Large antibiotic-resistance plasmid of Edwardsiella tarda contributes to virulence in fish

2012 ◽  
Vol 52 (5) ◽  
pp. 259-266 ◽  
Author(s):  
Jong Earn Yu ◽  
Mi Young Cho ◽  
Jin-woo Kim ◽  
Ho Young Kang
Keyword(s):  
Antibiotic Resistance ◽  
Edwardsiella Tarda ◽  
Resistance Plasmid

scholarly journals Identification and antibiotic resistance profiling of bacterial isolates from septicaemic soft-shelled turtles (Pelodiscus sinensis)

2017 ◽  
Vol 62 (No. 3) ◽  
pp. 169-177 ◽  
Author(s):  
TH Chung ◽  
SW Yi ◽  
BS Kim ◽  
WI Kim ◽  
GW Shin
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Present Report ◽  
Antibiotic Resistance Genes ◽  
Tetracycline Resistance ◽  
Edwardsiella Tarda ◽  
Pelodiscus Sinensis ◽  
Class 1 Integron ◽  
Class 1 ◽  
M 14

The present study sought to identify pathogens associated with septicaemia in the Chinese soft-shelled turtle (Pelodiscus sinensis) and to characterise antibiotic resistance in these pathogens. Twenty-three isolates recovered from the livers of diseased soft-shelled turtles were genetically identified as Aeromonas hydrophila (n = 8), A. veronii (n = 3), Citrobacter freundii (n = 4), Morganella morganii (n = 3), Edwardsiella tarda (n = 2), Wohlfahrtiimonas chitiniclastica (n = 1), Chryseobacterium sp. (n = 1), and Comamonas sp. (n = 1). Most isolates (n = 21) were resistant to ampicillin whereas a low percentage of isolates was susceptible to aminoglycosides (amikacin, gentamicin, and tobramycin). PCR assays and sequence analysis revealed the presence of the qnrS2 and bla<sub>TEM</sub> antibiotic resistance genes in all isolates. The bla<sub>DHA-1</sub>, bla<sub>CTX-M-14</sub> and bla<sub>CMY-2</sub> genes were harboured by 17.4% (n = 4), 13.5% (n = 3) and 8.7% (n = 2) of the strains, respectively. One or more tetracycline resistance genes were detected in 60.9% (n = 14) of the isolates. Four isolates (17.4%) harboured single or multiple class 1 integron cassettes. Collectively, a variety of bacterial pathogens were involved in the occurrence of septicaemia in Chinese soft-shelled turtles and most of the isolates had multi-antibiotic resistant phenotypes. To our knowledge, the present report is the first to identify W. chitiniclastica and Comamonas sp. as causes of septicaemia in soft-shelled turtles and the first to identify Aeromonas spp. with bla<sub>CTX-M-14</sub> and bla<sub>DHA-1</sub> resistance genes.

scholarly journals Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Daouda A. K. Traore ◽  
Jessica A. Wisniewski ◽  
Sarena F. Flanigan ◽  
Paul J. Conroy ◽  
Santosh Panjikar ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Antibiotic Resistance ◽  
Resistance Plasmid

Transcription of the Transfer Genes traY and traM of the Antibiotic Resistance Plasmid R100-1 Is Linked

Plasmid ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 35-48 ◽  
Author(s):  
David Stockwell ◽  
Vera Lelianova ◽  
Teresa Thompson ◽  
Walter B Dempsey
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Plasmid ◽  
Transfer Genes

scholarly journals Real-Time Study of Rapid Spread of Antibiotic Resistance Plasmid in Biofilm Using Microfluidics

2018 ◽  
Vol 52 (19) ◽  
pp. 11132-11141 ◽  
Author(s):  
Bing Li ◽  
Yong Qiu ◽  
Jing Zhang ◽  
Xia Huang ◽  
Hanchang Shi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Real Time ◽  
Time Study ◽  
Resistance Plasmid ◽  
Rapid Spread

scholarly journals Necrotic Enteritis-Derived Clostridium perfringens Strain with Three Closely Related Independently Conjugative Toxin and Antibiotic Resistance Plasmids

mBio ◽  
2011 ◽  
Vol 2 (5) ◽  
Author(s):  
Trudi L. Bannam ◽  
Xu-Xia Yan ◽  
Paul F. Harrison ◽  
Torsten Seemann ◽  
Anthony L. Keyburn ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Clostridium Perfringens ◽  
Tetracycline Resistance ◽  
Necrotic Enteritis ◽  
Sequencing Analysis ◽  
Content Type ◽  
Conjugative Plasmids ◽  
Resistance Plasmid ◽  
Large Plasmids ◽  
Beta2 Toxin

