Activity and quantity of ribulose bisphosphate carboxylase-and phosphoenolpyruvate carboxylase-protein in two Crassulacean acid metabolism plants in relation to leaf age, nitrogen nutrition, and point in time during a day/night cycle

Planta ◽  
1982 ◽  
Vol 154 (4) ◽  
pp. 309-317 ◽  
Author(s):  
Klaus Winter ◽  
Joyce G. Foster ◽  
Mark R. Schmitt ◽  
Gerald E. Edwards
Keyword(s):  
Phosphoenolpyruvate Carboxylase ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Nitrogen Nutrition ◽  
Leaf Age ◽  
Ribulose Bisphosphate Carboxylase ◽  
Ribulose Bisphosphate ◽  
Bisphosphate Carboxylase

Étude comparative du métabolisme photosynthétique des feuilles et des gousses de soja Glycine max

1986 ◽  
Vol 64 (8) ◽  
pp. 1542-1548 ◽  
Author(s):  
J. C. Latché ◽  
G. Bailly-Fenech ◽  
J. Grima-Pettenati ◽  
G. Cavalié
Keyword(s):  
Carbon Fixation ◽  
Leaf Age ◽  
Net Photosynthesis ◽  
Glycolate Oxidase ◽  
Activity Measurement ◽  
Growth Stages ◽  
Ribulose Bisphosphate Carboxylase ◽  
Ribulose Bisphosphate ◽  
Carboxylase Activity ◽  
Bisphosphate Carboxylase

Changes in photosynthetic carbon fixation processes were comparatively studied in soybean leaves and pods harvested at different growth stages. Newly fully expanded leaves exhibited both the highest 14CO2 assimilation rate and the maximum ribulose bisphosphate carboxylase levels. Amino acids biosynthesis was more important in young tissues and this result agreed with the evolution of nitrate reductase activities. The radiocarbon distribution in glycine and serine suggested that photorespiratory metabolism increases with leaf age; the activity of glycolate oxidase was found to be significantly lower in younger leaves than in mature ones. Net photosynthesis, chlorophyll content, and ribulose bisphosphate carboxylase activity were low in isolated pods compared to leaves. However, the study of photosynthate translocations within the pod revealed that the pod wall could contribute to the carbon nutrition of the seeds. Soluble compounds labelled after 14CO2 incorporation and glycolate oxidase activity measurement indicated that organic acids biosynthesis and photorespiratory metabolism are relatively higher in pods than in leaves.

Capacity of Enzymes of the Euphorbiacea Aleurites montana Involved in CO2- Fixation, Compared to Plants Having C3-, C4- and Crassulacean Acid Metabolism

2000 ◽  
Vol 55 (5-6) ◽  
pp. 383-391 ◽  
Author(s):  
Norbert Grotjohann ◽  
Ping He ◽  
Georg H. Schmid
Keyword(s):  
Sugar Cane ◽  
Malic Enzyme ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Saccharum Officinarum ◽  
Light Period ◽  
Ribulose Bisphosphate Carboxylase ◽  
Bisphosphate Carboxylase ◽  
Efficient Uptake ◽  
Low Activity

Capacities of phosphoenolpyruvate carboxylase (PEP-Co), ribulose bisphosphate carboxylase (Rubisco), NADP+ malic enzyme (ME) and of malate dehydrogenase (MDH) were measured in the Euphorbiacea Aleurites montana, grown under 700 ppm CO2 for four weeks prior to enzyme extraction. For comparison Bryophyllum daigremontiana (CAM), Saccharum officinarum (C4) and Capsicum frutescens (C3) were treated in the same way. PEP-Co capacity of Aleurites was in the range of 12-, that of Capsicum approx. 26 nmol × min-1 × mg protein-1, without significant influence of the light period or CO2-treatment. In contrast, the activity of the enzyme from Saccharum was. depending on the duration of light, 160- respectively 96 times higher than that of the tung-oil tree. In Bryophyllum a rather low activity in the morning was increased during the day to approx. 230 nmol × min-1 × mg protein-1 in plants grown in the greenhouse and to approx. 115 nmol × min-1 × mg protein-1 in those from the growth chamber. Malate was hardly detectable in extracts of Aleurites, whereas it was high in Bryophyllum, depending on the light period. The ratio of average PEP-Co to Rub-Co capacity was high for the CAM-plant (20:1), somewhat lower for sugar cane (10:1), but almost at equality for Aleurites (0.9:1) and chilli (0.8:1). For the NADP+ malic enzyme, low capacity (20 to 28 nmol x min-1 × mg protein-1) was found for Aleurites and for Capsicum, whereas it was 10 to 17 times higher in Saccharum. In Bryophyllum, the activity was up to 80 nmol × min-1 × mg protein , dependent on light period. MDH capacity was extremely high in all plants investigated. Highest rates (10-20 μmol × min-1 × mg protein-1), were obtained for Bryophyllum, followed by sugar cane and Capsicum with 5 -8 μmol × min-1 x mg protein-1. Again, the lowest capacity was found in extracts of Aleurites with approx. 1.3 to 1.6 μmol × min-1 × m protein-1. Thus, in Aleurites montana no indication for C4- or Crassulacean acid metabolism was obtained. Therefore, the earlier observed very efficient uptake of CO2 cannot be explained by a high expression of the PEP-Co protein, known to occur in CAM- and C4-plants.

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