The purpose of this study was to examine the reliability of repeated isokinetic knee extension and flexion in young children and to examine sex differences in 30 untrained subjects (16 boys and 14 girls) aged 12.2 ± 0.3 years. Total work and the percentage decline in average torque and work were recorded during 50 repetitions. Intra-class correlation coefficients indicated a strong positive correlation between test 1 and 2 for all parameters ranging from 0.36–0.95. Coefficient of variation data ranged from ± 0% to ± 5.4%. Repeatability coefficients and limits of agreement indicated that the extensors were more reliable than the flexors for both torque and work. There were no significant sex differences in any of the parameters measured. This study suggests that repeated isokinetic muscle actions of the knee, as a function of muscle endurance, can be reliably assessed in young people.