A MODIFIED METHOD OF THE PRUSSIAN BLUE REACTION FOR THE HISTOCHEMICAL DEMONSTRATION OF IRON, AND ITS APPLICATION TO THE COLLOIDAL IRON REACTION OF ACID MUCOPOLYSACCHARIDES

Colloidal iron or ferric chloride injected into the inflamed peritoneal cavity is fixed in the cavity and fails to reach the retrosternal lymphatic nodes, whereas, in the absence of inflammation, iron accumulates in these nodes and becomes demonstrable by the Prussian blue reaction. Quantitative studies show that after intraperitoneal injection of ferric chloride the retrosternal lymphatic nodes of animals with normal peritoneal cavity contain approximately 56 per cent more iron than do the nodes of animals with inflamed peritoneal cavity. Ferric chloride injected into the circulating blood enters an inflamed area in the skin and the inflamed tissue gives the Prussian blue reaction. Quantitative determinations show that the amount of iron in inflamed areas is much greater than that found in inflamed areas of animals that have received no iron.

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THE ACCUMULATION OF IRON IN TUBERCULOUS AREAS

Journal of Experimental Medicine ◽

10.1084/jem.53.6.919 ◽

1931 ◽

Vol 53 (6) ◽

pp. 919-927 ◽

Cited By ~ 16

Author(s):

Valy Menkin ◽

Miriam F. Menkin

Keyword(s):

Prussian Blue ◽

Ferric Chloride ◽

Lung Tissue ◽

Iron Content ◽

Chloride Solution ◽

Ferric Chloride Solution ◽

Intravenous Injections ◽

Blue Reaction

Repeated daily intravenous injections of ferric chloride solution are followed by an accumulation of iron in tuberculous areas of lungs. The iron accumulates in the caseous areas of tubercles and is demonstrable by the Prussian blue reaction. Quantitative determinations corroborate these results and show that the iron content of lung tissue in tuberculous animals injected with ferric chloride exceeds that in normal animals injected with this salt, as well as that in non-injected tuberculous animals.

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Spectrophotometric determination of ascorbic acid in grapes with the Prussian Blue reaction

Analele Universitatii Ovidius Constanta - Seria Chimie ◽

10.2478/v10310-012-0029-y ◽

2012 ◽

Vol 23 (2) ◽

pp. 174-179 ◽

Cited By ~ 2

Author(s):

Nicoleta Matei ◽

Simona Dobrinas ◽

Gabriel Lucian Radu

Keyword(s):

Ascorbic Acid ◽

Vitamin C ◽

Vitis Vinifera ◽

Prussian Blue ◽

Spectrophotometric Determination ◽

Pinot Noir ◽

Cabernet Sauvignon ◽

Red Grapes ◽

Blue Reaction

AbstractThe objective of the present work was to adapt the Prussian Blue reaction for the determination of ascorbic acid. The procedure was successfully applied for the determination of ascorbic acid in red and white grapes (Vitis vinifera L.) just previous ingathering. In the present work was used the red and white grapes from Murfatlar vineyard: Mamaia, Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, Sauvignon, Muscat Ottonel and Riesling Italian. The results were situated in the range of 0.67 - 1.79 mg vitamin C/100g product for red grapes and respectively 0.50 - 1.49 mg vitamin C/100g for white grapes.

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The Application of the Prussian Blue Stain to Previously Stained Films of Blood and Bone Marrow

Blood ◽

10.1182/blood.v10.2.160.160 ◽

1955 ◽

Vol 10 (2) ◽

pp. 160-166 ◽

Cited By ~ 23

Author(s):

R. DOROTHY SUNDBERG ◽

HARRIETTE BROMAN

Keyword(s):

Bone Marrow ◽

Prussian Blue ◽

Formalin Fixation ◽

Soluble Form ◽

Simple Method ◽

Green Material ◽

Green Color ◽

Particulate Iron ◽

Blue Stain ◽

Blue Reaction

Abstract A simple method for staining non-hemoglobin iron in erythrocytes, normoblasts, macrophages, and other cells containing particulate iron in new or old films of cellular fluids or imprints of tissues and organs is presented. This method consists in using the prussian blue reagent as a type of counterstain; no separate decolorization is necessary. The preparations obtained resemble the original preparations except that iron stands out as a vivid blue-green material. The method is particularly useful in studying conditions accompanied by varying degrees of iron excess or hemosiderosis of the marrow. The stainable iron is all virtually the same color. The diffuse blue-green color of the cytoplasm of macrophages might be attributed to the more soluble form of iron, ferritin. Stainable iron is visible in normoblasts and erythrocytes as well as in macrophages in sections subjected to the prussian blue reaction. A prussian blue method using formalin fixation on fresh films of marrow7 has also been shown to be useful in the demonstration of particulate iron in previously stained films of marrow and blood. The formalin fixation appears to bring about a higher percentage of siderocytes.

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THE EFFECTIVENESS OF OUR COLLOIDAL IRON METHOD IN THE ELECTRON-HISTOCHEMICAL DEMONSTRATION OF SULFHYDRYL AND DISULPHIDE WITH PARTICULAR REFERENCE TO PANCREATIC ZYMOGEN GRANULES

ACTA HISTOCHEMICA ET CYTOCHEMICA ◽

10.1267/ahc.8.372 ◽

1975 ◽

Vol 8 (4) ◽

pp. 372-379

Author(s):

NOBUO IHARA ◽

RYUICHI SANO ◽

JUNKO TOKI ◽

RYUEI MAEDA

Keyword(s):

Histochemical Demonstration ◽

Zymogen Granules ◽

Colloidal Iron

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379 Biochemical Barriers Induced by UV Light and Botrytis cinerea in Postharvest Tomato

HortScience ◽

10.21273/hortsci.34.3.509c ◽

1999 ◽

Vol 34 (3) ◽

pp. 509C-509

Author(s):

Marie-Therese Charles ◽

Alain Goulet ◽

Francois Castaigne ◽

Joseph Arul

Keyword(s):

Cell Wall ◽

Disease Resistance ◽

Phenolic Compounds ◽

Prussian Blue ◽

Ultraviolet Light ◽

Uv Light ◽

Tomato Fruit ◽

Induced Disease Resistance ◽

Cell Layers ◽

Blue Reaction

Hormic dose of ultraviolet light (3.7 kJ•m-2) induced disease resistance in tomato fruit. The biochemical nature of induced resistance by UV light was investigated by histochemical techniques. Ultraviolet light induced plasmolysis of the epicarp and few mesocarp cell layers, and collapse of these cell layers led to the formation of cell wall stacking zone (CWSZ). The treatment also stimulated the biosynthesis of phenolic compounds (Prussian blue reaction) in the epicarp and mesocarp cells. Biochemical reinforcement of the cell wall through lignification (Maule test) and suberization (berberine fluorescence) was also induced. These responses originating from the activation of phenylpropanoid path were principally localized in the CWSZ and were induced before inoculation by B. cinerea. The intensity of these responses was significantly increased in UV-treated tissue in response to infection. These responses were also induced in the inoculated control tissue but were either less substantial (phenolics, lignification, and suberization) or delayed.

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