Life cycle variation and regulation of macronuclear DNA content in Tetrahymena thermophila

Chromosoma ◽  
1978 ◽  
Vol 69 (1) ◽  
pp. 1-19 ◽  
Author(s):  
F. P. Doerder ◽  
L. E. DeBault
Keyword(s):  
Life Cycle ◽  
Dna Content ◽  
Tetrahymena Thermophila ◽  
Cycle Variation

Age-associated change in macronuclear DNA content in Paramecium caudatum

1982 ◽  
Vol 54 (1) ◽  
pp. 137-147
Author(s):  
Y. Takagi ◽  
N. Kanazawa
Keyword(s):  
Life Cycle ◽  
Life Span ◽  
Dna Content ◽  
Tetrahymena Thermophila ◽  
Relative Volume ◽  
Paramecium Caudatum ◽  
Unequal Distribution ◽  
Equal Distribution ◽  
Daughter Cells ◽  
Initial Content

Macronuclear DNA content in Paramecium caudatum was found to be almost unchanged with a mean of about 400C during the earlier two-thirds of the life span in terms of the number of fissions and then it dropped rapidly to about one-fifth of the initial content. The age when rapid DNA decline occurred corresponded to that when the characteristics of senescence appeared. This decreasing pattern of macronuclear DNA content contrasted with earlier observations in P. tetraurelia, P. bursaria and Tetrahymena thermophila. The data suggested that in P. caudatum the distribution pattern of macronuclear DNA to daughter cells changed from an almost equal distribution in younger cells to an unequal distribution in older cells, while the relative volume of the macronucleus to the whole cell remained almost constant throughout the life cycle.

Multimodal Life-Cycle Variation in 13- and 17-Year Periodical Cicadas (Hemiptera: Cicadidae: Magicicada)

2017 ◽  
Vol 90 (3) ◽  
pp. 211-226 ◽  
Author(s):  
David C. Marshall ◽  
Kathy B. R. Hill ◽  
John R. Cooley
Keyword(s):  
Life Cycle ◽  
Periodical Cicadas ◽  
Cycle Variation

scholarly journals Life-Cycle Variation in Geophytes

1981 ◽  
Vol 68 (4) ◽  
pp. 652 ◽  
Author(s):  
Amots Dafni ◽  
Dan Cohen ◽  
Imanuel Noy-Mier
Keyword(s):  
Life Cycle ◽  
Cycle Variation

scholarly journals Changes in Plastid DNA Content during the Life Cycle of the Hornwort Anthoceros punctatus L.

CYTOLOGIA ◽  
1999 ◽  
Vol 64 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Yoshihiro Izumil ◽  
Kanji Ono
Keyword(s):  
Life Cycle ◽  
Dna Content ◽  
Plastid Dna ◽  
Anthoceros Punctatus

Nuclear Volumes and Dna Content of Three Stages in the Life Cycle of Armillaria Bulbosa

Mycologia ◽  
1986 ◽  
Vol 78 (6) ◽  
pp. 967-968 ◽  
Author(s):  
Diane Cope Peabody ◽  
Robert B. Peabody
Keyword(s):  
Life Cycle ◽  
Dna Content ◽  
Three Stages

scholarly journals Cloning of abundant mRNA species present during conjugation of Tetrahymena thermophila: identification of mRNA species present exclusively during meiosis.

1983 ◽  
Vol 3 (10) ◽  
pp. 1857-1865 ◽  
Author(s):  
D W Martindale ◽  
P J Bruns
Keyword(s):  
Life Cycle ◽  
Meiotic Prophase ◽  
Tetrahymena Thermophila ◽  
Cross Hybridization ◽  
Colony Hybridization ◽  
Mrna Species ◽  
Cdna Sequences ◽  
Rna Transcripts ◽  
Rna Probes ◽  
Labeled Rna

A cDNA library was constructed by using as a template the RNA present during the meiotic prophase of Tetrahymena thermophila. Clones containing cDNA sequences homologous to moderately abundant to abundant transcripts were detected by colony hybridization and confirmed by hybridizing purified cDNA plasmids on filters with labeled RNA probes. Eighteen clones were isolated, and the sizes of their cDNA inserts were determined. Cross-hybridization of individual cDNA plasmid pairs showed that each of these clones contained cDNA that was homologous to one of eight different RNA transcripts. The sizes of the eight RNA transcripts and the stages of the T. thermophila life cycle during which they were present were determined by hybridizing nick-translated cDNA probes against denatured, electrophoresed RNA from various stages. Clones were identified that contained sequences homologous to RNAs present only in early conjugation (meiosis); other clones contained sequences homologous to RNAs which were abundant during conjugation but present at other stages as well. One clone contained a sequence homologous to an RNA that was abundantly present only in nongrowing cells.

