Industrial applications of a cloned neutral protease gene in Bacillus subtilis

1986 ◽  
Vol 24 (4) ◽  
Author(s):  
SharonJ. Reid ◽  
J.Ann Sugrue ◽  
JenniferA. Thomson
Keyword(s):  
Bacillus Subtilis ◽  
Industrial Applications ◽  
Neutral Protease ◽  
Protease Gene

scholarly journals Expression of the Neutral Protease Gene from a Thermophilic Bacillus sp. BT1 Strain in Bacillus subtilis and Its Natural Host: Identification of a Functional Promoter

2000 ◽  
Vol 182 (14) ◽  
pp. 4104-4107 ◽  
Author(s):  
Branislav Večerek ◽  
Gerard Venema
Keyword(s):  
Bacillus Subtilis ◽  
Natural Host ◽  
Neutral Protease ◽  
Protease Gene ◽  
Reading Frame ◽  
Reverse Transcription Pcr ◽  
Gene Open Reading Frame ◽  
Efficient Expression ◽  
Rapid Amplification ◽  
Host Identification

ABSTRACT The expression of the neutral protease gene (npr) from the thermophilic Bacillus sp. BT1 strain was studied in its natural host and in mesophilic Bacillus subtilis. In the thermophilic BT1 strain, the transcription of the protease gene is initiated from its own promoter, just 5′ to the gene. In contrast, in heterologous B. subtilis this thermophilic nprpromoter does not function, and expression of the npr gene results from transcription originating upstream of an adjacent gene, open reading frame X (ORF X). A functional promoter was identified 5′ to ORF X that is required for efficient expression of thenpr gene in Bacillus subtilis as verified by primer extension, reverse transcription-PCR, and 5′ rapid amplification of cDNA ends experiments. These data suggest that transcriptional signals used in thermophilic Bacillus sp. BT1 strain are different from those used in B. subtilis.

Nucleotide sequence of the neutral protease gene from Bacillus subtilis var. amylosacchariticus

1990 ◽  
Vol 70 (6) ◽  
pp. 370-375 ◽  
Author(s):  
Tadashi Yoshimoto ◽  
Hiroshi Oyama ◽  
Tomoaki Takeshita ◽  
Hideaki Higashi ◽  
Sheng-lin Xu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Nucleotide Sequence ◽  
Neutral Protease ◽  
Protease Gene
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