Synthetic substrates in the histochemical demonstration of intestinal disaccharidases

Histochemie ◽  
1973 ◽  
Vol 34 (4) ◽  
pp. 361-369 ◽  
Author(s):  
Zdeněk Lojda ◽  
Jan Slabý ◽  
Jiří Kraml ◽  
Jiřina Kolínská
Keyword(s):  
Histochemical Demonstration ◽  
Intestinal Disaccharidases

The diagnosis of metabolic storage disease in skin biopsies (a light-, electronmicroscopic, and analytical study)

1978 ◽  
Vol 36 (2) ◽  
pp. 648-649
Author(s):  
W. Jurecka ◽  
W. Gebhart ◽  
H. Lassmann
Keyword(s):  
Storage Disease ◽  
Hunter Syndrome ◽  
Histochemical Demonstration ◽  
Sweat Glands ◽  
Type I ◽  
Storage Material ◽  
Scheie Syndrome ◽  
Storage Products ◽  
Skin Biopsies ◽  
Type V

Diagnosis of metabolic storage disease can be established by the determination of enzymes or storage material in blood, urine, or several tissues or by clinical parameters. Identification of the accumulated storage products is possible by biochemical analysis of isolated material, by histochemical demonstration in sections, or by ultrastructural demonstration of typical inclusion bodies. In order to determine the significance of such inclusions in human skin biopsies several types of metabolic storage disease were investigated. The following results were obtained.In MPS type I (Pfaundler-Hurler-Syndrome), type II (Hunter-Syndrome), and type V (Ullrich-Scheie-Syndrome) mainly “empty” vacuoles were found in skin fibroblasts, in Schwann cells, keratinocytes and macrophages (Dorfmann and Matalon 1972). In addition, prominent vacuolisation was found in eccrine sweat glands. The storage material could be preserved in part by fixation with cetylpyridiniumchloride and was also present within fibroblasts grown in tissue culture.

Mitochondrial Oxidation of p-N, N-Dimethylamino-β-Phenethylamine (DAPA)

1975 ◽  
Vol 33 ◽  
pp. 458-459
Author(s):  
W. Allen Shannon ◽  
Hannah L. Wasserkrug ◽  
andArnold M. Seligman
Keyword(s):  
Guinea Pig ◽  
Oxidase Activity ◽  
Amine Oxidase ◽  
Histochemical Demonstration ◽  
Guinea Pig Heart ◽  
Pig Kidney ◽  
Cytoplasmic Vacuoles ◽  
Liver And Kidney ◽  
Mitochondrial Oxidation ◽  
Amine Oxidase Activity

The synthesis of a new substrate, p-N,N-dimethylamino-β-phenethylamine (DAPA)3 (Fig. 1) (1,2), and the testing of it as a possible substrate for tissue amine oxidase activity have resulted in the ultracytochemical localization of enzyme oxidase activity referred to as DAPA oxidase (DAPAO). DAPA was designed with the goal of providing an amine that would yield on oxidation a stronger reducing aldehyde than does tryptamine in the histochemical demonstration of monoamine oxidase (MAO) with tetrazolium salts.Ultracytochemical preparations of guinea pig heart, liver and kidney and rat heart and liver were studied. Guinea pig kidney, known to exhibit high levels of MAO, appeared the most reactive of the tissues studied. DAPAO reaction product appears primarily in mitochondrial outer compartments and cristae (Figs. 2-4). Reaction product is also localized in endoplasmic reticulum, cytoplasmic vacuoles and nuclear envelopes (Figs. 2 and 3) and in the sarcoplasmic reticulum of heart.

scholarly journals Blood Group Antigenicity of Purified Human Intestinal Disaccharidases

1973 ◽  
Vol 248 (23) ◽  
pp. 8216-8221
Author(s):  
John J. Kelly ◽  
David H. Alpers
Keyword(s):  
Blood Group ◽  
Intestinal Disaccharidases

Formation of a Labile Pigment in Rabbit Ova During Histochemical Demonstration of Succinic Dehydrogenase

Science ◽  
1953 ◽  
Vol 117 (3043) ◽  
pp. 449-451 ◽  
Author(s):  
A. G. Foraker ◽  
S. W. Denham ◽  
D. D. Mitchell
Keyword(s):  
Succinic Dehydrogenase ◽  
Histochemical Demonstration

scholarly journals A MODIFIED METHOD OF THE PRUSSIAN BLUE REACTION FOR THE HISTOCHEMICAL DEMONSTRATION OF IRON, AND ITS APPLICATION TO THE COLLOIDAL IRON REACTION OF ACID MUCOPOLYSACCHARIDES

1970 ◽  
Vol 3 (1) ◽  
pp. 18-27 ◽  
Author(s):  
SHUNTA HIROSE ◽  
MASAYUKI YASUTOMI ◽  
NOBUHIRO MURAI ◽  
RYOTARO YAMADA ◽  
ATSUSHI KATAYAMA ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Histochemical Demonstration ◽  
Colloidal Iron ◽  
Modified Method ◽  
Acid Mucopolysaccharides ◽  
Blue Reaction

A histochemical demonstration of γ-aminobutyric acid metabolism in spinal cord

1964 ◽  
Vol 13 (12) ◽  
pp. 1686-1687 ◽  
Author(s):  
Nico M. van Gelder
Keyword(s):  
Spinal Cord ◽  
Acid Metabolism ◽  
Histochemical Demonstration ◽  
Aminobutyric Acid

Histochemical demonstration of monoaminergic and cholinesterase-positive nerve fibres in regenerating rat submandibular gland autografts

1985 ◽  
Vol 17 (6) ◽  
pp. 665-674 ◽  
Author(s):  
Norris L. O'Dell ◽  
Mohamed Sharawy ◽  
Jacob S. Hanker
Keyword(s):  
Submandibular Gland ◽  
Histochemical Demonstration ◽  
Nerve Fibres ◽  
Rat Submandibular Gland

Histochemical demonstration of enzymes in rat liver during post-natal development

1970 ◽  
Vol 23 (1) ◽  
pp. 71-81 ◽  
Author(s):  
J. Koudstaal ◽  
M. J. Hardonk
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration
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