scholarly journals Climate effects on life cycle variation and population genetic architecture of the black bean aphid, Aphis fabae

2011 ◽  
Vol 20 (19) ◽  
pp. 4165-4181 ◽  
Author(s):  
CHRISTOPH SANDROCK ◽  
JABRAEIL RAZMJOU ◽  
CHRISTOPH VORBURGER
Keyword(s):  
Life Cycle ◽  
Population Genetic ◽  
Genetic Architecture ◽  
Aphis Fabae ◽  
Climate Effects ◽  
Black Bean ◽  
Cycle Variation ◽  
Black Bean Aphid

TATA-binding protein and nuclear differentiation in Tetrahymena thermophila

1994 ◽  
Vol 14 (1) ◽  
pp. 723-734
Author(s):  
L A Stargell ◽  
M A Gorovsky
Keyword(s):  
Life Cycle ◽  
Amino Acid ◽  
Rna Polymerase Ii ◽  
Tetrahymena Thermophila ◽  
Polyclonal Antibodies ◽  
Binding Protein ◽  
Tata Binding Protein ◽  
Gene Encoding ◽  
Nuclear Differentiation

Unambiguous TATA boxes have not been identified in upstream sequences of Tetrahymena thermophila genes analyzed to date. To begin a characterization of the promoter requirements for RNA polymerase II, the gene encoding TATA-binding protein (TBP) was cloned from this species. The derived amino acid sequence for the conserved C-terminal domain of Tetrahymena TBP is one of the most divergent described and includes a unique 20-amino-acid C-terminal extension. Polyclonal antibodies generated against a fragment of Tetrahymena TBP recognize a 36-kDa protein in macronuclear preparations and also cross-react with yeast and human TBPs. Immunocytochemistry was used to examine the nuclear localization of TBP during growth, starvation, and conjugation (the sexual phase of the life cycle). The transcriptionally active macronuclei stained at all stages of the life cycle. The transcriptionally inert micronuclei did not stain during growth or starvation but surprisingly stained with anti-TBP throughout early stages of conjugation. Anti-TBP staining disappeared from developing micronuclei late in conjugation, corresponding to the onset of transcription in developing macronuclei. Since micronuclei do not enlarge or divide at this time, loss of TBP appears to be an active process. Thus, the transcriptional differences between macro- and micronuclei that arise during conjugation are associated with the loss of a major component of the basal transcription apparatus from developing micronuclei rather than its appearance in developing macronuclei.

scholarly journals TATA-binding protein and nuclear differentiation in Tetrahymena thermophila.

1994 ◽  
Vol 14 (1) ◽  
pp. 723-734 ◽  
Author(s):  
L A Stargell ◽  
M A Gorovsky
Keyword(s):  
Life Cycle ◽  
Amino Acid ◽  
Rna Polymerase Ii ◽  
Tetrahymena Thermophila ◽  
Polyclonal Antibodies ◽  
Binding Protein ◽  
Tata Binding Protein ◽  
Gene Encoding ◽  
Nuclear Differentiation

Unambiguous TATA boxes have not been identified in upstream sequences of Tetrahymena thermophila genes analyzed to date. To begin a characterization of the promoter requirements for RNA polymerase II, the gene encoding TATA-binding protein (TBP) was cloned from this species. The derived amino acid sequence for the conserved C-terminal domain of Tetrahymena TBP is one of the most divergent described and includes a unique 20-amino-acid C-terminal extension. Polyclonal antibodies generated against a fragment of Tetrahymena TBP recognize a 36-kDa protein in macronuclear preparations and also cross-react with yeast and human TBPs. Immunocytochemistry was used to examine the nuclear localization of TBP during growth, starvation, and conjugation (the sexual phase of the life cycle). The transcriptionally active macronuclei stained at all stages of the life cycle. The transcriptionally inert micronuclei did not stain during growth or starvation but surprisingly stained with anti-TBP throughout early stages of conjugation. Anti-TBP staining disappeared from developing micronuclei late in conjugation, corresponding to the onset of transcription in developing macronuclei. Since micronuclei do not enlarge or divide at this time, loss of TBP appears to be an active process. Thus, the transcriptional differences between macro- and micronuclei that arise during conjugation are associated with the loss of a major component of the basal transcription apparatus from developing micronuclei rather than its appearance in developing macronuclei.

Sign in / Sign up

Export Citation Format

Share Document