Identification of a genomic region that complements a temperature-sensitive, high CO2-requiring mutant of the cyanobacterium, Synechococcus sp. PCC7942

1991 ◽  
Vol 226 (3) ◽  
pp. 401-408 ◽  
Author(s):  
Eiji Suzuki ◽  
Hideya Fukuzawa ◽  
Shigetoh Miyachi
Keyword(s):  
Genomic Region ◽  
Temperature Sensitive ◽  
High Co2 ◽  
Synechococcus Sp

scholarly journals The Genomic Region of rbcLS in Synechococcus sp. PCC 7942 Contains Genes Involved in the Ability to Grow under Low CO2 Concentration and in Chlorophyll Biosynthesis

1995 ◽  
Vol 108 (4) ◽  
pp. 1461-1469 ◽  
Author(s):  
M. Ronen-Tarazi ◽  
J. Lieman-Hurwitz ◽  
C. Gabay ◽  
M. I. Orus ◽  
A. Kaplan
Keyword(s):  
Chlorophyll Biosynthesis ◽  
Co2 Concentration ◽  
Genomic Region ◽  
Low Co2 ◽  
Synechococcus Sp ◽  
Pcc 7942

scholarly journals Regulation of asparagine synthetase gene expression by amino acid starvation

1991 ◽  
Vol 11 (12) ◽  
pp. 6059-6066
Author(s):  
S S Gong ◽  
L Guerrini ◽  
C Basilico
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Transcription Initiation ◽  
Rna Stability ◽  
Genomic Region ◽  
Amino Acid Starvation ◽  
Asparagine Synthetase ◽  
Temperature Sensitive ◽  
Mrna Levels ◽  
Regulation Of Expression

We have studied the regulation of expression of the asparagine synthetase (AS) gene in ts11 cells, a mutant of BHK hamster cells which encodes a temperature-sensitive AS and therefore does not produce endogenous asparagine at 39.5 degrees C. Incubation of ts11 cells at the nonpermissive temperature drastically increases the level of AS mRNA, and the stimulation of AS mRNA expression is effectively suppressed by the addition of asparagine to the medium. We show here that regulation of AS gene expression involves cis-acting elements which are contained in the mRNA as well as in the 5' genomic region. When a plasmid containing the human AS cDNA under the control of the human AS promoter region was stably transfected into ts11 cells, the expression of human AS RNAs was regulated as that of the endogenous hamster transcripts, indicating that this construct contained all cis elements necessary for regulation. Expression of the AS cDNA in ts11 cells under the control of a constitutive foreign promoter was also regulated by the concentration of asparagine, and this regulation required translation. When we introduced by mutagenesis a number of stop codons in the AS cDNA, the mutant mRNAs with short open reading frames were expressed at low levels that were not increased by asparagine deprivation. Inhibition of protein and RNA synthesis also prevented down-regulation of AS mRNA levels by high concentrations of asparagine. In a parallel series of experiments, we showed that an AS DNA fragment including the promoter and first exon can also regulate RNA expression in response to asparagine concentration. Furthermore, similar increases in the levels of AS RNAs are produced not only by asparagine deprivation in ts11 cells but also by deprivation of human and wild-type BHK cells of leucine, isoleucine, or glutamine. Thus, regulation of AS gene expression is a response to amino acid starvation through mechanisms which appear to involve both changes in RNA stability and change in the rates of transcription initiation or elongation.

Chemical Mutation Method for High CO2-Requiring-Mutants of the Cyanobacterium Synechococcus sp. PCC 7002

2018 ◽  
pp. 53-62
Author(s):  
Ummi Syuhada Halmi Shari ◽  
Azlin Suhaida Azmi ◽  
Azura Amid
Keyword(s):  
High Co2 ◽  
Synechococcus Sp

scholarly journals Phenotypic Complementation of High CO2-Requiring Mutants of the Cyanobacterium Synechococcus sp. Strain PCC 7942 by Inosine 5′-Monophosphate

