scholarly journals Plasma islet amyloid polypeptide (Amylin) levels and their responses to oral glucose in Type 2 (non-insulin-dependent) diabetic patients

Diabetologia ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 129-132 ◽  
Author(s):  
T. Sanke ◽  
T. Hanabusa ◽  
Y. Nakano ◽  
C. Oki ◽  
K. Okai ◽  
...  
Keyword(s):  
Islet Amyloid Polypeptide ◽  
Oral Glucose ◽  
Diabetic Patients ◽  
Islet Amyloid ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

scholarly journals An Improved Method for the Determination of Islet Amyloid Polypeptide Levels in Plasma

1994 ◽  
Vol 31 (2) ◽  
pp. 165-170 ◽  
Author(s):  
K L van Hulst ◽  
W H L Hackeng ◽  
J W M Höppener ◽  
B C van Jaarsveld ◽  
M G Nieuwenhuis ◽  
...  
Keyword(s):  
Islet Amyloid Polypeptide ◽  
Cross Reactivity ◽  
Diabetic Patients ◽  
Improved Method ◽  
Standard Samples ◽  
Islet Amyloid ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

We describe an improved method for the determination of islet amyloid polypeptide (IAPP) levels in plasma. Plasma is first extracted with acid-acetone, followed by a specific and sensitive radioimmunoassay (RIA) for IAPP using rabbit-anti-human-IAPP serum. Recovery of synthetic IAPP from plasma was 82±6% ( n = 16). Standard samples, prepared in ‘hormone-free’ serum, were also extracted with acid-acetone. Displacement curves of serially diluted acid-acetone extracted plasma samples were parallel to the standard curve. The lower detection limit of the RIA was 2·3 ± 0·1 fmol/sample ( n = 5). Intra-assay variations for IAPP concentrations of 4, 17 and 32 pM were 16·3% ( n = 10), 9·2% ( n = 10) and 6·2% ( n = 10); inter-assay variations were 35·9% ( n = 14), 19·9% ( n = 15) and 15·4% ( n = 15), respectively. Non-stimulated IAPP levels ranged from 2·4 to 12 pM (mean 6±4pM, n = 10) in healthy control subjects. IAPP was not detectable in type 1 (insulin-dependent) diabetic patients before and after glucagon administration. In type 2 (non-insulin-dependent) diabetic patients basal levels ranged from 2·2 to 14·5 pM and glucagon-stimulated levels ranged from 2·2 to 38·9 pM. The increase in IAPP varied from 0 to 24·4 pM. The anti-human-IAPP serum had full cross-reactivity with rat IAPP (= mouse IAPP). Transgenic mice overexpressing the human IAPP gene showed elevated plasma IAPP levels as compared to (non-transgenic) control mice. It is concluded that the method presented for the determination of IAPP in plasma is reliable and easy to perform, yielding reproducible results. The ability to measure plasma IAPP levels will help to elucidate the physiological function of IAPP as well as its pathophysiological role in type 2 diabetes mellitus.

scholarly journals A controlled trial of a high fibre, low fat and low sodium diet for mild hypertension in type 2 (non-insulin-dependent) diabetic patients

Diabetologia ◽  
1984 ◽  
Vol 27 (5) ◽  
pp. 522-526 ◽  
Author(s):  
P. M. Dodson ◽  
P. J. Pacy ◽  
P. Bal ◽  
A. J. Kubicki ◽  
R. F. Fletcher ◽  
...  
Keyword(s):  
Mild Hypertension ◽  
Controlled Trial ◽  
Diabetic Patients ◽  
Low Fat ◽  
Low Sodium Diet ◽  
Low Sodium ◽  
High Fibre ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

scholarly journals Cellular and humoural autoimmunity markers in Type 2 (non-insulin-dependent) diabetic patients with secondary drug failure

Diabetologia ◽  
1992 ◽  
Vol 35 (12) ◽  
pp. 1159-1164 ◽  
Author(s):  
A. V. Zavala ◽  
L. E. Fabiano de Bruno ◽  
A. I. Cardoso ◽  
A. H. Mota ◽  
M. Capucchio ◽  
...  
Keyword(s):  
Diabetic Patients ◽  
Insulin Dependent ◽  
Secondary Drug Failure ◽  
Insulin Dependent Diabetic
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