Linkage mapping of sbm-1, a gene conferring resistance to pea seed-borne mosaic virus, using molecular markers in Pisum sativum

1993 ◽  
Vol 85 (5) ◽  
pp. 609-615 ◽  
Author(s):  
G. M. Timmerman ◽  
T. J. Frew ◽  
A. L. Miller ◽  
N. F. Weeden ◽  
W. A. Jermyn
Keyword(s):  
Molecular Markers ◽  
Pisum Sativum ◽  
Mosaic Virus ◽  
Linkage Mapping ◽  
Pea Seed

scholarly journals Inheritance of Resistance to a Third Pathotype of Pea Seed-Borne Mosaic Virus in Pisum sativum

1988 ◽  
Vol 79 (1) ◽  
pp. 76-77 ◽  
Author(s):  
R. Provvidenti ◽  
R. Alconero
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Inheritance Of Resistance ◽  
Pea Seed

scholarly journals Inheritance of Resistance to a Lentil Strain of Pea Seed-Borne Mosaic Virus in Pisum sativum

1988 ◽  
Vol 79 (1) ◽  
pp. 45-47 ◽  
Author(s):  
R. Provvidenti ◽  
R. Alconero
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Inheritance Of Resistance ◽  
Pea Seed

SCREENING OF FIELD PEA BREEDING LINES FOR PEA SEED-BORNE MOSAIC VIRUS

1979 ◽  
Vol 59 (1) ◽  
pp. 171-175 ◽  
Author(s):  
S. T. ALI-KHAN ◽  
R. C. ZIMMER
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Field Pea ◽  
Research Station ◽  
Chenopodium Amaranticolor ◽  
Breeding Lines ◽  
Pisum Sativum L ◽  
Local Lesion Host ◽  
Extensive Program ◽  
Pea Seed

Pea seed-borne mosaic virus (PSbMV) was first identified in Canadian field pea (Pisum sativum L.) breeding lines in 1974. Since then, an extensive program has been underway to eradicate this virus from the breeding lines. At the Morden Research Station, nearly 2000 breeding lines were evaluated. The virus was assayed by infectivity tests using the local lesion host Chenopodium amaranticolor, and by a gel immunodiffusion test. PSbMV was detected in 1361 lines. The level of infection within lines varied from 1 to 3%. Due to the restricted extent of the virus in the breeding lines, it was possible to continue the breeding program without a serious loss in germplasm.

Proteomics offers insight to the mechanism behind Pisum sativum L. response to pea seed-borne mosaic virus (PSbMV)

2017 ◽  
Vol 153 ◽  
pp. 78-88 ◽  
Author(s):  
Hana Cerna ◽  
Martin Černý ◽  
Hana Habánová ◽  
Dana Šafářová ◽  
Kifah Abushamsiya ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Pisum Sativum L ◽  
Pea Seed

Comparison of some pea seed-borne mosaic virus isolates and their detection by dot-immunobinding assay

1991 ◽  
Vol 42 (3) ◽  
pp. 441 ◽  
Author(s):  
JS Ligat ◽  
D Cartwright ◽  
JW Randles
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Limit Of Detection ◽  
Germination Rate ◽  
Seed Transmission ◽  
Healthy Plant ◽  
Chenopodium Amaranticolor ◽  
Infected Seed ◽  
The Us ◽  
Pea Seed

Five isolates of pea seed-borne mosaic virus (US, S4, S6, Q and T) were compared by host range and symptomatology on 16 Pisum sativum cultivars and lines, 21 lines of Lathyrus and Lens spp. and several indicator species. All selections of Pisum sativum, except cv. Greenfeast, were susceptible to all isolates, but Greenfeast was susceptible to the US isolate. All isolates except T infected the Lathyrus and Lens spp. through mechanical and aphid transmissions. Chenopodium amaranticolor and Vicia faba reacted similarly to all isolates, Phaseolus vulgaris cv. Hawkesbury Wonder reacted to none. The North American isolate (US) was distinguished from the Australian S4, S6, Q, and T isolates by infecting Nicotiana clevelandii and Greenfeast pea. In all cases the highest rate of seed transmission occurred in the largest seed (82-91%) and the lowest was in the smallest seed (27-40%). Infected seed in the largest size classes was lighter in weight than the corresponding uninfected seed. Infected seed in all classes had a significantly lower germination rate than uninfected seed although the greatest reduction in germinability was in the smallest seed. In each size class uninfected seed was heavier than infected seed and germinated better. Two-dimensional immunodiffusion tests showed that precipitin lines between all the isolates and either the US and S6 antisera were confluent with no evidence of spurs. A rapid and sensitive indirect dot-immunobinding assay on nitrocellulose membrane for PSbMV was developed in which non-specific reactions were eliminated by using mannose and glucose in buffers, and healthy plant sap as a blocking agent. The limit of detection of antigen was about 32 ng per sample. Both of the antisera detected antigen in sap extracted from peas infected with the 6 PSbMV isolates, originating from the USA, Australia, New Zealand and Denmark and all isolates were detected at similar antiserum dilution endpoints.

Serological Methods for Detection of Pea Seed-Borne Mosaic Virus in Leaves and Seeds of Pisum sativum

1978 ◽  
Vol 68 (4) ◽  
pp. 539 ◽  
Author(s):  
R. I. Hamilton
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Pea Seed

scholarly journals Detection of pea seed-borne mosaic virus virion and pinwheel inclusion body proteins in leaf and seed tissues of pea (Pisum sativum L) by immunogold labelling

Agronomie ◽  
1991 ◽  
Vol 11 (5) ◽  
pp. 387-394 ◽  
Author(s):  
D. Wang ◽  
RD Woods ◽  
AG Swaby ◽  
AJ Cockbain
Keyword(s):  
Pisum Sativum ◽  
Mosaic Virus ◽  
Inclusion Body ◽  
Immunogold Labelling ◽  
Pisum Sativum L ◽  
Pea Seed

β-Amyrin Synthase1 Controls the Accumulation of the Major Saponins Present in Pea (Pisum sativum)

2021 ◽  
Author(s):  
Vanessa Vernoud ◽  
Ludivine Lebeigle ◽  
Jocelyn Munier ◽  
Julie Marais ◽  
Myriam Sanchez ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Bitter Taste ◽  
Crop Improvement ◽  
Functional Protein ◽  
Saponin Biosynthesis ◽  
Physiological Quality ◽  
Pea Seeds ◽  
Identification And Characterization ◽  
Pea Seed

Abstract The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes which can impart an undesirable bitter taste when used as an ingredient in foodstuffs. In this paper, we describe the identification and characterization of a gene involved in saponin biosynthesis during pea seed development, by screening mutants obtained from two Pisum sativum TILLING (Targeting Induced Local Lesions in Genomes) populations in two different genetic backgrounds. The mutations studied are located in a gene designated PsBAS1 (β-amyrin synthase1) which is highly expressed in maturing pea seeds and which encodes a protein previously shown to correspond to an active β-amyrin synthase. The first allele is a nonsense mutation, while the second mutation is located in a splice site and gives rise to a mis-spliced transcript encoding a truncated, non-functional protein. The homozygous mutant seeds accumulated virtually no saponin without affecting seed nutritional or physiological quality. Interestingly, BAS1 appears to control saponin accumulation in all other tissues of the plant examined. These lines represent a first step in the development of pea varieties lacking bitterness off-flavours in their seeds. Our work also shows that TILLING populations in different genetic backgrounds represent valuable genetic resources for both crop improvement and functional genomics.

Sign in / Sign up

Export Citation Format

Share Document