In vitro pollen maturation and successful seed production in detached spikelet cultures in wheat (Triticum aestivum L.)

1992 ◽  
Vol 5 (4) ◽  
Author(s):  
B. Barnab�s ◽  
G. Kov�cs
Keyword(s):  
Triticum Aestivum ◽  
Seed Production ◽  
Triticum Aestivum L ◽  
Pollen Maturation

Comparison of in vitro androgenic response and F1 seed production in wheat (Triticum aestivum L.)

2001 ◽  
Vol 29 (3-4) ◽  
pp. 297-304
Author(s):  
M. Hasan ◽  
J. Pauk ◽  
L. Jankulosky ◽  
R. Mihály ◽  
K. Tóth-Lőkös ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Seed Production ◽  
Triticum Aestivum L

HAPLOID PLANTS (2n = 21) FROM in vitro ANTHER CULTURE OF TRITICUM AESTIVUM

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Haploid Plants

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).

scholarly journals Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Bread Wheat ◽  
Binary Vector ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Pcr Analysis ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

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