) of 3:2), under which condition a magnesium extraction rate of 62.75% can be achieved.

Optimization Research on Buckwheat Vinegar Fermentation Process

2014 ◽  
Vol 651-653 ◽  
pp. 265-268
Author(s):  
Ting Jun Ma ◽  
Na Xi A Yi ◽  
Xiu Shi Yang ◽  
Pei You Qin ◽  
San Cai Liu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Submerged Fermentation ◽  
Conversion Rate ◽  
Fermentation Process ◽  
Production Cycle ◽  
Single Factor ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Orthogonal Experiments ◽  
Active Substances

Buckwheat is rich in active substances with hypoglycemic effects. Submerged fermentation, as the method of brewing buckwheat vinegar, not only has the advantages of shortening production cycle, saving materials etc, but also has high functional active substances. In this study, the process of the submerged fermentation of acetic acid fermentation was optimized by the orthogonal experiments of L9 (34), which was also on the basis of single factor experiments. The results indicated that the conversion rate of acetic acid can become 95.8% when the initial alcohol at 6% (v / v), inoculum at 10%, fermentation temperature 30°Cand fermentation time 60 h.

scholarly journals Ozonation Pretreatment Evaluation for Xylanase Crude Extract Production from Corncob under Solid-State Fermentation

2017 ◽  
Vol 1 (2) ◽  
pp. 27 ◽  
Author(s):  
Efri Mardawati ◽  
Surya Martha Pratiwi ◽  
Robi Andoyo ◽  
Tita Rialita ◽  
Mochamad Djali ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Solid State Fermentation ◽  
Chemical Industry ◽  
Crude Extract ◽  
Incubation Time ◽  
Trichoderma Viride ◽  
Xylanase Activity ◽  
Alternative Substrate ◽  
Xylanase Production

Xylanases are highly exploited enzyme in industries, including food and chemical industry. Xylanases can be utilized in catalyzing the endohydrolysis of 1,4-β-xylosidic linkages in xylan, lignocellulosic component to produce xylose-monomer. This research aims to optimize xylanase production from alternative substrate, corncob. Corncob contains 41.17% of hemicellulose, polymer of xylan. Xylanases are produced through solid state fermentation by Trichoderma viride. Ratio between substrate and moistening solution was 0.63 g/mL with fermentation temperature 32,8OC. Variables varied include incubation time and pretreatment (using autoclave, ozonation, combination of ozonation and autoclave, also without pretreatment). Xylanase activity was measured by DNS method using 1% of xylan as substrate standard. The result showed that the best incubation time is 36 h with 14403.8707 U/mg protein for specific xylanase activity by using autoclave as pretreatment.  Ozonation pretreatment process can increase the enzyme activity of xylanase.

Optimization of Fermentation Conditions for Higher Monacolin K Production by Monascus purpureus

2013 ◽  
Vol 781-784 ◽  
pp. 1397-1402 ◽  
Author(s):  
Li Juan Yu ◽  
Hong Xing Zhang ◽  
Yuan Hong Xie ◽  
Si Ming Ma ◽  
Hui Liu ◽  
...  
Keyword(s):  
Solid State ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Monascus Purpureus ◽  
Monacolin K ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Orthogonal Experiments ◽  
Optimization Of Fermentation ◽  
Optimized Conditions

Monacolin K is a secondary metabolite of Monascus fermentation with lowering blood fat, cholesterol and blood sugar. The culture substrates and fermentation conditions were optimized by means of L9 (33) orthogonal experiments. The optimized fermentation conditions were studied and concluded: initial moisture content 50%, material thickness 4 cm and fermentation time 13 days. Using rice as substrate, the maximum yield of monacolin K was 2.50 mg/g in the optimized conditions. Using glutinousrice as substrate, the maximum yield of monacolin K was 2.71 mg/g in the optimized conditions. The founding determined that glutinousrice was used as substrate in solid state fermentation by Monascus purpureus to produce monacolinK.

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