Influence of changes in composition of the cerebrospinal fluid on the secretory activity of the subcommissural organ in Rana esculenta

1977 ◽  
Vol 185 (4) ◽  
Author(s):  
J. Hess ◽  
J.H.B. Diederen ◽  
H.G.B. Vullings
Keyword(s):  
Cerebrospinal Fluid ◽  
Subcommissural Organ ◽  
Secretory Activity ◽  
Rana Esculenta

A new sex dimorphism in the Harderian gland of the frog Rana esculenta

2007 ◽  
Vol 85 (8) ◽  
pp. 909-915 ◽  
Author(s):  
Ismene Serino ◽  
Gaia Izzo ◽  
Diana Ferrara ◽  
Michela d’Istria ◽  
Sergio Minucci
Keyword(s):  
Sexual Dimorphism ◽  
Secretory Activity ◽  
Harderian Gland ◽  
Polymerase Chain Reaction Analysis ◽  
Rana Esculenta ◽  
Rt Pcr ◽  
Polymerase Chain ◽  
Two Peaks ◽  
Constant Expression

The Harderian gland (Hg), the only gland found in the orbit of the frog Rana esculenta L., 1758, probably plays a role in orbital lubrication. The secretory activity of the Hg is seasonal, showing the highest activity in summer. There is little information on Hg gene expression; previously, we identified a mRNA named harderin, whose deduced protein has no homology with other proteins. Differential expression of the harderin transcript between the sexes expressed during the annual cycle implies sexual dimorphism. RT–PCR (reverse transcription – polymerase chain reaction) analysis, revealed that harderin is expressed during the entire year in the Hg of both sexes. It shows a higher level of expression in the female glands than that of male glands. Two peaks of expression, in February and in June, were observed in the female glands, while only the February peak was observed in those of males. These observations were supported by in situ hybridization. Experiments involving gonadectomy and (or) hormonal replacement therapy showed a significant decrease in harderin in the Hg of females; this effect is prevented by estradiol (testosterone had no effect), while ICI (antiestrogen) counteracts the hormonal prevention, suggesting that this sexual dimorphism is under estradiol control. The constant expression of harderin mRNA during the year suggests a probable constitutive role for this molecule.

Subcommissural organ, cerebrospinal fluid circulation, and hydrocephalus

2001 ◽  
Vol 52 (5) ◽  
pp. 591-607 ◽  
Author(s):  
Jos� Manuel P�rez-F�gares ◽  
Antonio J. Jimenez ◽  
Esteban M. Rodr�guez
Keyword(s):  
Cerebrospinal Fluid ◽  
Subcommissural Organ ◽  
Fluid Circulation ◽  
Cerebrospinal Fluid Circulation

Changes in the cerebrospinal-fluid monoamines in rats with an immunoneutralization of the subcommissural organ-Reissner's fiber complex by maternal delivery of antibodies

1999 ◽  
Vol 128 (3) ◽  
pp. 278-290 ◽  
Author(s):  
Sara Rodríguez ◽  
Karin Vio ◽  
Carolina Wagner ◽  
Miguel Barría ◽  
Edison H. Navarrete ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Subcommissural Organ ◽  
Reissner's Fiber ◽  
Reissner’S Fiber

Secretory activity and serotonin innervation of subcommissural organ

Neuroreport ◽  
2000 ◽  
Vol 11 (9) ◽  
pp. 1821-1825 ◽  
Author(s):  
Samir Ahboucha ◽  
Marianne Didler-Bazes ◽  
Annie Meiniel ◽  
Michelle Fèvre-Montange ◽  
Halima Gamrani
Keyword(s):  
Subcommissural Organ ◽  
Secretory Activity

Benzodiazepine receptors and cerebrospinal fluid formation

1990 ◽  
Vol 72 (5) ◽  
pp. 759-762 ◽  
Author(s):  
Gregg L. Williams ◽  
Michael Pollay ◽  
Thomas Seale ◽  
Brent Hisey ◽  
P. Alex Roberts
Keyword(s):  
Cerebrospinal Fluid ◽  
Choroid Plexus ◽  
Binding Sites ◽  
Benzodiazepine Receptors ◽  
Secretory Activity ◽  
Assay Method ◽  
Content Type ◽  
High Affinity ◽  
Fluid Formation

✓ There is autoradiographic evidence that peripheral-type benzodiazepine ligands bind with high affinity to the membranes of choroid plexus tissue. In this study, the binding of a 4′-chloro analog of diazepam (Ro 5-4864) to rabbit choroid plexus and cerebral cortex was accomplished utilizing an in vitro radioactive assay method. A kinetic analysis of this binding revealed a relatively high affinity of this ligand (KD) for peripheral binding sites in plexus tissue (KD = 16.1 nM/mg protein). There was a 4.6-fold greater density of binding sites (total receptor density (Bmax) = 2.3 pmol/mg) in choroidal membrane as compared to cortical tissue (Bmax = 0.5 pmol/mg). In 40 rabbits in which a ventricular perfusion system was used, the rate of cerebrospinal fluid (CSF) formation was observed to decrease some 48% in the presence of 10−4 M Ro 5-4864, although some inhibition of secretory activity was still noted at a CSF concentration of 10−8 M. The choroid plexus tissue levels of adenosine 3′,5′cyclic monophosphate (cAMP) and adenosine triphosphatase (ATPase) were not affected by 10−4 M Ro 5-4864. The results of this study support the notion that the specific benzodiazepine peripheral binding sites in choroid plexus serve to modulate CSF formation. The mechanism of action is poorly understood but does not involve the transport ATPase system or the second messenger cAMP.

Androgen receptor in the Harderian gland of Rana esculenta

1991 ◽  
Vol 129 (2) ◽  
pp. 227-232 ◽  
Author(s):  
M. d'Istria ◽  
G. Chieffi-Baccari ◽  
L. Di Matteo ◽  
S. Minucci ◽  
B. Varriale ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Binding Capacity ◽  
Specific Binding ◽  
Secretory Activity ◽  
Harderian Gland ◽  
Nuclear Extract ◽  
Rana Esculenta ◽  
Binding Activity ◽  
Single Class ◽  
Nuclear Extracts

ABSTRACT An androgen receptor has been identified in the cytosolic and nuclear extracts of the Harderian gland of the frog, Rana esculenta. A single class of high-affinity binding sites was found: Kd = 1·9±1·3 (s.d.) nmol/l (n = 26) for the cytosolic extract and Kd = 0·9±0·8 nmol/l (n = 15) for the nuclear extract. The presence of binding activity in both nuclear and cytosolic extracts and the low rate of ligand-receptor dissociation are characteristics that distinguish this receptor from a steroid-binding protein. The Kd did not show any sex difference and did not exhibit any secretory activity-related change. Binding in both cytosolic and nuclear extracts was specific for androgens (testosterone = 5α-dihydrotestosterone); oestradiol-17β showed a 30% cross-reaction; moreover, specific binding of [3H]oestradiol-17β was not detectable. The binding capacity of the Harderian gland increased progressively in both fractions from October to December, reaching a peak in May, and decreased suddenly during July to August. The lack of any morphological sex-related difference in the Harderian gland of the green frog might be accounted for by the high amount of circulating androgens as well as a similar concentration of androgen receptor in both sexes. Journal of Endocrinology (1991) 129, 227–232

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