Instability of plasmid DNA sequences: Macro and micro evolution of the antibiotic resistance plasmid R6-5

1978 ◽  
Vol 167 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Kenneth N. Timmis ◽  
Felipe Cabello ◽  
Isabel Andrés ◽  
Alfred Nordheim ◽  
Hans J. Burkhardt ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Dna Sequences ◽  
Plasmid Dna ◽  
Resistance Plasmid

Interspecific relationships of antibiotic resistance in Staphylococcus sp.: isolation and comparison of plasmids determining tetracycline resistance in S. aureus and S. epidermidis

1979 ◽  
Vol 25 (12) ◽  
pp. 1468-1475 ◽  
Author(s):  
David J. Groves
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Dna Sequences ◽  
Plasmid Dna ◽  
Tetracycline Resistance ◽  
Cleavage Sites ◽  
Dna Homology ◽  
Reference Plasmid ◽  
High Degree ◽  
Degree Of Similarity

Tetracycline resistance in Staphylococcus aureus and S. epidermidis was confirmed to be determined by plasmids of the same size. Digestion of plasmids from each strain with restriction endonucleases EcoRl, HindIII, and AluI showed a high degree of similarity in their DNA sequences. At least 10 cleavage sites which appear to be common to both plasmids were detected. An additional three cleavage sites appear to be unique to the S. epidermidis plasmid. Further, a survey of recent clinical isolates of tetracycline-resistant staphylococci detected 7 of 10 S. aureus strains and 8 of 9 S. epidermidis strains with plasmids which were of similar size to the purified reference plasmids and which, by hybridization, showed extensive DNA homology to the S. aureus reference plasmid DNA.

Large antibiotic-resistance plasmid of Edwardsiella tarda contributes to virulence in fish

2012 ◽  
Vol 52 (5) ◽  
pp. 259-266 ◽  
Author(s):  
Jong Earn Yu ◽  
Mi Young Cho ◽  
Jin-woo Kim ◽  
Ho Young Kang
Keyword(s):  
Antibiotic Resistance ◽  
Edwardsiella Tarda ◽  
Resistance Plasmid

scholarly journals Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Daouda A. K. Traore ◽  
Jessica A. Wisniewski ◽  
Sarena F. Flanigan ◽  
Paul J. Conroy ◽  
Santosh Panjikar ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Antibiotic Resistance ◽  
Resistance Plasmid

scholarly journals Multiple Displacement Amplification as a Solution for Low Copy Number Plasmid Sequencing

2021 ◽  
Vol 12 ◽  
Author(s):  
Kuan Yao ◽  
Narjol González-Escalona ◽  
Maria Hoffmann
Keyword(s):  
Antibiotic Resistance ◽  
Single Molecule ◽  
Plasmid Dna ◽  
Copy Number ◽  
Sequence Data ◽  
Multiple Displacement Amplification ◽  
Fast Method ◽  
Alkaline Lysis ◽  
Low Copy Number ◽  
Long Read

Plasmids play a major role in bacterial adaptation to environmental stress and often contribute to antibiotic resistance and disease virulence. Although the complete sequence of each plasmid is essential for studying plasmid biology, most antibiotic resistance and virulence plasmids in Salmonella are present only in a low copy number, making extraction and sequencing difficult. Long read sequencing technologies require higher concentrations of DNA to provide optimal results. To resolve this problem, we assessed the sufficiency of multiple displacement amplification (MDA) for replicating Salmonella plasmid DNA to a satisfactory concentration for accurate sequencing and multiplexing. Nine Salmonella enterica isolates, representing nine different serovars carrying plasmids for which sequence data are already available at NCBI, were cultured and their plasmids isolated using an alkaline lysis extraction protocol. We then used the Phi29 polymerase to perform MDA, thereby obtaining enough plasmid DNA for long read sequencing. These amplified plasmids were multiplexed and sequenced on one single molecule, real-time (SMRT) cell with the Pacific Biosciences (Pacbio) Sequel sequencer. We were able to close all Salmonella plasmids (sizes ranged from 38 to 166 Kb) with sequencing coverage from 24 to 2,582X. This protocol, consisting of plasmid isolation, MDA, and multiplex sequencing, is an effective and fast method for closing high-molecular weight and low-copy-number plasmids. This high throughput protocol reduces the time and cost of plasmid closure.

scholarly journals Genetic Diversity of Xanthomonas campestris pv. vitians, the Causal Agent of Bacterial Leafspot of Lettuce

2003 ◽  
Vol 93 (5) ◽  
pp. 596-603 ◽  
Author(s):  
Jeri D. Barak ◽  
Robert L. Gilbertson
Keyword(s):  
Genetic Diversity ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Plasmid Dna ◽  
Xanthomonas Campestris ◽  
Rflp Analysis ◽  
Dna Probe ◽  
Internal Transcribed Spacer Region ◽  
Geographical Regions

Bacterial leafspot of lettuce (BLS), caused by Xanthomonas campes-tris pv. vitians, has become more prevalent in many lettuce-growing areas of the world over the past decade. To gain insight into the nature of these outbreaks, the genetic variation in X. campestris pv. vitians strains from different geographical locations was examined. All strains were first tested for pathogenicity on lettuce plants, and then genetic diversity was assessed using (i) gas-chromatographic analysis of bacterial fatty acids, (ii) polymerase chain reaction analysis of repetitive DNA sequences (rep-PCR), (iii) DNA sequence analysis of the internal transcribed spacer region 1 (ITS1) of the ribosomal RNA, (iv) restriction fragment length polymorphism (RFLP) analysis of total genomic DNA with a repetitive DNA probe, and (v) detection and partial characterization of plasmid DNA. Fatty acid analysis identified all pathogenic strains as X. campestris, but did not consistently identify all the strains as X. campestris pv. vitians. The rep-PCR fingerprints and ITS1 sequences of all pathogenic X. campestris pv. vitians strains examined were identical, and distinct from those of the other X. campestris pathovars. Thus, these characteristics did not reveal genetic diversity among X. campestris pv. vitians strains, but did allow for differentiation of X. campestris pathovars. Genetic diversity among X. campestris pv. vitians strains was revealed by RFLP analysis with a repetitive DNA probe and by characterization of plasmid DNA. This diversity was greatest among strains from different geographical regions, although diversity among strains from the same location also was detected. The results of this study suggest that these X. campestris pv. vitians strains are not clonal, but comprise a relatively homogeneous group.

Transcription of the Transfer Genes traY and traM of the Antibiotic Resistance Plasmid R100-1 Is Linked

Plasmid ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 35-48 ◽  
Author(s):  
David Stockwell ◽  
Vera Lelianova ◽  
Teresa Thompson ◽  
Walter B Dempsey
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Plasmid ◽  
Transfer Genes

scholarly journals Real-Time Study of Rapid Spread of Antibiotic Resistance Plasmid in Biofilm Using Microfluidics

2018 ◽  
Vol 52 (19) ◽  
pp. 11132-11141 ◽  
Author(s):  
Bing Li ◽  
Yong Qiu ◽  
Jing Zhang ◽  
Xia Huang ◽  
Hanchang Shi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Real Time ◽  
Time Study ◽  
Resistance Plasmid ◽  
Rapid Spread

POLYPEPTIDES EXPRESSED BY PLASMID DNA SEQUENCES MEDIATING TRIMETHOPRIM RESISTANCE

1980 ◽  
pp. 117-123
Author(s):  
Mary Fling ◽  
Leslie Walton ◽  
Lynn P. Elwell
Keyword(s):  
Dna Sequences ◽  
Plasmid Dna
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