Restriction endonuclease digest patterns of chromosomal DNA from nitrate-negative Campylobacter jejuni-like organisms

1985 ◽  
Vol 1 (4) ◽  
Author(s):  
R.J. Owen ◽  
A. Beck ◽  
P. Borman
Keyword(s):  
Restriction Endonuclease ◽  
Campylobacter Jejuni ◽  
Chromosomal Dna ◽  
Restriction Endonuclease Digest

Ribosomal RNA Gene Restriction Fragment Diversity amongst Penner Serotypes of Campylobacter jejuni and Campylobacter coli

1998 ◽  
Vol 53 (1-2) ◽  
pp. 65-68
Author(s):  
S. I. Smith ◽  
D. K. Olukoya ◽  
A. J. Fox ◽  
A. O. Coker
Keyword(s):  
Campylobacter Jejuni ◽  
Ribosomal Rna ◽  
Rrna Gene ◽  
Campylobacter Coli ◽  
Chromosomal Dna ◽  
Ribosomal Rna Gene ◽  
Restriction Endonuclease Digest ◽  
Gene Restriction ◽  
Rna Genes ◽  
The 16S Rrna Gene

AbstractDiversity based on ribosomal RNA gene-restriction endonuclease digest patterns was detected amongst forty-seven strains of Campylobacter made up of 38 strains of Campylobacter jejuni and 9 strains of Campylobacter coli. Restriction digests of chromosomal DNA prepared by treating with Hae III were probed with an oligonucleotide specific for Campylobacter 16S ribosomal RNA genes. Seventeen distinct hybridization patterns, each indicating the presence of 2 - 4 copies of the 16S rRNA gene are encoded in Campylobacter DNA. Differences in fragment patterns were observed not only between members of two species, but also between individual strains of the same species. Ribopattern fragments of 8.71, 7.56, 2.81 and 1.0 kb were characteristic of the majority of C. jejuni, whereas 7.59 and 4.68 kb fragments were commonly present in C. coli.In conclusion, Hae III ribotyping was even more discriminatory than the Penner serotyping of C. jejuni and C. coli, as strains of the same serotype were distinguished.

scholarly journals Restriction endonuclease digest patterns of chromosomal DNA from group B  -haemolytic streptococci

1991 ◽  
Vol 35 (5) ◽  
pp. 297-303 ◽  
Author(s):  
Y. NAGANO ◽  
N. NAGANO ◽  
S. TAKAHASHI ◽  
K. MURONO ◽  
K. FUJITA ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Chromosomal Dna ◽  
Restriction Endonuclease Digest ◽  
Group B

scholarly journals Clonal Distribution of Invasive Neisseria meningitidis Isolates from the Norwegian County of Telemark, 1987 to 1995

1998 ◽  
Vol 36 (9) ◽  
pp. 2623-2628 ◽  
Author(s):  
Randi Kersten Aakre ◽  
Andrew Jenkins ◽  
Bjørn-Erik Kristiansen ◽  
L. Oddvar Frøholm
Keyword(s):  
Neisseria Meningitidis ◽  
Restriction Endonuclease ◽  
Meningococcal Disease ◽  
Restriction Endonuclease Analysis ◽  
Serological Analysis ◽  
Chromosomal Dna ◽  
Phenotypic Characteristics ◽  
Class 1 ◽  
Clonal Distribution ◽  
Endonuclease Analysis

Forty-two Neisseria meningitidis isolates were obtained from patients with meningococcal disease in the Norwegian county of Telemark (January 1987 to March 1995), and all were compared by PCR amplicon restriction endonuclease analysis (PCR-AREA) of thedhps gene, chromosomal DNA fingerprinting, and serological analysis. PCR-AREA divided the isolates into 11 classes, of which 4, comprising 15, 8, 6, and 2 isolates, were clonal while the remaining 8 classes were genetically heterogeneous or contained only 1 isolate. Three of the four clonal classes could be tentatively equated with recognized epidemic clones (ET5, ET37, and cluster A4) on the basis of their phenotypic characteristics, while the remaining clone appears to be new. There were significant differences in the geographical distribution of clones, with class 1 (ET5-like) isolates significantly overrepresented in rural parts of Telemark. Class 1 (ET5-like) isolates occurred throughout the study period and were dominant in 1987. Class 2 (ET37-like) isolates occurred from 1988 to 1992, and class 3 isolates (with no recognizable ET affinities) were found only in 1991 and 1992.

scholarly journals Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan

1989 ◽  
Vol 103 (3) ◽  
pp. 487-496 ◽  
Author(s):  
P. Shears ◽  
G. Suliman ◽  
C. A. Hart
Keyword(s):  
Antimicrobial Resistance ◽  
Restriction Endonuclease ◽  
Restriction Enzymes ◽  
Surveillance Systems ◽  
High Prevalence ◽  
Resistance Patterns ◽  
Reproducible Method ◽  
Restriction Endonuclease Digest

SUMMARYThe investigation of plasmid similarity is an important component in the surveillance of antimicrobial resistance and in the detection of epidemic plasmids. The use of restriction endonucleases in the classification of transferable, multiply-resistant plasmids from faecal Enterobacteriaceae isolated at the Children's Emergency Hospital, Khartoum was investigated. Twenty-four transconjugant plasmids, coding for 11 different resistance patterns, each of molecular weight 62 MDa. were studied using four restriction enzymes;PstI,EcoR I,HindIII andAraII. Fifteen different digest profiles were obtained. Restriction profiles discriminated between plasmids with differing resistance patterns and demonstrated homology of plasmids with common resistance patterns. Restriction endonuclease digest patterns provide a potentially rapid and reproducible method of plasmid classification, that could contribute towards surveillance systems in tropical countries with a high prevalence of antimicrobial resistance.

scholarly journals Restriction enzyme analysis and ribotyping distinguish Bordetella avium and Bordetella hinzii isolates

2000 ◽  
Vol 124 (1) ◽  
pp. 83-90 ◽  
Author(s):  
R. E. SACCO ◽  
K. B. REGISTER ◽  
G. E. NORDHOLM
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Enzyme ◽  
Restriction Endonucleases ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Bacterial Isolates ◽  
Chromosomal Dna ◽  
Bordetella Avium ◽  
Typing System

Fifty-seven bacterial isolates previously identified as Bordetella avium or B. hinzii were characterized by restriction enzyme analysis (REA) and/or ribotyping. Twenty restriction endonucleases were evaluated for REA. Digestion of chromosomal DNA from the 42 B. avium and 15 B. hinzii isolates with Hinf I produced 8 and 7 distinct fingerprint profiles, respectively. Digestion with DdeI further discriminated these Bordetella species and produced 12 fingerprint profiles for B. avium and 4 profiles of B. hinzii. In addition, B. avium isolates were clearly distinguishable from B. hinzii isolates by ribotyping with the restriction endonuclease PvuII. The ribotype patterns of these two species of Bordetella were unique when compared to previously reported ribotype patterns for B. bronchiseptica isolates. Since it was possible to discern differences among isolates within each Bordetella species by REA analysis, we suggest that REA could be used in developing a typing system based on the fingerprint profiles generated.

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