ABSTRACTThe pathogenesis of avian necrotic enteritis involves NetB, a pore-forming toxin produced by virulent avian isolates ofClostridium perfringenstype A. To determine the location and mobility of thenetBstructural gene, we examined a derivative of the tetracycline-resistant necrotic enteritis strain EHE-NE18, in whichnetBwas insertionally inactivated by the chloramphenicol and thiamphenicol resistance genecatP. Both tetracycline and thiamphenicol resistance could be transferred either together or separately to a recipient strain in plate matings. The separate transconjugants could act as donors in subsequent matings, which demonstrated that the tetracycline resistance determinant and thenetBgene were present on different conjugative elements. Large plasmids were isolated from the transconjugants and analyzed by high-throughput sequencing. Analysis of the resultant data indicated that there were actually three large conjugative plasmids present in the original strain, each with its own toxin or antibiotic resistance locus. Each plasmid contained a highly conserved 40-kb region that included plasmid replication and transfer regions that were closely related to the 47-kb conjugative tetracycline resistance plasmid pCW3 fromC. perfringens. The plasmids were as follows: (i) a conjugative 49-kb tetracycline resistance plasmid that was very similar to pCW3, (ii) a conjugative 82-kb plasmid that contained thenetBgene and other potential virulence genes, and (iii) a 70-kb plasmid that carried thecpb2gene, which encodes a different pore-forming toxin, beta2 toxin.IMPORTANCEThe anaerobic bacteriumClostridium perfringenscan cause an avian gastrointestinal disease known as necrotic enteritis. Disease pathogenesis is not well understood, although the plasmid-encoded pore-forming toxin NetB, is an important virulence factor. In this work, we have shown that the plasmid that carries thenetBgene is conjugative and has a 40-kb region that is very similar to replication and transfer regions found within each of the sequenced conjugative plasmids fromC. perfringens. We also showed that this strain contained two additional large plasmids that were also conjugative and carried a similar 40-kb region. One of these plasmids encoded beta2 toxin, and the other encoded tetracycline resistance. To our knowledge, this is the first report of a bacterial strain that carries three closely related but different independently conjugative plasmids. These results have significant implications for our understanding of the transmission of virulence and antibiotic resistance genes in pathogenic bacteria.

Plasmid-encoded Functions Involved in the Replication and Inheritance of Antibiotic-resistance Plasmid R6-5

1979 ◽  
Vol 43 (0) ◽  
pp. 105-110 ◽  
Author(s):  
K. N. Timmis ◽  
I. Andres ◽  
P. M. Slocombe ◽  
R. M. Synenki
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Plasmid

scholarly journals Molecular Analysis of an Antibiotic Resistance Plasmid, pAV5, and Its Derivative Plasmids in Acinetobacter calcoaceticus

Microbiology ◽  
1985 ◽  
Vol 131 (12) ◽  
pp. 3367-3374
Author(s):  
M. DIVERS ◽  
P. L. CRAVEN ◽  
A. VIVIAN
Keyword(s):  
Antibiotic Resistance ◽  
Molecular Analysis ◽  
Acinetobacter Calcoaceticus ◽  
Resistance Plasmid

Effect of growth rate and selection pressure on rates of transfer of an antibiotic resistance plasmid between E. coli strains

Plasmid ◽  
2014 ◽  
Vol 72 ◽  
pp. 1-8 ◽  
Author(s):  
Jasper M. Schuurmans ◽  
Sacha A.F.T. van Hijum ◽  
Jurgen R. Piet ◽  
Nadine Händel ◽  
Jan Smelt ◽  
...  
Keyword(s):  
Growth Rate ◽  
Antibiotic Resistance ◽  
Selection Pressure ◽  
E Coli ◽  
Resistance Plasmid

tra cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5

1978 ◽  
Vol 163 (2) ◽  
pp. 169-179 ◽  
Author(s):  
Mark Achtman ◽  
Barica Kušećek ◽  
Kenneth N. Timmis
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistance Plasmid

scholarly journals Plasmid pAMS1-Encoded, Bacteriocin-Related “Siblicide” in Enterococcus faecalis

2009 ◽  
Vol 191 (9) ◽  
pp. 3183-3188 ◽  
Author(s):  
Christine M. Sedgley ◽  
Don B. Clewell ◽  
Susan E. Flannagan
Keyword(s):  
Antibiotic Resistance ◽  
Sex Pheromone ◽  
Enterococcus Faecalis ◽  
Solid Medium ◽  
Multiple Antibiotic Resistance ◽  
Killing Activity ◽  
Resistance Plasmid ◽  
Zones Of Inhibition

ABSTRACT The Enterococcus faecalis class IIa bacteriocin MC4-1 encoded by the sex pheromone-responding, multiple-antibiotic resistance plasmid pAMS1 exhibits “siblicidal” (sibling-killing) activity under certain conditions. Stabs of plasmid-containing cells on solid medium containing lawns of bacteria of the same (plasmid-containing) strain give rise to zones of inhibition. If the plasmid-containing host also produces gelatinase, bacteriocin cannot be detected.

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