1992 ◽  
Vol 100 (4) ◽  
pp. 1987-1993 ◽  
Author(s):  
Rakefet Schwarz ◽  
Judy Lieman-Hurwitz ◽  
Miriam Hassidim ◽  
Aaron Kaplan
Keyword(s):  
High Co2 ◽  
Synechococcus Sp ◽  
Pcc 7942

scholarly journals High CO2 Concentration Alleviates the Block in Photosynthetic Electron Transport in an ndhB-Inactivated Mutant of Synechococcus sp. PCC 7942

1993 ◽  
Vol 101 (3) ◽  
pp. 1047-1053 ◽  
Author(s):  
E. Marco ◽  
N. Ohad ◽  
R. Schwarz ◽  
J. Lieman-Hurwitz ◽  
C. Gabay ◽  
...  
Keyword(s):  
Electron Transport ◽  
Photosynthetic Electron Transport ◽  
Co2 Concentration ◽  
High Co2 ◽  
Synechococcus Sp ◽  
High Co2 Concentration ◽  
Pcc 7942

scholarly journals A High Temperature-Sensitive Mutant of Synechococcus sp. PCC 7002 with Modifications in the Endogenous Plasmid, pAQ1

2002 ◽  
Vol 43 (2) ◽  
pp. 217-223 ◽  
Author(s):  
Aiko Kimura ◽  
Tomoko Hamada ◽  
Eugene H. Morita ◽  
Hidenori Hayashi
Keyword(s):  
High Temperature ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Synechococcus Sp

scholarly journals Ribosomal S27a Coding Sequences Upstream of Ubiquitin Coding Sequences in the Genome of a Pestivirus

1998 ◽  
Vol 72 (11) ◽  
pp. 8697-8704 ◽  
Author(s):  
Paul Becher ◽  
Michaela Orlich ◽  
Heinz-Jürgen Thiel
Keyword(s):  
Molecular Cloning ◽  
Genomic Region ◽  
Nucleotide Sequencing ◽  
Temperature Sensitive ◽  
Nonstructural Proteins ◽  
Transient Expression Assay ◽  
Sequence Comparisons ◽  
Coding Sequences ◽  
Diarrhea Virus ◽  
Processing Signal

ABSTRACT Molecular characterization of cytopathogenic (cp) bovine viral diarrhea virus (BVDV) strain CP Rit, a temperature-sensitive strain widely used for vaccination, revealed that the viral genomic RNA is about 15.2 kb long, which is about 2.9 kb longer than the one of noncytopathogenic (noncp) BVDV strains. Molecular cloning and nucleotide sequencing of parts of the genome resulted in the identification of a duplication of the genomic region encoding nonstructural proteins NS3, NS4A, and part of NS4B. In addition, a nonviral sequence was found directly upstream of the second copy of the NS3 gene. The 3′ part of this inserted sequence encodes an N-terminally truncated ubiquitin monomer. This is remarkable since all described cp BVDV strains with ubiquitin coding sequences contain at least one complete ubiquitin monomer. The 5′ region of the nonviral sequence did not show any homology to cellular sequences identified thus far in cp BVDV strains. Databank searches revealed that this second cellular insertion encodes part of ribosomal protein S27a. Further analyses included molecular cloning and nucleotide sequencing of the cellular recombination partner. Sequence comparisons strongly suggest that the S27a and the ubiquitin coding sequences found in the genome of CP Rit were both derived from a bovine mRNA encoding a hybrid protein with the structure NH2-ubiquitin-S27a-COOH. Polyprotein processing in the genomic region encoding the N-terminal part of NS4B, the two cellular insertions, and NS3 was studied by a transient-expression assay. The respective analyses showed that the S27a-derived polypeptide, together with the truncated ubiquitin, served as processing signal to yield NS3, whereas the truncated ubiquitin alone was not capable of mediating the cleavage. Since the expression of NS3 is strictly correlated with the cp phenotype of BVDV, the altered genome organization leading to expression of NS3 most probably represents the genetic basis of cytopathogenicity of CP Rit.

scholarly journals Photosynthetic Pigments Changes of Three Phenotypes of Picocyanobacteria Synechococcus sp. under Different Light and Temperature Conditions

