Inhibitory effect of two newer antidepressants, Lu 5-003 and Lu 3-010, on serotonin uptake in human blood platelets in vitro

SummaryThe present paper is part of a study on the evaluation of the active serotonin uptake and the response to hypotonic shock as in vitro viability tests for human blood platelets.The extraction and fluorometric assay of serotonin in plasma and in platelets were further automated by the introduction of a dialyzer in the earlier described system. Furthermore, the influences of some external factors on both tests were investigated.The serotonin uptake as well as the hypotonic shock response were pH dependent and especially the latter was rather sensitive to small pH changes. Changes in the Na+ : K+ ratio were also found to influence both criteria significantly.The serotonin uptake appeared to be linearly dependent on the platelet number while, within a range of 200 X 103 - 450 X 103 platelets per fxl, the slope of the hypotonic shock response was the same.

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Preliminary studies on the effect of dothiepin and its metabolites on serotonin uptake by human blood platelets in vitro

Clinical Pharmacology in Psychiatry ◽

10.1007/978-1-349-05929-4_12 ◽

1981 ◽

pp. 155-160

Author(s):

Trevor R. Norman ◽

Henry Cheng ◽

Graham D. Burrows

Keyword(s):

Human Blood ◽

Blood Platelets ◽

Serotonin Uptake ◽

Human Blood Platelets

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Staurosporine both activates and inhibits serine/threonine kinases in human platelets

Biochemical Journal ◽

10.1042/bj2750301 ◽

1991 ◽

Vol 275 (2) ◽

pp. 301-306 ◽

Cited By ~ 25

Author(s):

M Kocher ◽

K J Clemetson

Keyword(s):

Signal Transduction ◽

Protein Kinases ◽

Human Blood ◽

Blood Platelets ◽

Inhibitory Effect ◽

Human Platelets ◽

And Control ◽

Human Blood Platelets ◽

Selection Of

The effects of staurosporine on a selection of protein kinases were investigated with thrombin-stimulated and control human blood platelets. The results demonstrate that staurosporine (1 microM) can lead to activation of certain protein kinases in intact platelets and has a general inhibitory effect on the renaturable protein kinases in vitro. New candidates for protein kinases involved in signal transduction are identified.

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The role of nitric oxide in aspirin induced thrombolysis in vitro and the purification of aspirin activated nitric oxide synthase from human blood platelets

The FASEB Journal ◽

10.1096/fasebj.21.5.a625-a ◽

2007 ◽

Vol 21 (5) ◽

Author(s):

Nighat Kahn ◽

Asru K Sinha

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Human Blood ◽

Blood Platelets ◽

Oxide Synthase ◽

Human Blood Platelets

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Survival and recovery of human platelets stored for five days in a non- plasma medium

Blood ◽

10.1182/blood.v67.3.672.672 ◽

1986 ◽

Vol 67 (3) ◽

pp. 672-675 ◽

Cited By ~ 44

Author(s):

GA Adams ◽

SD Swenson ◽

G Rock

Keyword(s):

Platelet Aggregation ◽

Human Blood ◽

Blood Platelets ◽

Human Platelets ◽

Plasma Medium ◽

Release Reaction ◽

In Vivo Recovery ◽

Human Blood Platelets

Abstract Human blood platelets were stored for five days as concentrates in 60 mL of: (a) plasma; (b) non-plasma medium with anticoagulant; and (c) non-plasma medium without anticoagulant. All preparations were equally functional when tested for platelet aggregation and release reaction in response to single agonist or synergistic pairs of agonists in vitro. Platelets stored in non-plasma medium with anti-coagulant had lower kallikrein, fibrino(gen)peptide A, lactate, and beta-thromboglobulin than did plasma controls after five days. In vivo recovery and survival of platelets stored in non-plasma medium with anticoagulant were 51.2% +/- 4.3% and 8.7 +/- 0.3 days, respectively, which were not statistically different from plasma controls of 39.2% +/- 4.9% and 7.2 +/- 0.8 days, respectively. It is concluded that platelets can be stored for five days in a non-plasma medium and still have good in vivo recoveries and survivals.

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