Adsorption of Carbon-14 Dextran to Human Blood Platelets and Red Blood Cells, in Vitro

Summary1. The influence of 0.025 M, 0.1 M, 0.2 M sodium oxalate and of 0.2 M sodium citrate on the stability of human blood platelets was studied.2. A diminution of the resistance of platelets to glass in the 0.025 M and 0.1.M sodium oxalate blood samples was observed.3. This effect of oxalate was not observed when red blood cells were not present.

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Use of pectic polysaccharides for cryopreservation of biological objects

Archives of Biological Sciences ◽

10.2298/abs1403025p ◽

2014 ◽

Vol 66 (3) ◽

pp. 1025-1033 ◽

Cited By ~ 5

Author(s):

Т.V. Polezhaeva ◽

О.О. Zaitseva ◽

А.N. Khudyakov ◽

D.S. Laptev ◽

V.V. Golovchenko ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Red Blood Cells ◽

Human Blood ◽

Blood Cells ◽

Blood Platelets ◽

Pectic Polysaccharides ◽

Pectic Polysaccharide ◽

Biological Objects ◽

Human Blood Platelets

The protectant activity of pectic polysaccharides derived from various plants was studied on Saccharomyces cerevisiae yeast-like fungi, human blood platelets and leukocytes, and the antihemolytic action of the same compounds was studied on red blood cells. The feasibility of cryopreservation of biological objects in the environment of pectic polysaccharide- containing cryoprotectant solutions was demonstrated.

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Interaction of Influenza Virus with Blood Platelets

Blood ◽

10.1182/blood.v28.2.213.213 ◽

1966 ◽

Vol 28 (2) ◽

pp. 213-228 ◽

Cited By ~ 66

Author(s):

HIDEO TERADA ◽

MARIO BALDINI ◽

SHIRLEY EBBE ◽

MORTON A. MADOFF

Keyword(s):

Influenza Virus ◽

Human Blood ◽

Blood Cells ◽

Blood Platelets ◽

Live Virus ◽

Human Red Blood Cells ◽

Virus Adsorption ◽

Human Blood Platelets

Abstract The interaction of human blood platelets with influenza virus (PR-8) was studied in vitro and in vivo. It was found that "live" influenza virus was rapidly adsorbed onto human blood platelets at 4 C. and completely eluted at 37 C. "Dead" virus was adsorbed at 4 C. but not eluted at 37 C. unless the platelets were treated with RDE (receptor destroying enzyme). Adorption of virus also occurred at tem peratures above 4 C. (from 20 to 37 C.). However, while adsorption was maintained throughout incubation at 4 C., slow elution occurred after 30 to 90 minutes incubation at 26 to 37 C. Storage of the platelets for lengthy intervals at 4 C. or coating of the platelets with macromolecules did not interfere with virus adsorption. After one cycle of adsorption-elution, blood platelets could not adsorb virus again. Treatment with RDE greatly reduced virus adsorption. During the process of virus adsorption, prominent platelet clumping occurred. During elution, clumping remained unchanged, and gross alterations in morphology of the platelets were observed. In the process of virus adsorption-elution, large numbers of platelets were lysed. Comparative experiments were performed simultaneously with human red blood cells (RBC) and identical results were obtained as with blood platelets. However, the extent of adsorption of live virus was equal for platelets and RBC only when the relationship between platelet number and RBC number in the preparations used was 6:1. This suggested a direct proportion between the surface area of both platelet and RBC and the number of available virus receptors. Virus suspensions infused into rabbits produced a sharp and sustained drop of the platelet count. Survival of radioactively labeled platelets treated with virus prior to infusion was markedly shortened with live virus and was only slightly reduced with dead virus. It is suggested from these experiments that blood platelets, as other blood cells, may serve as carriers of viruses in the circulation and that in this process the platelets are damaged and partially destroyed.