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2030 ◽  
Author(s):  
Sylwia Śliwińska-Wilczewska ◽  
Zofia Konarzewska ◽  
Kinga Wiśniewska ◽  
Marta Konik
Keyword(s):  
Net Primary Production ◽  
Model Development ◽  
Global Ocean ◽  
Temperature Sensitive ◽  
Total Pigment ◽  
Wide Range ◽  
Rate Of Photosynthesis ◽  
Synechococcus Sp

It is estimated that the genus Synechococcus is responsible for about 17% of net primary production in the Global Ocean. Blooms of these organisms are observed in tropical, subtropical and even temperate zones, and they have been recorded recently even beyond the polar circle. The long-term scenarios forecast a growing expansion of Synechococcus sp. and its area of dominance. This is, among others, due to their high physiological plasticity in relation to changing environmental conditions. Three phenotypes of the genus Synechococcus sp. (Type 1, Type 2, and Type 3a) were tested in controlled laboratory conditions in order to identify their response to various irradiance (10, 55, 100 and 145 µmol photons m−2 s−1) and temperature (15, 22.5 and 30 °C) conditions. The highest total pigment content per cell was recorded at 10 μmol photons m−2 s−1 at all temperature variants with the clear dominance of phycobilins among all the pigments. In almost every variant the highest growth rate was recorded for the Type 1. The lowest growth rates were observed, in general, for the Type 3a. However, it was recognized to be less temperature sensitive in comparison to the other two types and rather light-driven with the highest plasticity and adaptation potential. The highest amounts of carotenoids were produced by Type 2 which also showed signs of the cell stress even around 55 μmol photons m−2 s−1 at 15 °C and 22.5 °C. This may imply that the Type 2 is the most susceptible to higher irradiances. Picocyanobacteria Synechococcus sp. require less light intensity to achieve the maximum rate of photosynthesis than larger algae. They also tolerate a wide range of temperatures which combined together make them gain a powerful competitive advantage. Our results will provide key information for the ecohydrodynamical model development. Thus, this work would be an important link in forecasting future changes in the occurrence of these organisms in the context of global warming.

scholarly journals Regulation of asparagine synthetase gene expression by amino acid starvation.

1991 ◽  
Vol 11 (12) ◽  
pp. 6059-6066 ◽  
Author(s):  
S S Gong ◽  
L Guerrini ◽  
C Basilico
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Transcription Initiation ◽  
Rna Stability ◽  
Genomic Region ◽  
Amino Acid Starvation ◽  
Asparagine Synthetase ◽  
Temperature Sensitive ◽  
Mrna Levels ◽  
Regulation Of Expression

We have studied the regulation of expression of the asparagine synthetase (AS) gene in ts11 cells, a mutant of BHK hamster cells which encodes a temperature-sensitive AS and therefore does not produce endogenous asparagine at 39.5 degrees C. Incubation of ts11 cells at the nonpermissive temperature drastically increases the level of AS mRNA, and the stimulation of AS mRNA expression is effectively suppressed by the addition of asparagine to the medium. We show here that regulation of AS gene expression involves cis-acting elements which are contained in the mRNA as well as in the 5' genomic region. When a plasmid containing the human AS cDNA under the control of the human AS promoter region was stably transfected into ts11 cells, the expression of human AS RNAs was regulated as that of the endogenous hamster transcripts, indicating that this construct contained all cis elements necessary for regulation. Expression of the AS cDNA in ts11 cells under the control of a constitutive foreign promoter was also regulated by the concentration of asparagine, and this regulation required translation. When we introduced by mutagenesis a number of stop codons in the AS cDNA, the mutant mRNAs with short open reading frames were expressed at low levels that were not increased by asparagine deprivation. Inhibition of protein and RNA synthesis also prevented down-regulation of AS mRNA levels by high concentrations of asparagine. In a parallel series of experiments, we showed that an AS DNA fragment including the promoter and first exon can also regulate RNA expression in response to asparagine concentration. Furthermore, similar increases in the levels of AS RNAs are produced not only by asparagine deprivation in ts11 cells but also by deprivation of human and wild-type BHK cells of leucine, isoleucine, or glutamine. Thus, regulation of AS gene expression is a response to amino acid starvation through mechanisms which appear to involve both changes in RNA stability and change in the rates of transcription initiation or elongation.

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