Ultrastructural studies of the bovine Graafian Follicle and corpus luteum

1968 ◽  
Vol 91 (4) ◽  
pp. 554-573 ◽  
Author(s):  
Janis Priedkalns ◽  
Alvin F. Weber
1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.


The mares chosen for the investigation of the changes in the reproductive organs during the oestrous cycle were kept under observation for some time before they were killed. The duration of the heat period in these animals was 7 days and the length of the dioestrus was 16 days. Ovulation takes place at about a day before the end of oestrus. The size of the ovary during the oestrous cycle is chiefly influenced by the growing Graafian follicle. The number of follicles present at different stages varies greatly. The numerous small follicles present at the beginning of oestrus disappear later in the cycle; it is suggested that this may be due to the lack of follicle-stimulating hormone. The colour of the corpus luteum varies greatly at different stages of the cycle. The rupture of the follicle is associated with some bleeding. The active stage of the corpus luteum is very short, and the maximum diameter of the corpus luteum seems to be always below that of the Graafian follicle. The greater development of the Graafian follicle, with its secretion of oestrin, in the mare leads to its playing a more important role than in the cow and the sow, in which species the corpus luteum takes a m ore dominant part in the cycle. It appears that the much longer oestrus in the mare than in the cow is due to the longer time required by the follicle to come to the surface and to break through. This is probably due to the peculiar structure of the ovary in the mare, since the ovulation, which is spontaneous, can only occur in the small ovulation fossa. No pronounced secretion stage occurs during oestrus in the Fallopian tubes.


1976 ◽  
Vol 83 (2) ◽  
pp. 402-409 ◽  
Author(s):  
D. T. Baird ◽  
R. J. Scaramuzzi

ABSTRACT The secretion of oestradiol and androstenedione was investigated in sheep by cannulating both ovarian veins in situ. The mean ovarian blood flow (ml/min ± se) was significantly higher on the side containing the corpus luteum (7.67 ± 1.36) than on the contralateral side (4.93 ± 0.85). The mean secretion (ng/min ± se) of oestradiol (1.09 ± 0.36) and androstenedione (1.52 ± 0.16) was higher on the side containing at least one large follicle (diameter > 4 mm) than on the contralateral side (0.19 ± 0.05 and 0.62 ± 0.21, respectively). The secretion of oestradiol was unchanged following enucleation of the corpus luteum but fell to very low levels (0.062 ± 0.022 ng/min) after enucleation of follicles diameter > 4 mm. The secretion of androstenedione fell significantly after enucleation of both corpus luteum and large follicles. These results suggest that during the luteal phase in the sheep oestradiol is secreted virtually exclusively by the largest non-atretic Graafian follicle. Androstenedione in contrast arises from both follicle, corpus luteum and possibly stroma.


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