Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain of Pasteurella multocida

1996 ◽  
Vol 20 (3) ◽  
pp. 195-204 ◽  
Author(s):  
R. S. Conrad ◽  
C. Galanos ◽  
F. R. Champlin
Author(s):  
Mamta Choudhary ◽  
Binod Kumar Choudhary ◽  
Ratan Chandra Ghosh

Swine pasteurellosis is usually observed in descript as well as nondescript pigs imparting in huge economic losses to the pig producers. The disease is characterized by pyrexia, dullness, staggering gait, anorexia, serous nasal discharge and dyspnoea. Case fatality rate may as high as 95% in adult animals and 100% in piglets. Typical lesions of oedematous swellings may remarkably visible in the pharyngeal region, these swellings spread to the ventral cervical region and brisket of pigs. Gross lesions include severe pneumonia and haemorrhages in lungs, petechial haemorrhages on serous membranes and other visceral organs. Lymph nodes usually get enlarged, oedematous and haemorrhagic. The blood smears from heart blood and tissue impression smears reveal teaming numbers of bipolar organisms indicating the presence of Pasteurella spp., the etiological organism. The bacteriological isolation and characterization of causative agent should be ruled out to identify by Gram’ staining for purity and bipolar morphology and biochemical characterization of the organisms. Molecular characterization necessitates to confirm Pasteurella multocida along with capsular types of the organism. Histopathological examination of lungs usually reveals typical fibrinous bronchopneumonia, multifocal suppuration and pleural thickening. Heart of some pigs may show presence of thrombi, haemorrhages and necrosed myocardium.


Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.


2014 ◽  
Vol 3 (3) ◽  
pp. 218-225
Author(s):  
R. G. Somkuwar ◽  
M. A. Bhange ◽  
A. K. Upadhyay ◽  
S. D. Ramteke

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.


2020 ◽  
Vol 52 (6) ◽  
Author(s):  
M. Inam Ul Haq ◽  
Syed Afraz Ali ◽  
Gulshan Irshad ◽  
M Azam Khan ◽  
Shoaib Ismail

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