Effects of monomeric acrylic embedding media on the antigenicity of two epitopes of the MIC2-encoded Ewing's sarcoma cell membrane antigen

1992 ◽  
Vol 97 (1) ◽  
pp. 87-94 ◽  
Author(s):  
G. Hamilton ◽  
B. Hamilton ◽  
R. Mallinger
Keyword(s):  
Cell Membrane ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Sarcoma Cell ◽  
Cell Membrane Antigen

scholarly journals An Innovative Therapeutic Option for the Treatment of Skeletal Sarcomas: Elimination of Osteo- and Ewing’s Sarcoma Cells Using Physical Gas Plasma

2020 ◽  
Vol 21 (12) ◽  
pp. 4460 ◽  
Author(s):  
Josephine M. Jacoby ◽  
Silas Strakeljahn ◽  
Andreas Nitsch ◽  
Sander Bekeschus ◽  
Peter Hinz ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cell Lines ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Therapeutic Option ◽  
Bone Sarcoma ◽  
Atmospheric Plasma ◽  
Local Surgery ◽  
Release Assay ◽  
Sarcoma Cells

Osteosarcoma and Ewing’s sarcoma are the most common malignant bone tumors. Conventional therapies such as polychemotherapy, local surgery, and radiotherapy improve the clinical outcome for patients. However, they are accompanied by acute and chronic side effects that affect the quality of life of patients, motivating novel research lines on therapeutic options for the treatment of sarcomas. Previous experimental work with physical plasma operated at body temperature (cold atmospheric plasma, CAP) demonstrated anti-oncogenic effects on different cancer cell types. This study investigated the anti-cancer effect of CAP on two bone sarcoma entities, osteosarcoma and Ewing’s sarcoma, which were represented by four cell lines (U2-OS, MNNG/HOS, A673, and RD-ES). A time-dependent anti-proliferative effect of CAP on all cell lines was observed. CAP-induced alterations in cell membrane functionality were detected by performing a fluorescein diacetate (FDA) release assay and an ATP release assay. Additionally, modifications of the cell membrane and modifications in the actin cytoskeleton composition were examined using fluorescence microscopy monitoring dextran-uptake assay and G-/F-actin distribution. Furthermore, the CAP-induced induction of apoptosis was determined by TUNEL and active caspases assays. The observations suggest that a single CAP treatment of bone sarcoma cells may have significant anti-oncogenic effects and thus may be a promising extension to existing applications.

scholarly journals Inhibition of heat shock proteins (HSP) expression by quercetin and differential doxorubicin sensitization in neuroblastoma and Ewing’s sarcoma cell lines

2007 ◽  
Vol 103 (4) ◽  
pp. 1344-1354 ◽  
Author(s):  
Cristina Zanini ◽  
Giuliana Giribaldi ◽  
Giorgia Mandili ◽  
Franco Carta ◽  
Nicoletta Crescenzio ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Cell Lines ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Sarcoma Cell ◽  
Shock Proteins

scholarly journals Discovery and Validation of a Compound to Target Ewing’s Sarcoma

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1553
Author(s):  
Ellie Esfandiari Nazzaro ◽  
Fahad Y. Sabei ◽  
Walter K. Vogel ◽  
Mohamad Nazari ◽  
Katelyn S. Nicholson ◽  
...  
Keyword(s):  
Cell Lines ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Apoptotic Cell ◽  
Intensive Therapy ◽  
Xenograft Model ◽  
Chemotherapeutic Agents ◽  
Cytotoxic Agents ◽  
Sarcoma Cell

Ewing’s sarcoma, characterized by pathognomonic t (11; 22) (q24; q12) and related chromosomal ETS family translocations, is a rare aggressive cancer of bone and soft tissue. Current protocols that include cytotoxic chemotherapeutic agents effectively treat localized disease; however, these aggressive therapies may result in treatment-related morbidities including second-site cancers in survivors. Moreover, the five-year survival rate in patients with relapsed, recurrent, or metastatic disease is less than 30%, despite intensive therapy with these cytotoxic agents. By using high-throughput phenotypic screening of small molecule libraries, we identified a previously uncharacterized compound (ML111) that inhibited in vitro proliferation of six established Ewing’s sarcoma cell lines with nanomolar potency. Proteomic studies show that ML111 treatment induced prometaphase arrest followed by rapid caspase-dependent apoptotic cell death in Ewing’s sarcoma cell lines. ML111, delivered via methoxypoly(ethylene glycol)-polycaprolactone copolymer nanoparticles, induced dose-dependent inhibition of Ewing’s sarcoma tumor growth in a murine xenograft model and invoked prometaphase arrest in vivo, consistent with in vitro data. These results suggest that ML111 represents a promising new drug lead for further preclinical studies and is a potential clinical development for the treatment of Ewing’s sarcoma.

Abstract A30: Integrative genomic analysis of two Ewing's sarcoma cell lines that show differential drug sensitivity

2014 ◽  
Author(s):  
TABREZ A. MOHAMMAD ◽  
Aparna Gorthi ◽  
Andrew Robles ◽  
Xavier Bernard ◽  
Tzu-hung Hsiao ◽  
...  
Keyword(s):  
Cell Lines ◽  
Drug Sensitivity ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Genomic Analysis ◽  
Sarcoma Cell

scholarly journals Fas-Mediated Apoptosis in Ewing's Sarcoma Cell Lines by Metalloproteinase Inhibitors

1999 ◽  
Vol 91 (19) ◽  
pp. 1678-1684 ◽  
Author(s):  
N. Mitsiades ◽  
V. Poulaki ◽  
A. Leone ◽  
M. Tsokos
Keyword(s):  
Cell Lines ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Sarcoma Cell ◽  
Metalloproteinase Inhibitors

Expression of functional Y1 receptors for neuropeptide Y in human Ewing's sarcoma cell lines

1992 ◽  
Vol 118 (7) ◽  
pp. 529-536 ◽  
Author(s):  
F. van Valen ◽  
W. Winkelmann ◽  
H. Jürgens
Keyword(s):  
Neuropeptide Y ◽  
Cell Lines ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Sarcoma Cell ◽  
Y1 Receptors

EWS/FLI-l peptide-pulsed dendritic cells induces the antitumor immunity in a murine Ewing's sarcoma cell model

2014 ◽  
Vol 21 (2) ◽  
pp. 336-341 ◽  
Author(s):  
Wei Peng ◽  
Xunwu Huang ◽  
Dazhi Yang
Keyword(s):  
Dendritic Cells ◽  
Antitumor Immunity ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Cell Model ◽  
Sarcoma Cell

scholarly journals Overexpression of miR-199b-5p inhibits Ewing's sarcoma cell lines by targeting CCNL1

2015 ◽  
Vol 12 (3) ◽  
pp. 3359-3364 ◽  
Author(s):  
WEIHUA LI ◽  
YUXIA LI ◽  
JIANKUO GUO ◽  
HUAGANG PAN ◽  
YONGLE ZHANG ◽  
...  
Keyword(s):  
Cell Lines ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Sarcoma Cell

Establishment and characterization of a new Ewing's sarcoma cell line

1991 ◽  
Vol 57 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Ken Kodama ◽  
Osamu Doi ◽  
Masahiko Higashiyama ◽  
Yoichi Mori ◽  
Takeshi Horai ◽  
...  
Keyword(s):  
Cell Line ◽  
Ewing’S Sarcoma ◽  
Ewing's Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line
Sign in / Sign up

Export Citation Format

Share Document