Collagen gel culture system and analysis of estrogen effects on mammary carcinogenesis

1984 ◽  
Vol 55 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Satyabrata Nandi ◽  
Walter Imagawa ◽  
Yasuhiro Tomooka ◽  
Michael F. McGrath ◽  
Marc Edery
Keyword(s):  
Culture System ◽  
Collagen Gel ◽  
Mammary Carcinogenesis ◽  
Collagen Gel Culture ◽  
Estrogen Effects ◽  
Gel Culture

Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System

2012 ◽  
Vol 21 (9) ◽  
pp. 2021-2032 ◽  
Author(s):  
Silvia Claros ◽  
Noela Rodríguez-Losada ◽  
Encarnación Cruz ◽  
Enrique Guerado ◽  
José Becerra ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Progenitor Cell ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Cell Populations ◽  
Collagen Gel Culture ◽  
Gel Culture

scholarly journals 3P183 Responses of fibroblasts against fluid flow stimuli in a three-dimensional collagen gel culture system(Cell biology,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

2014 ◽  
Vol 54 (supplement1-2) ◽  
pp. S279
Author(s):  
Saito Natsumi ◽  
Adachi Hiroaki ◽  
Tanaka Hiroshi ◽  
Nakata Satoru ◽  
Kawada Norifumi ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Annual Meeting ◽  
Cell Biology ◽  
Culture System ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Collagen Gel Culture ◽  
Biophysical Society ◽  
System Cell ◽  
Gel Culture

scholarly journals Osteogenic cells form mineralized particles, a few μm in size, in a 3D collagen gel culture

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7889 ◽  
Author(s):  
Takanori Kihara ◽  
Chiya Umezu ◽  
Karin Sawada ◽  
Yukari Furutani
Keyword(s):  
Culture System ◽  
Culture Media ◽  
Collagen Gel ◽  
Matrix Vesicles ◽  
Osteogenic Cells ◽  
Collagen Gel Culture ◽  
3D Collagen ◽  
Gel Culture

Osteogenic cells form mineralized matrices in vitro, as well as in vivo. The formation and shape of the mineralized matrices are highly regulated by the cells. In vitro formation of mineralized matrices by osteogenic cells can be a model for in vivo osteogenesis. In this study, using a three-dimensional (3D) collagen gel culture system, we developed a new in vitro model for the formation of mineralized particles, a few µm in size, by the osteogenic cells. Human osteosarcoma (HOS) cells formed spherical mineralized matrices (about 12 µm) at approximately 7 days when cultured with β-glycerophosphate (β-GP)-containing culture media on 2D tissue culture plates. Alternately, when they were cultured in a 3D collagen gel containing β-GP, they formed mineralized particles with about 1.7 µm in the gel at approximately 3 days. Calcium precipitation in the gel was evaluated by measuring the gel turbidity. This type of mineralization of HOS cells, which formed mineralized particles inside the gel, was also observed in a peptide-based hydrogel culture. The mineralized particles were completely diminished by inhibiting the activity of Pit-1, phosphate cotransporter, of the HOS cells. When mouse osteoblast-like MC3T3-E1 cells, which form large and flat mineralized matrices in 2D osteogenic conditions at approximately 3 weeks of culture, were cultured in a 3D collagen gel, they also formed mineralized particles in the gel, similar to those in HOS cells, at approximately 18 days. Thus, osteogenic cells cultured in the 3D collagen gel form mineralized particles over a shorter period, and the mineralization could be easily determined by gel turbidity. This 3D gel culture system of osteogenic cells acts as a useful model for cells forming particle-type mineralized matrices, and we assume that the mineralized particles in the 3D hydrogel are calcospherulites, which are derived from matrix vesicles secreted by osteogenic cells.

scholarly journals Reconstruction Culture System Simulating Human Synovium: A Three-dimensional Collagen Gel Culture of Synoviocytes.

1997 ◽  
Vol 22 (4) ◽  
pp. 443-453
Author(s):  
Katsuki Taguchi ◽  
Torn Hirano ◽  
Katsuro Iwasaki ◽  
Hajime Sugihara
Keyword(s):  
Culture System ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Collagen Gel Culture ◽  
Gel Culture

Productivity of Thyroglobulin and Thyroid Hormone on Human Thyroid Cell in Collagen Gel Culture

1995 ◽  
Vol 22 (2) ◽  
pp. 120-127 ◽  
Author(s):  
Takeshi Kusunoki ◽  
Shozo Nishida ◽  
Masahiro Koezuka ◽  
Kiyotaka Murata ◽  
Takanori Tomura
Keyword(s):  
Thyroid Hormone ◽  
Collagen Gel ◽  
Human Thyroid ◽  
Thyroid Cell ◽  
Collagen Gel Culture ◽  
Human Thyroid Cell ◽  
Gel Culture
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