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The role of nitric oxide in aspirin induced thrombolysis in vitro and the purification of aspirin activated nitric oxide synthase from human blood platelets

The FASEB Journal ◽

10.1096/fasebj.21.5.a625-a ◽

2007 ◽

Vol 21 (5) ◽

Author(s):

Nighat Kahn ◽

Asru K Sinha

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Human Blood ◽

Blood Platelets ◽

Oxide Synthase ◽

Human Blood Platelets

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Survival and recovery of human platelets stored for five days in a non- plasma medium

Blood ◽

10.1182/blood.v67.3.672.672 ◽

1986 ◽

Vol 67 (3) ◽

pp. 672-675 ◽

Cited By ~ 44

Author(s):

GA Adams ◽

SD Swenson ◽

G Rock

Keyword(s):

Platelet Aggregation ◽

Human Blood ◽

Blood Platelets ◽

Human Platelets ◽

Plasma Medium ◽

Release Reaction ◽

In Vivo Recovery ◽

Human Blood Platelets

Abstract Human blood platelets were stored for five days as concentrates in 60 mL of: (a) plasma; (b) non-plasma medium with anticoagulant; and (c) non-plasma medium without anticoagulant. All preparations were equally functional when tested for platelet aggregation and release reaction in response to single agonist or synergistic pairs of agonists in vitro. Platelets stored in non-plasma medium with anti-coagulant had lower kallikrein, fibrino(gen)peptide A, lactate, and beta-thromboglobulin than did plasma controls after five days. In vivo recovery and survival of platelets stored in non-plasma medium with anticoagulant were 51.2% +/- 4.3% and 8.7 +/- 0.3 days, respectively, which were not statistically different from plasma controls of 39.2% +/- 4.9% and 7.2 +/- 0.8 days, respectively. It is concluded that platelets can be stored for five days in a non-plasma medium and still have good in vivo recoveries and survivals.

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Inhibitory effect of two newer antidepressants, Lu 5-003 and Lu 3-010, on serotonin uptake in human blood platelets in vitro

Psychopharmacology ◽

10.1007/bf00402095 ◽

1970 ◽

Vol 17 (1) ◽

pp. 94-99 ◽

Cited By ~ 11

Author(s):

O. Lingjærde

Keyword(s):

Human Blood ◽

Blood Platelets ◽

Inhibitory Effect ◽

Serotonin Uptake ◽

Human Blood Platelets

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THE PRODUCTION OF PURPURA BY DERIVATIVES OF PNEUMOCOCCUS

Journal of Experimental Medicine ◽

10.1084/jem.43.1.111 ◽

1926 ◽

Vol 43 (1) ◽

pp. 111-106

Author(s):

Hobart A. Reimann ◽

Louis A. Julianelle

Keyword(s):

Red Blood Cells ◽

Hemolytic Activity ◽

Blood Cells ◽

Blood Platelets ◽

White Blood Cells ◽

Red Cells ◽

Severe Anemia ◽

Marked Diminution ◽

Derivatives Of

A study has been made of the variation in number of the blood platelets, and the red and white blood cells of white mice injected with pneumococcus extract. The blood platelets were greatly diminished after the injection, the greatest decrease usually occurring after 24 hours. Purpuric lesions usually developed when the number of blood platelets became less than 500,000 per c.mm. Regeneration of the platelets was accomplished by the 4th to the 9th day but there was an overregeneration and the return to normal did not take place until 2 weeks had elapsed. The red cells were also greatly reduced in number, but the rate of their destruction and regeneration was somewhat slower than that of the platelets. The leucocytes were slightly if at all influenced by the pneumococcus extract. Pneumococcus extracts were shown to be thrombolytic and hemolytic. Heat destroyed the activity of both the lysins in vitro. Heated extract produced purpura in mice but did not cause a severe anemia. Extracts adsorbed with either blood platelets or red blood cells showed a marked diminution in their thrombolytic and hemolytic activity in vitro. Such extracts, however, produced purpura as well as severe anemia and thrombopenia in mice.

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