Immunohistochemical localization of the luteinizing hormone releasing hormone (LHRH)-containing structures in the central nervous system of the domestic fowl

1982 ◽  
Vol 227 (2) ◽  
pp. 451-458 ◽  
Author(s):  
Rita J�zsa ◽  
B. Mess

1983 ◽  
Vol 31 (1) ◽  
pp. 62-68 ◽  
Author(s):  
J C King ◽  
R M Lechan ◽  
G Kugel ◽  
E L Anthony

Acrolein was examined as an alternative fixative to formaldehyde for immunocytochemical localization of neuropeptides in the rat brain. A brief (5 min) vascular perfusion with a 5% acrolein solution allowed the identification of thyrotropin-releasing hormone (TRH), vasoactive intestinal peptide (VIP), somatostatin (SRIF), neurotensin (NT), methionine enkephalin (Menk), adrenocorticotropic hormone (ACTH), tyrosine hydroxylase (TH), and luteinizing hormone-releasing hormone (LHRH) in fibers and perikarya within the central nervous system of the rat using the peroxidase-antiperoxidase (PAP) technique. Acrolein appears to be particularly valuable for immunocytochemistry, as it 1) stabilizes heterogeneous peptides and proteins rapidly and effectively, 2) retains antigenicity, and 3) preserves morphological detail.



1983 ◽  
Vol 4 (2) ◽  
pp. 161-168 ◽  
Author(s):  
YASUHIKO IBATA ◽  
HITOSHI OKAMURA ◽  
KENJI FUKUI ◽  
HIROKO L. OBATATSUTO ◽  
MINORU TANAKA ◽  
...  


1978 ◽  
Vol 7 (1) ◽  
pp. 49-53 ◽  
Author(s):  
E.C. Griffiths ◽  
R. Forbes ◽  
S.L. Jeffcoate ◽  
D.T. Holland




1981 ◽  
Vol 96 (3) ◽  
pp. 394-397 ◽  
Author(s):  
Jau-Nan Lee ◽  
Markku Seppälä ◽  
Tim Chard

Abstract. High pressure liquid chromatography (HPLC) and radioimmunoassay were employed to characterize luteinizing hormone-releasing factor (LRF)-like material in the human placenta. Methanol extracts of the placenta were washed with acetic acid and chloroform, further purified on coarse octadecylsilane columns, fractionated on HPLC, and tested by radioimmunoassay. In HPLC, placental LRF had the same retention time as synthetic LRF, and such fractions gave an inhibition curve which was parallel to that of synthetic LRF in radioimmunoassav. It is concluded that human placental I.RF is similar or identical to LRF in the central nervous system.





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